公开(公告)号：EP2271769A2

公开(公告)日：2011-01-12

申请号：EP09726214.1

申请日：2009-03-18

申请人： Indevr, Inc.

发明人： ROWLEN, Kathy, L. ,  TAYLOR, Amber, W.

IPC分类号： C12Q1/68 ,  B82B3/00

CPC分类号： C12Q1/6816 ,  C12Q1/6825 ,  C12Q2565/518 ,  C12Q2563/155 ,  C12Q2527/125

摘要： There is described a new class or type of initiators for polymerization as a means of signal enhancement, nanoparticle initiators, and methods for amplifying signal resulting from recognition events, thereby enhancing the detection of those recognition events. Methods include amplification achieved through polymerization using a nanoparticle initiator conjugated recognition element that is not consumed during the reaction. The polymer formed as a result of the absorption of light by the nanoparticle initiator and introduction of reactive species into a surrounding polymerizable monomer solution occurs in a spatially-limited region directly surrounding the nanoparticle initiator and is indicative of the recognition event(s). In one embodiment, a semiconductor quantum dot nanoparticle initiator is utilized. In another embodiment, a metal nanoparticle is utilized. In another embodiment, the signal is detected without instrumentation. In yet another embodiment, the signal is detected via a transmission-based instrument which captures an image of the formed polymer.