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    ESCHERICHIA COLI EXPRESSION VECTOR AND THE USES THEREOF
    1.
    发明公开
    ESCHERICHIA COLI EXPRESSION VECTOR AND THE USES THEREOF 审中-公开
    大肠杆菌表达载体及其应用

    公开(公告)号：EP1925670A4

    公开(公告)日：2009-02-25

    申请号：EP06775269

    申请日：2006-08-02

    申请人： GENEHARBOR HONG KONG TECHNOLOG

    发明人： WANG JUN ,  TSANG WAIKEI ,  YAP HONGKIN ,  LIU ZHAOMING ,  SIU YAULUNG ,  TSANG SUPYIN

    IPC分类号： C12N15/70 ,  C12N9/06 ,  C12N9/14 ,  C12N15/68 ,  C12N15/72 ,  C12P21/02 ,  C12P35/06

    CPC分类号： C12N15/70 ,  C12P21/02

    摘要： The present invention discloses Escherichia coli expression vector, as well as a method for steadily, high-efficiently expressing a required target protein in Escherichia coli using the expression vector. The expression vector is constructed from vector pRSET-A, wherein ampicillin resistance gene in said vector pRSET-A is replaced by kanamycin resistance gene. The expression vector can be used to high-efficiently express enzyme required for the production of 7-aminocephalosporanic acid, meanwhile it does not produce ß-lactamase by which the productivity of 7-aminocephalosporanic acid is reduced. The present invention also discloses two expression vectors, i.e., one expression vector pHS-GHA whose gene product is D-amino acid oxidase mutant GHA, and another expression vector pT7-kan-ACY whose gene product is glutaryl-7-aminocephalosporanic acid acylase of Pseudomonas sp. SE83.