公开(公告)号：EP3372522A1

公开(公告)日：2018-09-12

申请号：EP16862103.5

申请日：2016-11-02

申请人： Eiken Kagaku Kabushiki Kaisha

发明人： SHINDOME, Tsuyoshi ,  NATSUME, Waka

IPC分类号： B65D47/18 ,  B01D63/08 ,  G01N1/10 ,  B01D39/16 ,  C12M1/00

CPC分类号： B01D63/08 ,  B01D39/16 ,  B01D39/1676 ,  B01D69/02 ,  B01D2239/1208 ,  B01D2239/1216 ,  B01D2325/36 ,  B05B11/047 ,  B65D47/18 ,  C12M1/00 ,  G01N1/10 ,  G01N1/14 ,  G01N1/4077 ,  G01N2001/4088

摘要： To provide a discharge member with a filter which enables a filtrate to be dripped into a dripping container while filtering a suspension, enables an excessive portion to be suctioned when the dripped amount is excessive, thereby to allow a fixed amount of a filtrate to be injected into the dripping container, and thus enables suctioning operation of at the time of suctioning an excessive portion to be conducted preferably without deteriorating its dripping performance and filtering performance, a filter F provided in the discharge member 4 with a filter is a composite filter in which a pre-filter PF comprising a continuous porous foam that is softened at least in a wet state is arrange on the filtration surface side of a membrane filter MF.

公开(公告)号：EP2218791B1

公开(公告)日：2015-01-07

申请号：EP08847811.0

申请日：2008-11-05

申请人： EIKEN KAGAKU KABUSHIKI KAISHA

发明人： MORI, Yasuyoshi ,  NOTOMI, Tsugunori ,  SHINDOME, Tsuyoshi

IPC分类号： C12Q1/68 ,  C12N15/10

CPC分类号： C12N15/1006

公开(公告)号：EP2218791A1

公开(公告)日：2010-08-18

申请号：EP08847811.0

申请日：2008-11-05

申请人： EIKEN KAGAKU KABUSHIKI KAISHA

发明人： MORI, Yasuyoshi ,  NOTOMI, Tsugunori ,  SHINDOME, Tsuyoshi

IPC分类号： C12Q1/68 ,  C12N15/09

CPC分类号： C12N15/1006

摘要： The object is to remove substances that can inhibit nucleic acid amplification from biological samples and nucleic acid extracts, and to allow convenient and accurate evaluation of the presence or absence of a target nucleic acid or the expression level of a target gene in a biological sample, by nucleic acid amplification means employing an enzyme. The invention provides a method for preparation of a sample for use in nucleic acid amplification, to be used for amplification of nucleic acid in a biological sample, which method comprises an extraction step in which a nucleic acid extraction reagent is added to the biological sample to obtain a nucleic acid extract, and a purification step in which the nucleic acid extract is contacted with zeolite to obtain a nucleic acid amplification sample suitable for nucleic acid amplification, to allow nucleic acid detection at high sensitivity.

摘要翻译： 本发明的目的是去除能够抑制生物样品和核酸提取物的核酸扩增的物质，并且允许方便和准确地评估靶核酸的存在或不存在或生物样品中靶基因的表达水平， 通过使用酶的核酸扩增方法。 本发明提供了一种制备用于核酸扩增的样品的方法，用于生物样品中的核酸扩增，该方法包括提取步骤，其中将核酸提取试剂加入生物样品至 获得核酸提取物和纯化步骤，其中核酸提取物与沸石接触以获得适合于核酸扩增的核酸扩增样品，以允许高灵敏度的核酸检测。