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公开(公告)号：EP1025239B1
公开(公告)日：2008-01-23
申请号：EP98953936.6
申请日：1998-10-23
发明人： SCHELLENBERGER, Volker , KELLIS, James, T., Jr. , PAECH, Christian , NADHERNY, Joanne , NAKI, Donald, P. , POULOSE, Ayrookaran, J. , COLLIER, Katherine, D. , CALDWELL, Robert, M. , BAECK, André, C.
CPC分类号： C12N9/54 , A23G4/123 , A23K20/189 , A61K8/66 , A61K2800/86 , A61Q11/00 , A61Q19/00 , C11D3/386 , C11D3/38609 , C11D3/38618 , C11D3/3907 , C11D3/3945 , C12Y304/00 , C12Y304/21062
摘要： Novel protease variants derived from the DNA sequences of naturally-occurring or recombinant non-human proteases are disclosed. The variant proteases, in general, are obtained by in vitro modification of a precursor DNA sequence encoding the naturally-occurring or recombinant protease to generate the substitution of a plurality of amino acid residues in the amino acid sequence of a precursor protease. Such variant proteases have properties which are different from those of the precursor protease, such as altered wash performance. The substituted amino acid residue correspond to positions 62, 212, 230, 232, 252 and 257 of Bacillus amyloliquefaciens subtilisin.
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公开(公告)号：EP1027418A1
公开(公告)日：2000-08-16
申请号：EP98955109.8
申请日：1998-10-23
发明人： GHOSH, Chanchal, Kumar , BAECK, Andre, Cesar , OHTANI, Ryohei , BUSCH, Alfred , SHOWELL, Michael, Stanford , SCHELLENBERGER, Volker , KELLIS, James, T., Jr. , PAECH, Christian , NADHERNY, Joanne , NAKI, Donald, P. , COLLIER, Katherine, D. , CALDWELL, Robert, M. , POULOSE, Ayrookaran, J.
CPC分类号： C12N9/54 , A23G4/123 , A23K20/189 , A61K8/66 , A61K2800/86 , A61Q11/00 , A61Q19/00 , C11D3/386 , C11D3/38609 , C11D3/38618 , C11D3/3907 , C11D3/3945 , C12Y304/00 , C12Y304/21062
摘要： The present invention relates to cleaning compositions comprising a protease variant. One cleaning composition comprises a protease variant including a substitution of an amino acid residue with another naturally occurring amino acid residue at an amino acid residue position corresponding to position 103 of Bacillus amyloliquefaciens subtilisin in combination with a substitution of an amino acid residue with another naturally occurring amino acid residue at one or more amino acid residue positions corresponding to positions 1, 3, 4, 8, 9, 10, 12, 13, 16, 17, 18, 19, 20, 21, 22, 24, 27, 33, 37, 38, 42, 43, 48, 55, 57, 58, 61, 62, 68, 72, 75, 76, 77, 78, 79, 86, 87, 89, 97, 98, 99, 101, 102, 104, 106, 107, 109, 111, 114, 116, 117, 119, 121, 123, 126, 128, 130, 131, 133, 134, 137, 140, 141, 142, 146, 147, 158, 159, 160, 166, 167, 170, 173, 174, 177, 181, 182, 183, 184, 185, 188, 192, 194, 198, 203, 204, 205, 206, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 222, 224, 227, 228, 230, 232, 236, 237, 238, 240, 242, 243, 244, 245, 246, 247, 248, 249, 251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 265, 268, 269, 270, 271, 272, 274 and 275 of Bacillus amyloliquefaciens subtilisin; wherein when said protease variant includes a substitution of amino acid residues positions corresponding to positions 103 and 76, there is also a substitution of an amino acid residue at one or more amino acid residue positions other than amino acid residue positions corresponding to positions 27, 99, 101, 104, 107, 109, 123, 128, 166, 204, 206, 210, 216, 217, 218, 222, 260, 265 or 274 of Bacillus amyloliquefaciens subtilisin; and one or more cleaning adjunct materials. Another cleaning composition comprises a protease variant including a substitution of an amino acid residue with another naturally occurring amino acid residue at one or more amino acid residue positions corresponding to positions 62, 212, 230, 232, 252 and 257 of $(Bacillus amyloliquefaciens) subtilisin; and one or more cleaning adjunct materials. Methods for using the cleaning compositions are also provided.
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公开(公告)号：EP1025239A2
公开(公告)日：2000-08-09
申请号：EP98953936.6
申请日：1998-10-23
发明人： SCHELLENBERGER, Volker , KELLIS, James, T., Jr. , PAECH, Christian , NADHERNY, Joanne , NAKI, Donald, P. , POULOSE, Ayrookaran, J. , COLLIER, Katherine, D. , CALDWELL, Robert, M. , BAECK, André, C.
CPC分类号： C12N9/54 , A23G4/123 , A23K20/189 , A61K8/66 , A61K2800/86 , A61Q11/00 , A61Q19/00 , C11D3/386 , C11D3/38609 , C11D3/38618 , C11D3/3907 , C11D3/3945 , C12Y304/00 , C12Y304/21062
摘要： Novel protease variants derived from the DNA sequences of naturally-occurring or recombinant non-human proteases are disclosed. The variant proteases, in general, are obtained by in vitro modification of a precursor DNA sequence encoding the naturally-occurring or recombinant protease to generate the substitution of a plurality of amino acid residues in the amino acid sequence of a precursor protease. Such variant proteases have properties which are different from those of the precursor protease, such as altered wash performance. The substituted amino acid residue correspond to positions 62, 212, 230, 232, 252 and 257 of Bacillus amyloliquefaciens subtilisin.
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公开(公告)号：EP1025241B1
公开(公告)日：2005-03-30
申请号：EP98956181.6
申请日：1998-10-23
发明人： SCHELLENBERGER, Volker , KELLIS, James, T., Jr. , PAECH, Christian , NADHERNY, Joanne , NAKI, Donald, P. , POULOSE, Ayrookaran, J. , COLLIER, Katherine, D. , CALDWELL, Robert, M. , BAECK, André, C.
CPC分类号： C12N9/54 , A23G4/123 , A23K20/189 , A61K8/66 , A61K2800/86 , A61Q11/00 , A61Q19/00 , C11D3/386 , C11D3/38609 , C11D3/38618 , C11D3/3907 , C11D3/3945 , C12Y304/00 , C12Y304/21062
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公开(公告)号：EP1025241B2
公开(公告)日：2009-10-21
申请号：EP98956181.6
申请日：1998-10-23
发明人： SCHELLENBERGER, Volker , KELLIS, James, T., Jr. , PAECH, Christian , NADHERNY, Joanne , NAKI, Donald, P. , POULOSE, Ayrookaran, J. , COLLIER, Katherine, D. , CALDWELL, Robert, M. , BAECK, André, C.
CPC分类号： C12N9/54 , A23G4/123 , A23K20/189 , A61K8/66 , A61K2800/86 , A61Q11/00 , A61Q19/00 , C11D3/386 , C11D3/38609 , C11D3/38618 , C11D3/3907 , C11D3/3945 , C12Y304/00 , C12Y304/21062
摘要： Novel protease variants derived from the DNA sequences of naturally-occurring or recombinant non-human proteases are disclosed. The variant proteases, in general, are obtained by in vitro modification of a precursor DNA sequence encoding the naturally-occurring or recombinant protease to generate the substitution of a plurality of amino acid residues in the amino acid sequence of a precursor protease. Such variant proteases have properties which are different from those of the precursor protease, such as altered wash performance. The substituted amino acid residue corresponds to position 103 in combination with one or more of the following substitutions at residue positions corresponding to positions 1, 3, 4, 8, 10, 12, 13, 15, 16, 17, 18, 19, 20, 21, 22, 24, 27, 33, 37, 38, 42, 43, 48, 55, 57, 58, 61, 62, 68, 72, 75, 76, 77, 78, 79, 86, 87, 89, 97, 98, 99, 101, 102, 104, 106, 107, 109, 111, 114, 116, 117, 119, 121, 123, 126, 128, 130, 131, 133, 134, 137, 140, 141, 142, 146, 147, 158, 159, 160, 166, 167, 170, 173, 174, 177, 181, 182, 183, 184, 185, 188, 192, 194, 198, 203, 204, 205, 206, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 222, 224, 227, 228, 230, 232, 236, 237, 238, 240, 242, 243, 244, 245, 246, 247, 248, 249, 251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 265, 268, 269, 270, 271, 272, 274, and 275 of Bacillus amyloliquefaciens subtilisin, wherein when a substitution at a position corresponding to residue position 103 is combined with a substitution at a position corresponding to residue position 76, there is also a substitution at one or more residue positions other than residue positions corresponding to positions 27, 99, 101, 104, 107, 109, 123, 128, 166, 204, 206, 210, 216, 217, 218, 222, 260, 265 or 274 of Bacillus amyloliquefaciens subtilisin.
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公开(公告)号：EP1025241A2
公开(公告)日：2000-08-09
申请号：EP98956181.6
申请日：1998-10-23
发明人： SCHELLENBERGER, Volker , KELLIS, James, T., Jr. , PAECH, Christian , NADHERNY, Joanne , NAKI, Donald, P. , POULOSE, Ayrookaran, J. , COLLIER, Katherine, D. , CALDWELL, Robert, M. , BAECK, André, C.
CPC分类号： C12N9/54 , A23G4/123 , A23K20/189 , A61K8/66 , A61K2800/86 , A61Q11/00 , A61Q19/00 , C11D3/386 , C11D3/38609 , C11D3/38618 , C11D3/3907 , C11D3/3945 , C12Y304/00 , C12Y304/21062
摘要： Novel protease variants derived from the DNA sequences of naturally-occurring or recombinant non-human proteases are disclosed. The variant proteases, in general, are obtained by in vitro modification of a precursor DNA sequence encoding the naturally-occurring or recombinant protease to generate the substitution of a plurality of amino acid residues in the amino acid sequence of a precursor protease. Such variant proteases have properties which are different from those of the precursor protease, such as altered wash performance. The substituted amino acid residue corresponds to position 103 in combination with one or more of the following substitutions at residue positions corresponding to positions 1, 3, 4, 8, 10, 12, 13, 15, 16, 17, 18, 19, 20, 21, 22, 24, 27, 33, 37, 38, 42, 43, 48, 55, 57, 58, 61, 62, 68, 72, 75, 76, 77, 78, 79, 86, 87, 89, 97, 98, 99, 101, 102, 104, 106, 107, 109, 111, 114, 116, 117, 119, 121, 123, 126, 128, 130, 131, 133, 134, 137, 140, 141, 142, 146, 147, 158, 159, 160, 166, 167, 170, 173, 174, 177, 181, 182, 183, 184, 185, 188, 192, 194, 198, 203, 204, 205, 206, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 222, 224, 227, 228, 230, 232, 236, 237, 238, 240, 242, 243, 244, 245, 246, 247, 248, 249, 251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 265, 268, 269, 270, 271, 272, 274, and 275 of Bacillus amyloliquefaciens subtilisin, wherein when a substitution at a position corresponding to residue position 103 is combined with a substitution at a position corresponding to residue position 76, there is also a substitution at one or more residue positions other than residue positions corresponding to positions 27, 99, 101, 104, 107, 109, 123, 128, 166, 204, 206, 210, 216, 217, 218, 222, 260, 265 or 274 of Bacillus amyloliquefaciens subtilisin.
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7.发明公开
公开(公告)号：EP1025194A1
公开(公告)日：2000-08-09
申请号：EP98955079.3
申请日：1998-10-23
发明人： GHOSH, Chanchal, Kumar , BAECK, Andre, Cesar , OHTANI, Ryohei , BUSCH, Alfred , SHOWELL, Michael, Stanford , SCHELLENBERGER, Volker , KELLIS, James, T., Jr. , PAECH, Christian , NADHERNY, Joanne , NAKI, Donald, P. , COLLIER, Katherine, D. , CALDWELL, Robert, M. , POULOSE, Ayrookaran, J.
CPC分类号： C12N9/54 , A23G4/123 , A23K20/189 , A61K8/66 , A61K2800/86 , A61Q11/00 , A61Q19/00 , C11D3/386 , C11D3/38609 , C11D3/38618 , C11D3/3907 , C11D3/3945 , C12Y304/00 , C12Y304/21062
摘要： The present invention relates to bleaching compositions comprising a protease variant. One bleaching composition comprises a protease variant including a substitution of an amino acid residue with another naturally occurring amino acid residue at an amino acid residue position corresponding to position 103 of Bacillus amyloliquefaciens subtilisin in combination with a substitution of an amino acid residue with another naturally occurring amino acid residue at one or more amino acid residue positions corresponding to positions 1, 3, 4, 8, 9, 10, 12, 13, 16, 17, 18, 19, 20, 21, 22, 24, 27, 33, 37, 38, 42, 43, 48, 55, 57, 58, 61, 62, 68, 72, 75, 76, 77, 78, 79, 86, 87, 89, 97, 98, 99, 101, 102, 104, 106, 107, 109, 111, 114, 116, 117, 119, 121, 123, 126, 128, 130, 131, 133, 134, 137, 140, 141, 142, 146, 147, 158, 159, 160, 166, 167, 170, 173, 174, 177, 181, 182, 183, 184, 185, 188, 192, 194, 198, 203, 204, 205, 206, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 222, 224, 227, 228, 230, 232, 236, 237, 238, 240, 242, 243, 244, 245, 246, 247, 248, 249, 251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 265, 268, 269, 270, 271, 272, 274, and 275 of Bacillus amyloliquefaciens subtilisin; wherein when said protease variant includes a substitution of amino acid residues at positions corresponding to positions 103 and 76, there is also a substitution of an amino acid residue at one or more amino acid residue positions other than amino acid residue positions corresponding to positions 27, 99, 101, 104, 107, 109, 123, 128, 166, 204, 206, 210, 216, 217, 218, 222, 260, 265, or 274 of Bacillus amyloliquefaciens subtilisin, a bleaching agent; and one or more cleaning adjunct materials. Another bleaching composition comprises a protease variant including a substitution of an amino acid residue with another naturally occurring amino acid residue at one or more amino acid residue positions corresponding to positions 62, 212, 230, 232, 252 and 257 of Bacillus amyloliquefaciens subtilisin; a bleaching agent; and one or more cleaning adjunct materials. Methods for using the bleaching compositions are also provided.
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公开(公告)号：EP3868881A3
公开(公告)日：2021-11-03
申请号：EP20213383.1
申请日：2011-05-05
发明人： ALEKSEYEV, Viktor Yuryevich , AMIN, Neelam, S , AUGUSTYN, Katherine , CASCAO-PEREIRA, Luis Gustavo , COLLIER, Katherine, D. , ESTELL, David, A. , KELLIS JR., James, T , POULOSE, Ayrookaran, J. , SOUTER, Philip Frank , WARD, Glenn Steven , YAO, Jian
摘要： The present invention provides isolated subtilisin variants. Specifically, a subtilisin variant is a mature form having proteolytic activity and comprises an amino acid sequence comprising the combination of amino acid substitutions: T022R, S101G, S103A, V104I, A232V, and Q245R, wherein the variant is a variant of Bacillus lentus subtilisin GG36 protease comprising the amino acid sequence shown in SEQ ID NO:2, and wherein the variant has at least 90% amino acid sequence identity to the amino acid sequence of SEQ ID NO: 2, and wherein the amino acid positions of the subtilisin variant are numbered according to the numbering of corresponding amino acid positions in the amino acid sequence of B. amyloliquefaciens subtilisin BPN' set forth as SEQ ID NO: 1. In addition, the present invention provides compositions comprising these subtilisin variants. In some embodiments, the present invention provides cleaning compositions comprising at least one of these subtilisin variants and methods of cleaning using the cleaning compositions.
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公开(公告)号：EP3868881A2
公开(公告)日：2021-08-25
申请号：EP20213383.1
申请日：2011-05-05
发明人： ALEKSEYEV, Viktor Yuryevich , AMIN, Neelam, S , AUGUSTYN, Katherine , CASCAO-PEREIRA, Luis Gustavo , COLLIER, Katherine, D. , ESTELL, David, A. , KELLIS JR., James, T , POULOSE, Ayrookaran, J. , SOUTER, Philip Frank , WARD, Glenn Steven , YAO, Jian
摘要： The present invention provides isolated subtilisin variants. Specifically, a subtilisin variant is a mature form having proteolytic activity and comprises an amino acid sequence comprising the combination of amino acid substitutions: T022R, S101G, S103A, V104I, A232V, and Q245R, wherein the variant is a variant of Bacillus lentus subtilisin GG36 protease comprising the amino acid sequence shown in SEQ ID NO:2, and wherein the variant has at least 90% amino acid sequence identity to the amino acid sequence of SEQ ID NO: 2, and wherein the amino acid positions of the subtilisin variant are numbered according to the numbering of corresponding amino acid positions in the amino acid sequence of B. amyloliquefaciens subtilisin BPN' set forth as SEQ ID NO: 1. In addition, the present invention provides compositions comprising these subtilisin variants. In some embodiments, the present invention provides cleaning compositions comprising at least one of these subtilisin variants and methods of cleaning using the cleaning compositions.
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公开(公告)号：EP2566960B1
公开(公告)日：2017-03-08
申请号：EP11720664.9
申请日：2011-05-05
发明人： SOUTER, Phillip, Frank , WARD, Glenn, Steven , POULOSE, Ayrookaran, Joseph , ESTELL, David, A. , KELLIS, James, T., Jr. , COLLIER, Katherine, D. , CASCÃO-PEREIRA, Luis, Gustavo , ALEKSEYEV, Viktor, Yuryevich , AMIN, Neelam, S. , YAO, Jian , AUGUSTYN, Katherine
CPC分类号： C12N9/54 , C11D3/386 , C11D3/38681
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