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公开(公告)号:EP0258067B1
公开(公告)日:1993-03-24
申请号:EP87307668.1
申请日:1987-08-28
CPC分类号: C12N15/81 , C07K14/765
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公开(公告)号:EP0258067A3
公开(公告)日:1989-05-10
申请号:EP87307668.1
申请日:1987-08-28
CPC分类号: C12N15/81 , C07K14/765
摘要: A hybrid yeast promoter comprising constituents of the 5ʹ non-coding PGK region and, as an upstream activation sequence, the upstream activation sequence of the GAL 10 gene of Saccharomyces cerevisiae , and which does not contain the endogenous PGK upstream activation sequence. Preferably the GAL 10 upstream activation sequence is provided at a site from which the PGK upstream activation sequence has been deleted. The hybrid promoter confers galactose regulation of gene transcription.
摘要翻译: 杂交酵母启动子包含5'非编码PGK区域的组成部分,和作为上游激活序列的酿酒酵母GAL10基因的上游激活序列,并且其不含内源PGK上游激活序列。 优选地,GAL10上游激活序列在PGK上游激活序列已经从其中缺失的位点提供。 杂合启动子赋予半乳糖调节基因转录。
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公开(公告)号:EP0258067A2
公开(公告)日:1988-03-02
申请号:EP87307668.1
申请日:1987-08-28
CPC分类号: C12N15/81 , C07K14/765
摘要: A hybrid yeast promoter comprising constituents of the 5ʹ non-coding PGK region and, as an upstream activation sequence, the upstream activation sequence of the GAL 10 gene of Saccharomyces cerevisiae , and which does not contain the endogenous PGK upstream activation sequence. Preferably the GAL 10 upstream activation sequence is provided at a site from which the PGK upstream activation sequence has been deleted. The hybrid promoter confers galactose regulation of gene transcription.
摘要翻译: 一种杂合酵母启动子,其包含5'非编码PGK区的组成,并且作为上游活化序列,是酿酒酵母的GAL10基因的上游活化序列,并且不包含内源性PGK上游激活序列。 优选地,在缺失PGK上游激活序列的位点处提供GAL10上游激活序列。 杂交启动子赋予基因转录的半乳糖调节。
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