公开(公告)号：EP2106452A2

公开(公告)日：2009-10-07

申请号：EP08705788.1

申请日：2008-01-09

申请人： GENERAL ELECTRIC COMPANY

发明人： NELSON, John R. ,  DUTHIE, Robert Scott ,  FULLER, Carl Williams ,  GROSSMANN, Gregory A. ,  SEKHER, Anuradha

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6858 ,  C12Q1/6844 ,  C12Q2527/101 ,  C12Q2525/101 ,  C12Q2521/301

摘要： Provided herein are nucleic acid synthesis methods and agents that employ an endonuclease for example, endonuclease V, to introduce a nick into a target DNA including one or more inosine, and uses a DNA polymerase to generate amplicons of the target DNA.

摘要翻译： 本文提供了核酸合成方法和试剂，其使用内切核酸酶例如内切核酸酶V将切口引入包含一个或多个肌苷的靶DNA中，并使用DNA聚合酶来产生靶DNA的扩增子。

公开(公告)号：EP2122351A2

公开(公告)日：2009-11-25

申请号：EP08780435.7

申请日：2008-02-20

申请人： General Electric Company

发明人： LU, Su ,  GUI, John Yupeng ,  MOASSER, Bahram ,  CAI, Wei ,  ZHENG, Zhixin ,  NELSON, John R.

IPC分类号： G01N33/532 ,  G01N33/533 ,  G01N33/58

CPC分类号： C12Q1/6834 ,  G01N33/533 ,  G01N33/582

摘要： A composition includes a first probe capable of binding to a first analyte and a first initiator bonded to the first probe. The composition further includes a second probe capable of binding to a second analyte and a second initiator bonded to the second probe. At least one of the first initiator or the second initiator is capable of initiating a controlled polymerization reaction. An associated kit, device, and method are provided.

公开(公告)号：EP2100142A1

公开(公告)日：2009-09-16

申请号：EP07865144.5

申请日：2007-12-04

申请人： GENERAL ELECTRIC COMPANY

发明人： NELSON, John R. ,  MOASSER, Bahram ,  SOOD, Anup ,  GROSSMANN, Gregory A. ,  LV, Su ,  PAN, Zhida ,  QIANG, Liangliang ,  GUI, John Yupeng ,  CAI, Wei ,  ZHENG, Zhixin

IPC分类号： G01N33/532 ,  G01N33/533 ,  G01N33/58 ,  G01N33/542

CPC分类号： G01N33/5308

摘要： A composition includes a first probe, a first initiator component bonded to the first probe, a second probe, and a second initiator component bonded to the second probe. The first probe and the second probe are capable of binding to a single analyte, and the first initiator component and the second initiator component are capable of forming an initiator when present in proximity to each other and when the first probe and the second probe are bonded to the analyte. An associated kit, device, and method are provided.

公开(公告)号：EP2100142B1

公开(公告)日：2012-02-08

申请号：EP07865144.5

申请日：2007-12-04

申请人： General Electric Company

发明人： NELSON, John R. ,  MOASSER, Bahram ,  SOOD, Anup ,  GROSSMANN, Gregory A. ,  LV, Su ,  PAN, Zhida ,  QIANG, Liangliang ,  GUI, John Yupeng ,  CAI, Wei ,  ZHENG, Zhixin

IPC分类号： G01N33/532 ,  G01N33/533 ,  G01N33/58 ,  G01N33/542

CPC分类号： G01N33/5308

摘要： A composition includes a first probe, a first initiator component bonded to the first probe, a second probe, and a second initiator component bonded to the second probe. The first probe and the second probe are capable of binding to a single analyte, and the first initiator component and the second initiator component are capable of forming an initiator when present in proximity to each other and when the first probe and the second probe are bonded to the analyte. An associated kit, device, and method are provided.

公开(公告)号：EP2122351B1

公开(公告)日：2011-10-19

申请号：EP08780435.7

申请日：2008-02-20

申请人： General Electric Company

发明人： LU, Su ,  GUI, John Yupeng ,  MOASSER, Bahram ,  CAI, Wei ,  ZHENG, Zhixin ,  NELSON, John R.

IPC分类号： G01N33/532 ,  G01N33/533 ,  G01N33/58

CPC分类号： C12Q1/6834 ,  G01N33/533 ,  G01N33/582

摘要： A composition includes a first probe capable of binding to a first analyte and a first initiator bonded to the first probe. The composition further includes a second probe capable of binding to a second analyte and a second initiator bonded to the second probe. At least one of the first initiator or the second initiator is capable of initiating a controlled polymerization reaction. An associated kit, device, and method are provided.

公开(公告)号：EP2304044A1

公开(公告)日：2011-04-06

申请号：EP09795031.5

申请日：2009-07-07

申请人： General Electric Company

发明人： NELSON, John R. ,  GAO, Wei

IPC分类号： C12P19/34

CPC分类号： C12N9/1252 ,  C12N9/93

摘要： Methods and kits for DNA repair are provided. The methods and kits described herein repair multiple types of DNA damage. The kit may include a plurality of enzymes to repair a greater variety of lesions than any single enzyme is capable of repairing. Repair of damaged DNA may include releasing damaged bases from the DNA strand, nicking the DNA at the damaged sites, translating the nicks via 5´-3´ exonuclease activity, and sealing the nicks. The enzymes employed in the repair process may then be heat-inactivated, thereby obviating a purification process. The repaired DNA may then be analyzed using a variety of DNA analysis methods.