公开(公告)号：EP3396430A1

公开(公告)日：2018-10-31

申请号：EP17400019.0

申请日：2017-04-27

申请人： Euroimmun Medizinische Labordiagnostika AG

发明人： Feirer, Christian ,  Roznowicz, Rafael ,  Paul, Marc ,  Bernitt, Erik

IPC分类号： G02B21/24 ,  G02B21/36

CPC分类号： G02B21/367 ,  G02B21/242 ,  G02B21/244 ,  G02B21/245

摘要： It is provided a method for optical scanning of at least one object region (105) placed on a transparent specimen holder (107), the method comprising: for each sample lateral position (x1) of plural predefined sample lateral positions (x0,x1,x2) performing a focus determination by: performing laser reflection and using a first camera (109) taking plural first images to determine a reference distance (rd) between the specimen holder (107) and an objective lens (113); performing transmission flash illumination and using a second camera (111) taking plural second images to define a focus distance taking into account the reference distance (rd); after completing the focus determination, determining a focus distance topology (600; 700) across the object region (105) based on the focus distances determined for all sample lateral positions (x0,x1,x2); and laterally moving the specimen holder (107) and acquiring third images while focusing according to the focus distance topology (600, 700);