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    METHOD FOR PRODUCING NATURAL RUBBER BY USING RECOMBINANT MICROORGANISM
    2.
    发明公开
    METHOD FOR PRODUCING NATURAL RUBBER BY USING RECOMBINANT MICROORGANISM 审中-公开

    公开(公告)号：EP3323894A1

    公开(公告)日：2018-05-23

    申请号：EP16811916.2

    申请日：2016-06-15

    申请人： Ajou University Industry-Academic Cooperation Foundation

    发明人： LEE, Pyung Cheon ,  KIM, Jin Ho

    IPC分类号： C12N15/63 ,  C12N15/70 ,  C12N15/74 ,  C12N15/81 ,  C12P5/00

    摘要： A method for producing natural rubber by using a recombinant microorganism is disclosed. Particularly, disclosed is a method for producing natural rubber by using a recombinant microorganism, comprising the steps of: (a) manufacturing an expression vector capable of expressing a gene encoding a cis- prenyltransferase, which is a guayule-derived natural rubber synthetase represented by the amino acid sequence of SEQ ID NO: 2 or a Hevea brasiliensis- derived natural rubber synthetase represented by the amino acid sequence of SEQ ID NO: 6, and an expression vector capable of expressing a gene coding for a natural rubber precursor synthetase; (b) transforming a host microorganism with the expression vector; (c) culturing the transformed host microorganism; and (d) separating natural rubber from the cultured transformed host microorganism. The natural rubber obtained according to the method of the present invention has a white powder form identical to that of natural rubber, and shows an FT-IR spectrum pattern and the like, which are extremely similar to those of natural rubber.