摘要:
Disclosed are methods for diagnosing Bacterial Vaginosis in a subject comprising performing an assay for the detection of any one or more of Lactobacillus sp., Gardneralla vaginalis, and Eggerthella sp. in a subject sample. Also disclosed are methods and compositions for detecting Lactobacillus sp., Gardneralla vaginalis, and/or Eggerthella nucleic acid in a sample.
摘要:
There is provided a method for determining the presence or absence of Candida glabrata in a sample, the method comprising: (1) contacting a sample, said sample suspected of containing Candida sp., with a first amplification oligomer combination comprising, first and second C . glabrata -specific amplification oligomers for amplifying a Candida sp. target nucleic acid region, wherein said target region corresponds to a region of SEQ ID NO: 131 from about nucleotide position 355 to about nucleotide position 554, and wherein the first and second C . glabrata- specific amplification oligomers respectively comprise first and second C . glabrata- specific target-hybridizing sequences; and (2) performing an in vitro nucleic acid amplification reaction, wherein any Candida sp. target nucleic acid, if present in the sample, is used as a template for generating one or more amplification products corresponding to the target region; and (3) detecting the presence or absence of the one or more amplification products, thereby determining the presence or absence of Candida sp. in the sample.
摘要:
The disclosed invention include nucleic acid oligomers that may be used as amplification oligomers, including primers, as capture probes for sample preparation, and detection probes for detection of 16S rRNA from Atopobium vaginae in samples by using methods of specific nucleic acid amplification and detection.
摘要:
There is provided a method for determining the presence or absence of Candida glabrata in a sample, the method comprising: (1) contacting a sample, said sample suspected of containing Candida sp., with a first amplification oligomer combination comprising, first and second C . glabrata -specific amplification oligomers for amplifying a Candida sp. target nucleic acid region, wherein said target region corresponds to a region of SEQ ID NO: 131 from about nucleotide position 355 to about nucleotide position 554, and wherein the first and second C . glabrata- specific amplification oligomers respectively comprise first and second C . glabrata- specific target-hybridizing sequences; and (2) performing an in vitro nucleic acid amplification reaction, wherein any Candida sp. target nucleic acid, if present in the sample, is used as a template for generating one or more amplification products corresponding to the target region; and (3) detecting the presence or absence of the one or more amplification products, thereby determining the presence or absence of Candida sp. in the sample.
摘要:
Disclosed are methods utilizing specific amplification of Candida sp. target nucleic acid for detecting the presence or absence of Candida sp. in a sample. Also disclosed are corresponding oligomers, including amplification oligomers, capture probes and detection probes, and combinations thereof, as well as corresponding reaction mixtures and kits.