METHODS AND COMPOSITIONS FOR DETECTING CANDIDA SPECIES

    公开(公告)号:EP4249609A2

    公开(公告)日:2023-09-27

    申请号:EP23169555.2

    申请日:2017-01-04

    IPC分类号: C12Q1/6895

    摘要: There is provided a method for determining the presence or absence of Candida glabrata in a sample, the method comprising: (1) contacting a sample, said sample suspected of containing Candida sp., with a first amplification oligomer combination comprising, first and second C . glabrata -specific amplification oligomers for amplifying a Candida sp. target nucleic acid region, wherein said target region corresponds to a region of SEQ ID NO: 131 from about nucleotide position 355 to about nucleotide position 554, and wherein the first and second C . glabrata- specific amplification oligomers respectively comprise first and second C . glabrata- specific target-hybridizing sequences; and (2) performing an in vitro nucleic acid amplification reaction, wherein any Candida sp. target nucleic acid, if present in the sample, is used as a template for generating one or more amplification products corresponding to the target region; and (3) detecting the presence or absence of the one or more amplification products, thereby determining the presence or absence of Candida sp. in the sample.

    METHODS AND COMPOSITIONS FOR DETECTING CANDIDA SPECIES

    公开(公告)号:EP4249609A3

    公开(公告)日:2023-12-20

    申请号:EP23169555.2

    申请日:2017-01-04

    IPC分类号: C12Q1/6895

    摘要: There is provided a method for determining the presence or absence of Candida glabrata in a sample, the method comprising: (1) contacting a sample, said sample suspected of containing Candida sp., with a first amplification oligomer combination comprising, first and second C . glabrata -specific amplification oligomers for amplifying a Candida sp. target nucleic acid region, wherein said target region corresponds to a region of SEQ ID NO: 131 from about nucleotide position 355 to about nucleotide position 554, and wherein the first and second C . glabrata- specific amplification oligomers respectively comprise first and second C . glabrata- specific target-hybridizing sequences; and (2) performing an in vitro nucleic acid amplification reaction, wherein any Candida sp. target nucleic acid, if present in the sample, is used as a template for generating one or more amplification products corresponding to the target region; and (3) detecting the presence or absence of the one or more amplification products, thereby determining the presence or absence of Candida sp. in the sample.