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公开(公告)号：EP1025239A2
公开(公告)日：2000-08-09
申请号：EP98953936.6
申请日：1998-10-23
发明人： SCHELLENBERGER, Volker , KELLIS, James, T., Jr. , PAECH, Christian , NADHERNY, Joanne , NAKI, Donald, P. , POULOSE, Ayrookaran, J. , COLLIER, Katherine, D. , CALDWELL, Robert, M. , BAECK, André, C.
CPC分类号： C12N9/54 , A23G4/123 , A23K20/189 , A61K8/66 , A61K2800/86 , A61Q11/00 , A61Q19/00 , C11D3/386 , C11D3/38609 , C11D3/38618 , C11D3/3907 , C11D3/3945 , C12Y304/00 , C12Y304/21062
摘要： Novel protease variants derived from the DNA sequences of naturally-occurring or recombinant non-human proteases are disclosed. The variant proteases, in general, are obtained by in vitro modification of a precursor DNA sequence encoding the naturally-occurring or recombinant protease to generate the substitution of a plurality of amino acid residues in the amino acid sequence of a precursor protease. Such variant proteases have properties which are different from those of the precursor protease, such as altered wash performance. The substituted amino acid residue correspond to positions 62, 212, 230, 232, 252 and 257 of Bacillus amyloliquefaciens subtilisin.
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公开(公告)号：EP1025241B1
公开(公告)日：2005-03-30
申请号：EP98956181.6
申请日：1998-10-23
发明人： SCHELLENBERGER, Volker , KELLIS, James, T., Jr. , PAECH, Christian , NADHERNY, Joanne , NAKI, Donald, P. , POULOSE, Ayrookaran, J. , COLLIER, Katherine, D. , CALDWELL, Robert, M. , BAECK, André, C.
CPC分类号： C12N9/54 , A23G4/123 , A23K20/189 , A61K8/66 , A61K2800/86 , A61Q11/00 , A61Q19/00 , C11D3/386 , C11D3/38609 , C11D3/38618 , C11D3/3907 , C11D3/3945 , C12Y304/00 , C12Y304/21062
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公开(公告)号：EP1025239B1
公开(公告)日：2008-01-23
申请号：EP98953936.6
申请日：1998-10-23
发明人： SCHELLENBERGER, Volker , KELLIS, James, T., Jr. , PAECH, Christian , NADHERNY, Joanne , NAKI, Donald, P. , POULOSE, Ayrookaran, J. , COLLIER, Katherine, D. , CALDWELL, Robert, M. , BAECK, André, C.
CPC分类号： C12N9/54 , A23G4/123 , A23K20/189 , A61K8/66 , A61K2800/86 , A61Q11/00 , A61Q19/00 , C11D3/386 , C11D3/38609 , C11D3/38618 , C11D3/3907 , C11D3/3945 , C12Y304/00 , C12Y304/21062
摘要： Novel protease variants derived from the DNA sequences of naturally-occurring or recombinant non-human proteases are disclosed. The variant proteases, in general, are obtained by in vitro modification of a precursor DNA sequence encoding the naturally-occurring or recombinant protease to generate the substitution of a plurality of amino acid residues in the amino acid sequence of a precursor protease. Such variant proteases have properties which are different from those of the precursor protease, such as altered wash performance. The substituted amino acid residue correspond to positions 62, 212, 230, 232, 252 and 257 of Bacillus amyloliquefaciens subtilisin.
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公开(公告)号：EP1027418A1
公开(公告)日：2000-08-16
申请号：EP98955109.8
申请日：1998-10-23
发明人： GHOSH, Chanchal, Kumar , BAECK, Andre, Cesar , OHTANI, Ryohei , BUSCH, Alfred , SHOWELL, Michael, Stanford , SCHELLENBERGER, Volker , KELLIS, James, T., Jr. , PAECH, Christian , NADHERNY, Joanne , NAKI, Donald, P. , COLLIER, Katherine, D. , CALDWELL, Robert, M. , POULOSE, Ayrookaran, J.
CPC分类号： C12N9/54 , A23G4/123 , A23K20/189 , A61K8/66 , A61K2800/86 , A61Q11/00 , A61Q19/00 , C11D3/386 , C11D3/38609 , C11D3/38618 , C11D3/3907 , C11D3/3945 , C12Y304/00 , C12Y304/21062
摘要： The present invention relates to cleaning compositions comprising a protease variant. One cleaning composition comprises a protease variant including a substitution of an amino acid residue with another naturally occurring amino acid residue at an amino acid residue position corresponding to position 103 of Bacillus amyloliquefaciens subtilisin in combination with a substitution of an amino acid residue with another naturally occurring amino acid residue at one or more amino acid residue positions corresponding to positions 1, 3, 4, 8, 9, 10, 12, 13, 16, 17, 18, 19, 20, 21, 22, 24, 27, 33, 37, 38, 42, 43, 48, 55, 57, 58, 61, 62, 68, 72, 75, 76, 77, 78, 79, 86, 87, 89, 97, 98, 99, 101, 102, 104, 106, 107, 109, 111, 114, 116, 117, 119, 121, 123, 126, 128, 130, 131, 133, 134, 137, 140, 141, 142, 146, 147, 158, 159, 160, 166, 167, 170, 173, 174, 177, 181, 182, 183, 184, 185, 188, 192, 194, 198, 203, 204, 205, 206, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 222, 224, 227, 228, 230, 232, 236, 237, 238, 240, 242, 243, 244, 245, 246, 247, 248, 249, 251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 265, 268, 269, 270, 271, 272, 274 and 275 of Bacillus amyloliquefaciens subtilisin; wherein when said protease variant includes a substitution of amino acid residues positions corresponding to positions 103 and 76, there is also a substitution of an amino acid residue at one or more amino acid residue positions other than amino acid residue positions corresponding to positions 27, 99, 101, 104, 107, 109, 123, 128, 166, 204, 206, 210, 216, 217, 218, 222, 260, 265 or 274 of Bacillus amyloliquefaciens subtilisin; and one or more cleaning adjunct materials. Another cleaning composition comprises a protease variant including a substitution of an amino acid residue with another naturally occurring amino acid residue at one or more amino acid residue positions corresponding to positions 62, 212, 230, 232, 252 and 257 of $(Bacillus amyloliquefaciens) subtilisin; and one or more cleaning adjunct materials. Methods for using the cleaning compositions are also provided.
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5.发明公开
公开(公告)号：EP1025194A1
公开(公告)日：2000-08-09
申请号：EP98955079.3
申请日：1998-10-23
发明人： GHOSH, Chanchal, Kumar , BAECK, Andre, Cesar , OHTANI, Ryohei , BUSCH, Alfred , SHOWELL, Michael, Stanford , SCHELLENBERGER, Volker , KELLIS, James, T., Jr. , PAECH, Christian , NADHERNY, Joanne , NAKI, Donald, P. , COLLIER, Katherine, D. , CALDWELL, Robert, M. , POULOSE, Ayrookaran, J.
CPC分类号： C12N9/54 , A23G4/123 , A23K20/189 , A61K8/66 , A61K2800/86 , A61Q11/00 , A61Q19/00 , C11D3/386 , C11D3/38609 , C11D3/38618 , C11D3/3907 , C11D3/3945 , C12Y304/00 , C12Y304/21062
摘要： The present invention relates to bleaching compositions comprising a protease variant. One bleaching composition comprises a protease variant including a substitution of an amino acid residue with another naturally occurring amino acid residue at an amino acid residue position corresponding to position 103 of Bacillus amyloliquefaciens subtilisin in combination with a substitution of an amino acid residue with another naturally occurring amino acid residue at one or more amino acid residue positions corresponding to positions 1, 3, 4, 8, 9, 10, 12, 13, 16, 17, 18, 19, 20, 21, 22, 24, 27, 33, 37, 38, 42, 43, 48, 55, 57, 58, 61, 62, 68, 72, 75, 76, 77, 78, 79, 86, 87, 89, 97, 98, 99, 101, 102, 104, 106, 107, 109, 111, 114, 116, 117, 119, 121, 123, 126, 128, 130, 131, 133, 134, 137, 140, 141, 142, 146, 147, 158, 159, 160, 166, 167, 170, 173, 174, 177, 181, 182, 183, 184, 185, 188, 192, 194, 198, 203, 204, 205, 206, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 222, 224, 227, 228, 230, 232, 236, 237, 238, 240, 242, 243, 244, 245, 246, 247, 248, 249, 251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 265, 268, 269, 270, 271, 272, 274, and 275 of Bacillus amyloliquefaciens subtilisin; wherein when said protease variant includes a substitution of amino acid residues at positions corresponding to positions 103 and 76, there is also a substitution of an amino acid residue at one or more amino acid residue positions other than amino acid residue positions corresponding to positions 27, 99, 101, 104, 107, 109, 123, 128, 166, 204, 206, 210, 216, 217, 218, 222, 260, 265, or 274 of Bacillus amyloliquefaciens subtilisin, a bleaching agent; and one or more cleaning adjunct materials. Another bleaching composition comprises a protease variant including a substitution of an amino acid residue with another naturally occurring amino acid residue at one or more amino acid residue positions corresponding to positions 62, 212, 230, 232, 252 and 257 of Bacillus amyloliquefaciens subtilisin; a bleaching agent; and one or more cleaning adjunct materials. Methods for using the bleaching compositions are also provided.
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公开(公告)号：EP1025241B2
公开(公告)日：2009-10-21
申请号：EP98956181.6
申请日：1998-10-23
发明人： SCHELLENBERGER, Volker , KELLIS, James, T., Jr. , PAECH, Christian , NADHERNY, Joanne , NAKI, Donald, P. , POULOSE, Ayrookaran, J. , COLLIER, Katherine, D. , CALDWELL, Robert, M. , BAECK, André, C.
CPC分类号： C12N9/54 , A23G4/123 , A23K20/189 , A61K8/66 , A61K2800/86 , A61Q11/00 , A61Q19/00 , C11D3/386 , C11D3/38609 , C11D3/38618 , C11D3/3907 , C11D3/3945 , C12Y304/00 , C12Y304/21062
摘要： Novel protease variants derived from the DNA sequences of naturally-occurring or recombinant non-human proteases are disclosed. The variant proteases, in general, are obtained by in vitro modification of a precursor DNA sequence encoding the naturally-occurring or recombinant protease to generate the substitution of a plurality of amino acid residues in the amino acid sequence of a precursor protease. Such variant proteases have properties which are different from those of the precursor protease, such as altered wash performance. The substituted amino acid residue corresponds to position 103 in combination with one or more of the following substitutions at residue positions corresponding to positions 1, 3, 4, 8, 10, 12, 13, 15, 16, 17, 18, 19, 20, 21, 22, 24, 27, 33, 37, 38, 42, 43, 48, 55, 57, 58, 61, 62, 68, 72, 75, 76, 77, 78, 79, 86, 87, 89, 97, 98, 99, 101, 102, 104, 106, 107, 109, 111, 114, 116, 117, 119, 121, 123, 126, 128, 130, 131, 133, 134, 137, 140, 141, 142, 146, 147, 158, 159, 160, 166, 167, 170, 173, 174, 177, 181, 182, 183, 184, 185, 188, 192, 194, 198, 203, 204, 205, 206, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 222, 224, 227, 228, 230, 232, 236, 237, 238, 240, 242, 243, 244, 245, 246, 247, 248, 249, 251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 265, 268, 269, 270, 271, 272, 274, and 275 of Bacillus amyloliquefaciens subtilisin, wherein when a substitution at a position corresponding to residue position 103 is combined with a substitution at a position corresponding to residue position 76, there is also a substitution at one or more residue positions other than residue positions corresponding to positions 27, 99, 101, 104, 107, 109, 123, 128, 166, 204, 206, 210, 216, 217, 218, 222, 260, 265 or 274 of Bacillus amyloliquefaciens subtilisin.
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公开(公告)号：EP1025241A2
公开(公告)日：2000-08-09
申请号：EP98956181.6
申请日：1998-10-23
发明人： SCHELLENBERGER, Volker , KELLIS, James, T., Jr. , PAECH, Christian , NADHERNY, Joanne , NAKI, Donald, P. , POULOSE, Ayrookaran, J. , COLLIER, Katherine, D. , CALDWELL, Robert, M. , BAECK, André, C.
CPC分类号： C12N9/54 , A23G4/123 , A23K20/189 , A61K8/66 , A61K2800/86 , A61Q11/00 , A61Q19/00 , C11D3/386 , C11D3/38609 , C11D3/38618 , C11D3/3907 , C11D3/3945 , C12Y304/00 , C12Y304/21062
摘要： Novel protease variants derived from the DNA sequences of naturally-occurring or recombinant non-human proteases are disclosed. The variant proteases, in general, are obtained by in vitro modification of a precursor DNA sequence encoding the naturally-occurring or recombinant protease to generate the substitution of a plurality of amino acid residues in the amino acid sequence of a precursor protease. Such variant proteases have properties which are different from those of the precursor protease, such as altered wash performance. The substituted amino acid residue corresponds to position 103 in combination with one or more of the following substitutions at residue positions corresponding to positions 1, 3, 4, 8, 10, 12, 13, 15, 16, 17, 18, 19, 20, 21, 22, 24, 27, 33, 37, 38, 42, 43, 48, 55, 57, 58, 61, 62, 68, 72, 75, 76, 77, 78, 79, 86, 87, 89, 97, 98, 99, 101, 102, 104, 106, 107, 109, 111, 114, 116, 117, 119, 121, 123, 126, 128, 130, 131, 133, 134, 137, 140, 141, 142, 146, 147, 158, 159, 160, 166, 167, 170, 173, 174, 177, 181, 182, 183, 184, 185, 188, 192, 194, 198, 203, 204, 205, 206, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 222, 224, 227, 228, 230, 232, 236, 237, 238, 240, 242, 243, 244, 245, 246, 247, 248, 249, 251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 265, 268, 269, 270, 271, 272, 274, and 275 of Bacillus amyloliquefaciens subtilisin, wherein when a substitution at a position corresponding to residue position 103 is combined with a substitution at a position corresponding to residue position 76, there is also a substitution at one or more residue positions other than residue positions corresponding to positions 27, 99, 101, 104, 107, 109, 123, 128, 166, 204, 206, 210, 216, 217, 218, 222, 260, 265 or 274 of Bacillus amyloliquefaciens subtilisin.
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公开(公告)号：EP2566960B1
公开(公告)日：2017-03-08
申请号：EP11720664.9
申请日：2011-05-05
发明人： SOUTER, Phillip, Frank , WARD, Glenn, Steven , POULOSE, Ayrookaran, Joseph , ESTELL, David, A. , KELLIS, James, T., Jr. , COLLIER, Katherine, D. , CASCÃO-PEREIRA, Luis, Gustavo , ALEKSEYEV, Viktor, Yuryevich , AMIN, Neelam, S. , YAO, Jian , AUGUSTYN, Katherine
CPC分类号： C12N9/54 , C11D3/386 , C11D3/38681
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公开(公告)号：EP3434764A3
公开(公告)日：2019-04-03
申请号：EP18181021.9
申请日：2010-12-09
发明人： SOUTER, Philip Frank , WARD, Glenn Steven , BASLER, Jashua Roy , CASCAO-PEREIRA, Luis Gustavo , ESTELL, David, A. , KELLIS, James, T., Jr. , PISARCHIK, Alexander , POULOUSE, Ayrookaran, J. , TORRES-PAZMINO, Daniel , ALEKSEYEV, Viktor, Yuryevich
摘要： This invention relates to fabric and home care products comprising one or more cold water proteases and processes for making and using such products. Such compositions provide improved cleaning and freshness. Such cold water proteases may be derived from parent enzymes, including BPN' subtilisin and subtilisin derived from Bacillus lentus, by substitution, insertion and/or deletion of one or more of the parent enzymes' amino acids.
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公开(公告)号：EP3434764A2
公开(公告)日：2019-01-30
申请号：EP18181021.9
申请日：2010-12-09
发明人： SOUTER, Philip Frank , WARD, Glenn Steven , BASLER, Jashua Roy , CASCAO-PEREIRA, Luis Gustavo , ESTELL, David, A. , KELLIS, James, T., Jr. , PISARCHIK, Alexander , POULOUSE, Ayrookaran, J. , TORRES-PAZMINO, Daniel , ALEKSEYEV, Viktor, Yuryevich
摘要： This invention relates to fabric and home care products comprising one or more cold water proteases and processes for making and using such products. Such compositions provide improved cleaning and freshness. Such cold water proteases may be derived from parent enzymes, including BPN' subtilisin and subtilisin derived from Bacillus lentus, by substitution, insertion and/or deletion of one or more of the parent enzymes' amino acids.
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