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公开(公告)号：EP3868881A3
公开(公告)日：2021-11-03
申请号：EP20213383.1
申请日：2011-05-05
发明人： ALEKSEYEV, Viktor Yuryevich , AMIN, Neelam, S , AUGUSTYN, Katherine , CASCAO-PEREIRA, Luis Gustavo , COLLIER, Katherine, D. , ESTELL, David, A. , KELLIS JR., James, T , POULOSE, Ayrookaran, J. , SOUTER, Philip Frank , WARD, Glenn Steven , YAO, Jian
摘要： The present invention provides isolated subtilisin variants. Specifically, a subtilisin variant is a mature form having proteolytic activity and comprises an amino acid sequence comprising the combination of amino acid substitutions: T022R, S101G, S103A, V104I, A232V, and Q245R, wherein the variant is a variant of Bacillus lentus subtilisin GG36 protease comprising the amino acid sequence shown in SEQ ID NO:2, and wherein the variant has at least 90% amino acid sequence identity to the amino acid sequence of SEQ ID NO: 2, and wherein the amino acid positions of the subtilisin variant are numbered according to the numbering of corresponding amino acid positions in the amino acid sequence of B. amyloliquefaciens subtilisin BPN' set forth as SEQ ID NO: 1. In addition, the present invention provides compositions comprising these subtilisin variants. In some embodiments, the present invention provides cleaning compositions comprising at least one of these subtilisin variants and methods of cleaning using the cleaning compositions.
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公开(公告)号：EP2566960B1
公开(公告)日：2017-03-08
申请号：EP11720664.9
申请日：2011-05-05
发明人： SOUTER, Phillip, Frank , WARD, Glenn, Steven , POULOSE, Ayrookaran, Joseph , ESTELL, David, A. , KELLIS, James, T., Jr. , COLLIER, Katherine, D. , CASCÃO-PEREIRA, Luis, Gustavo , ALEKSEYEV, Viktor, Yuryevich , AMIN, Neelam, S. , YAO, Jian , AUGUSTYN, Katherine
CPC分类号： C12N9/54 , C11D3/386 , C11D3/38681
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3.发明公开COMPOSITIONS AND METHODS COMPRISING SUBTILISIN VARIANTS 有权ZUSAMMENSETZUNGEN UND VERFAHREN MIT SUBTILISINVARIANTEN
公开(公告)号：EP2566961A1
公开(公告)日：2013-03-13
申请号：EP11730124.2
申请日：2011-05-05
发明人： ALEKSEYEV, Viktor Yuryevich , AMIN, Neelam, S. , AUGUSTYN, Katherine , CASCAO-PEREIRA, Luis Gustavo , COLLIER, Katherine, D. , ESTELL, David, A. , KELLIS, JR., James, T. , POULOSE, Ayrookaran, J. , SOUTER, Philip Frank , WARD, Glenn Steven , YAO, Jian
CPC分类号： C12N9/54 , C11D3/386 , C11D3/38681
摘要： A method of treating and/or cleaning a surface, preferably a fabric surface comprising the steps of (i) contacting said surface with a composition in an aqueous wash liquor, said composition comprising an adjunct material and a protease variant wherein said protease variant is a variant of a parent protease said parent protease's sequence being at least 90% identical to the amino acid sequence of SEQ ID NO:1, wherein the amino acid positions of the protease variant are numbered according to the numbering of corresponding amino acid positions in the amino acid sequence of Bacillus amyloliquefaciens subtilisin BPN' shown in SEQ ID NO:2, wherein said protease variant comprises a mutation selected from T022A, T022R, T022Y, T022V, T022Q, T022L, T022W, and (ii) rinsing and/or drying the surface, wherein the temperature of the aqueous liquor is from 5 to 30°C.
摘要翻译： 一种处理和/或清洁表面，优选织物表面的方法，包括以下步骤：（i）使所述表面与组合物在水性洗涤液中接触，所述组合物包含辅料和蛋白酶变体，其中所述蛋白酶变体为 亲本蛋白酶的变体，所述亲本蛋白酶的序列与SEQ ID NO：1的氨基酸序列至少90％相同，其中蛋白酶变体的氨基酸位置根据氨基酸中相应氨基酸位置的编号进行编号 SEQ ID NO：2所示的解淀粉芽孢杆菌枯草杆菌蛋白酶BPN'的酸序列，其中所述蛋白酶变体包含选自T022A，T022R，T022Y，T022V，T022Q，T022L，T022W的突变，和（ii）漂洗和/或干燥表面 其中，所述含水液体的温度为5〜30℃。
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公开(公告)号：EP3868881A2
公开(公告)日：2021-08-25
申请号：EP20213383.1
申请日：2011-05-05
发明人： ALEKSEYEV, Viktor Yuryevich , AMIN, Neelam, S , AUGUSTYN, Katherine , CASCAO-PEREIRA, Luis Gustavo , COLLIER, Katherine, D. , ESTELL, David, A. , KELLIS JR., James, T , POULOSE, Ayrookaran, J. , SOUTER, Philip Frank , WARD, Glenn Steven , YAO, Jian
摘要： The present invention provides isolated subtilisin variants. Specifically, a subtilisin variant is a mature form having proteolytic activity and comprises an amino acid sequence comprising the combination of amino acid substitutions: T022R, S101G, S103A, V104I, A232V, and Q245R, wherein the variant is a variant of Bacillus lentus subtilisin GG36 protease comprising the amino acid sequence shown in SEQ ID NO:2, and wherein the variant has at least 90% amino acid sequence identity to the amino acid sequence of SEQ ID NO: 2, and wherein the amino acid positions of the subtilisin variant are numbered according to the numbering of corresponding amino acid positions in the amino acid sequence of B. amyloliquefaciens subtilisin BPN' set forth as SEQ ID NO: 1. In addition, the present invention provides compositions comprising these subtilisin variants. In some embodiments, the present invention provides cleaning compositions comprising at least one of these subtilisin variants and methods of cleaning using the cleaning compositions.
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公开(公告)号：EP2566961B1
公开(公告)日：2018-08-22
申请号：EP11730124.2
申请日：2011-05-05
发明人： ALEKSEYEV, Viktor Yuryevich , AMIN, Neelam, S. , AUGUSTYN, Katherine , CASCAO-PEREIRA, Luis Gustavo , COLLIER, Katherine, D. , ESTELL, David, A. , KELLIS, JR., James, T. , POULOSE, Ayrookaran, J. , SOUTER, Philip Frank , WARD, Glenn Steven , YAO, Jian
CPC分类号： C12N9/54 , C11D3/386 , C11D3/38681
摘要： A method of treating and/or cleaning a surface, preferably a fabric surface comprising the steps of (i) contacting said surface with a composition in an aqueous wash liquor, said composition comprising an adjunct material and a protease variant wherein said protease variant is a variant of a parent protease said parent protease's sequence being at least 90% identical to the amino acid sequence of SEQ ID NO:1, wherein the amino acid positions of the protease variant are numbered according to the numbering of corresponding amino acid positions in the amino acid sequence of Bacillus amyloliquefaciens subtilisin BPN' shown in SEQ ID NO:2, wherein said protease variant comprises a mutation selected from T022A, T022R, T022Y, T022V, T022Q, T022L, T022W, and (ii) rinsing and/or drying the surface, wherein the temperature of the aqueous liquor is from 5 to 30°C.
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公开(公告)号：EP2566960A1
公开(公告)日：2013-03-13
申请号：EP11720664.9
申请日：2011-05-05
发明人： SOUTER, Phillip, Frank , WARD, Glenn, Steven , POULOSE, Ayrookaran, Joseph , ESTELL, David, A. , KELLIS, James, T., Jr. , COLLIER, Katherine, D. , CASCÃO-PEREIRA, Luis, Gustavo , ALEKSEYEV, Viktor, Yuryevich , AMIN, Neelam, S. , YAO, Jian , AUGUSTYN, Katherine
CPC分类号： C12N9/54 , C11D3/386 , C11D3/38681
摘要： This invention relates to consumer products comprising proteases, particularly cold water proteases and processes for making and using such products. Such consumer products provide improved cleaning, whiteness and/or freshness. Such proteases are derived from a parent enzyme, for example Bacillus lentus, by substitution, insertion and/or deletion of one or more of the parent enzymes' amino acids.
摘要翻译： 一种处理和/或清洁表面，优选织物表面的方法，包括以下步骤：（i）使所述表面与组合物在水性洗涤液中接触，所述组合物包含辅料和蛋白酶变体，其中所述蛋白酶变体为 亲本蛋白酶的变体，所述亲本蛋白酶的序列与SEQ ID NO：1的氨基酸序列至少90％相同，其中蛋白酶变体的氨基酸位置根据氨基酸中相应氨基酸位置的编号进行编号 SEQ ID NO：2所示的解淀粉芽孢杆菌枯草杆菌蛋白酶BPN'的酸序列，其中所述蛋白酶变体包含选自T022A，T022R，T022Y，T022V，T022Q，T022L，T022W的突变，和（ii）漂洗和/或干燥表面 其中，所述含水液体的温度为5〜30℃。
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公开(公告)号：EP1025239B1
公开(公告)日：2008-01-23
申请号：EP98953936.6
申请日：1998-10-23
发明人： SCHELLENBERGER, Volker , KELLIS, James, T., Jr. , PAECH, Christian , NADHERNY, Joanne , NAKI, Donald, P. , POULOSE, Ayrookaran, J. , COLLIER, Katherine, D. , CALDWELL, Robert, M. , BAECK, André, C.
CPC分类号： C12N9/54 , A23G4/123 , A23K20/189 , A61K8/66 , A61K2800/86 , A61Q11/00 , A61Q19/00 , C11D3/386 , C11D3/38609 , C11D3/38618 , C11D3/3907 , C11D3/3945 , C12Y304/00 , C12Y304/21062
摘要： Novel protease variants derived from the DNA sequences of naturally-occurring or recombinant non-human proteases are disclosed. The variant proteases, in general, are obtained by in vitro modification of a precursor DNA sequence encoding the naturally-occurring or recombinant protease to generate the substitution of a plurality of amino acid residues in the amino acid sequence of a precursor protease. Such variant proteases have properties which are different from those of the precursor protease, such as altered wash performance. The substituted amino acid residue correspond to positions 62, 212, 230, 232, 252 and 257 of Bacillus amyloliquefaciens subtilisin.
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公开(公告)号：EP1027418A1
公开(公告)日：2000-08-16
申请号：EP98955109.8
申请日：1998-10-23
发明人： GHOSH, Chanchal, Kumar , BAECK, Andre, Cesar , OHTANI, Ryohei , BUSCH, Alfred , SHOWELL, Michael, Stanford , SCHELLENBERGER, Volker , KELLIS, James, T., Jr. , PAECH, Christian , NADHERNY, Joanne , NAKI, Donald, P. , COLLIER, Katherine, D. , CALDWELL, Robert, M. , POULOSE, Ayrookaran, J.
CPC分类号： C12N9/54 , A23G4/123 , A23K20/189 , A61K8/66 , A61K2800/86 , A61Q11/00 , A61Q19/00 , C11D3/386 , C11D3/38609 , C11D3/38618 , C11D3/3907 , C11D3/3945 , C12Y304/00 , C12Y304/21062
摘要： The present invention relates to cleaning compositions comprising a protease variant. One cleaning composition comprises a protease variant including a substitution of an amino acid residue with another naturally occurring amino acid residue at an amino acid residue position corresponding to position 103 of Bacillus amyloliquefaciens subtilisin in combination with a substitution of an amino acid residue with another naturally occurring amino acid residue at one or more amino acid residue positions corresponding to positions 1, 3, 4, 8, 9, 10, 12, 13, 16, 17, 18, 19, 20, 21, 22, 24, 27, 33, 37, 38, 42, 43, 48, 55, 57, 58, 61, 62, 68, 72, 75, 76, 77, 78, 79, 86, 87, 89, 97, 98, 99, 101, 102, 104, 106, 107, 109, 111, 114, 116, 117, 119, 121, 123, 126, 128, 130, 131, 133, 134, 137, 140, 141, 142, 146, 147, 158, 159, 160, 166, 167, 170, 173, 174, 177, 181, 182, 183, 184, 185, 188, 192, 194, 198, 203, 204, 205, 206, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 222, 224, 227, 228, 230, 232, 236, 237, 238, 240, 242, 243, 244, 245, 246, 247, 248, 249, 251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 265, 268, 269, 270, 271, 272, 274 and 275 of Bacillus amyloliquefaciens subtilisin; wherein when said protease variant includes a substitution of amino acid residues positions corresponding to positions 103 and 76, there is also a substitution of an amino acid residue at one or more amino acid residue positions other than amino acid residue positions corresponding to positions 27, 99, 101, 104, 107, 109, 123, 128, 166, 204, 206, 210, 216, 217, 218, 222, 260, 265 or 274 of Bacillus amyloliquefaciens subtilisin; and one or more cleaning adjunct materials. Another cleaning composition comprises a protease variant including a substitution of an amino acid residue with another naturally occurring amino acid residue at one or more amino acid residue positions corresponding to positions 62, 212, 230, 232, 252 and 257 of $(Bacillus amyloliquefaciens) subtilisin; and one or more cleaning adjunct materials. Methods for using the cleaning compositions are also provided.
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公开(公告)号：EP1025239A2
公开(公告)日：2000-08-09
申请号：EP98953936.6
申请日：1998-10-23
发明人： SCHELLENBERGER, Volker , KELLIS, James, T., Jr. , PAECH, Christian , NADHERNY, Joanne , NAKI, Donald, P. , POULOSE, Ayrookaran, J. , COLLIER, Katherine, D. , CALDWELL, Robert, M. , BAECK, André, C.
CPC分类号： C12N9/54 , A23G4/123 , A23K20/189 , A61K8/66 , A61K2800/86 , A61Q11/00 , A61Q19/00 , C11D3/386 , C11D3/38609 , C11D3/38618 , C11D3/3907 , C11D3/3945 , C12Y304/00 , C12Y304/21062
摘要： Novel protease variants derived from the DNA sequences of naturally-occurring or recombinant non-human proteases are disclosed. The variant proteases, in general, are obtained by in vitro modification of a precursor DNA sequence encoding the naturally-occurring or recombinant protease to generate the substitution of a plurality of amino acid residues in the amino acid sequence of a precursor protease. Such variant proteases have properties which are different from those of the precursor protease, such as altered wash performance. The substituted amino acid residue correspond to positions 62, 212, 230, 232, 252 and 257 of Bacillus amyloliquefaciens subtilisin.
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10.发明公开
公开(公告)号：EP1025194A1
公开(公告)日：2000-08-09
申请号：EP98955079.3
申请日：1998-10-23
发明人： GHOSH, Chanchal, Kumar , BAECK, Andre, Cesar , OHTANI, Ryohei , BUSCH, Alfred , SHOWELL, Michael, Stanford , SCHELLENBERGER, Volker , KELLIS, James, T., Jr. , PAECH, Christian , NADHERNY, Joanne , NAKI, Donald, P. , COLLIER, Katherine, D. , CALDWELL, Robert, M. , POULOSE, Ayrookaran, J.
CPC分类号： C12N9/54 , A23G4/123 , A23K20/189 , A61K8/66 , A61K2800/86 , A61Q11/00 , A61Q19/00 , C11D3/386 , C11D3/38609 , C11D3/38618 , C11D3/3907 , C11D3/3945 , C12Y304/00 , C12Y304/21062
摘要： The present invention relates to bleaching compositions comprising a protease variant. One bleaching composition comprises a protease variant including a substitution of an amino acid residue with another naturally occurring amino acid residue at an amino acid residue position corresponding to position 103 of Bacillus amyloliquefaciens subtilisin in combination with a substitution of an amino acid residue with another naturally occurring amino acid residue at one or more amino acid residue positions corresponding to positions 1, 3, 4, 8, 9, 10, 12, 13, 16, 17, 18, 19, 20, 21, 22, 24, 27, 33, 37, 38, 42, 43, 48, 55, 57, 58, 61, 62, 68, 72, 75, 76, 77, 78, 79, 86, 87, 89, 97, 98, 99, 101, 102, 104, 106, 107, 109, 111, 114, 116, 117, 119, 121, 123, 126, 128, 130, 131, 133, 134, 137, 140, 141, 142, 146, 147, 158, 159, 160, 166, 167, 170, 173, 174, 177, 181, 182, 183, 184, 185, 188, 192, 194, 198, 203, 204, 205, 206, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 222, 224, 227, 228, 230, 232, 236, 237, 238, 240, 242, 243, 244, 245, 246, 247, 248, 249, 251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 265, 268, 269, 270, 271, 272, 274, and 275 of Bacillus amyloliquefaciens subtilisin; wherein when said protease variant includes a substitution of amino acid residues at positions corresponding to positions 103 and 76, there is also a substitution of an amino acid residue at one or more amino acid residue positions other than amino acid residue positions corresponding to positions 27, 99, 101, 104, 107, 109, 123, 128, 166, 204, 206, 210, 216, 217, 218, 222, 260, 265, or 274 of Bacillus amyloliquefaciens subtilisin, a bleaching agent; and one or more cleaning adjunct materials. Another bleaching composition comprises a protease variant including a substitution of an amino acid residue with another naturally occurring amino acid residue at one or more amino acid residue positions corresponding to positions 62, 212, 230, 232, 252 and 257 of Bacillus amyloliquefaciens subtilisin; a bleaching agent; and one or more cleaning adjunct materials. Methods for using the bleaching compositions are also provided.
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