公开(公告)号：EP3795681B1

公开(公告)日：2024-09-18

申请号：EP20186874.2

申请日：2015-12-16

IPC: C12N9/12 ,  C12Q1/68 ,  C12Q1/6869 ,  C12Q1/686

CPC classification number: C12Q1/6869 ,  C12Q2521/10120130101 ,  C12N9/1252 ,  C12Q1/686 ,  C12Y207/07007

公开(公告)号：EP3619321B1

公开(公告)日：2024-07-17

申请号：EP18793821.2

申请日：2018-05-03

IPC: C12Q1/68 ,  C12N9/12

CPC classification number: C12N9/1252

公开(公告)号：EP4384606A1

公开(公告)日：2024-06-19

申请号：EP22732968.7

申请日：2022-06-02

Applicant: Illumina, Inc.

Inventor： GOLYNSKIY, Misha ,  MATSUURA, Mariko ,  NIRANTAR, Saurabh ,  TABATABAEI GHOMI, Hamed ,  MCDONALD, Seth ,  CRAIG, Ryan ,  PEISAJOVICH, Sergio ,  JOHNSON, Kyrie ,  KLAUSING, Kay ,  MURTFELDT, Eric ,  EXNER, Alexandra

IPC: C12N9/12

CPC classification number: C12N9/1252

公开(公告)号：EP4363564A1

公开(公告)日：2024-05-08

申请号：EP21948680.0

申请日：2021-06-29

Applicant: Agilent Technologies, Inc.

Inventor： CAYOUETTE, Michelle ,  FOX, Jeffrey ,  HANSEN, Connie ,  HOGREFE, Holly ,  WU, Weidong

IPC: C12N9/12 ,  C12Q1/6869

CPC classification number: C12Q1/6869 ,  C12N9/1252 ,  C12Y207/07007 ,  C12N15/52 ,  C12Q1/6844

公开(公告)号：EP3380607A2

公开(公告)日：2018-10-03

申请号：EP16801247.4

申请日：2016-11-25

Applicant: F. Hoffmann-La Roche AG ,  Roche Diagnostics GmbH

Inventor： FRANKLIN, Helen ,  CECH, Cynthia ,  CRAIG, Timothy Kellogg ,  AYER, Aruna ,  DHIMAN, Kirti ,  CHECHELSKI JOHNSTON, Natalie B. ,  MABRY, Joshua N. ,  BIBILLO, Arkadiusz ,  CRISALLI, Peter ,  DAVIS, Randall W.

IPC: C12N9/12 ,  C12Q1/68

CPC classification number: C12N9/1252 ,  B01L9/527 ,  C12N9/96 ,  C12Q1/6806 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q2525/301 ,  C12Q2565/50 ,  C12Q2565/631 ,  C12Y207/07007 ,  C12Q2521/101 ,  C12Q2565/607 ,  C12Q2525/191

Abstract: Disclosed are methods for isolating polymerase complexes from a mixture of polymerase complex components. The polymerase complexes can comprise a nanopore to provide isolated nanopore sequencing complexes. The methods relate to the positive and negative isolation of the polymerase complexes and/or nanopore sequencing complexes. Also disclosed is a nucleic acid adaptor for isolating active polymerase complexes, polymerase complexes comprising the nucleic acid adaptor, and methods for isolating active polymerase complexes using the nucleic acid adaptor.

公开(公告)号：EP3366769A1

公开(公告)日：2018-08-29

申请号：EP18166213.1

申请日：2013-10-16

Applicant: DNA Polymerase Technology, Inc.

Inventor： Kermekchiev, Milko, B. ,  Zhang, Zhian

IPC: C12N9/12 ,  C12Q1/686

CPC classification number: C12Q1/686 ,  C12N9/1252 ,  C12Q2521/101

Abstract: Provided herein are mutant polymerase enzymes resistant to inhibitors encountered in Polymerase Chain Reactions (PCR). Also provided are nucleic acids or consrtucts encoding isolated polypeptides having polymerase activity. Also provided are kits useful for PCR containing isolated polypeptides having polymerase activity or isolated nucleic acids encoding such.

公开(公告)号：EP3208336A4

公开(公告)日：2018-06-20

申请号：EP14904072

申请日：2014-10-14

Applicant: MGI TECH CO LTD

Inventor： JIANG YUAN ,  GENG CHUNYU ,  ZHAO XIA ,  FU SHUJIN ,  HE LINGYU ,  LI YAQIAO ,  SU XIAOSHAN ,  WU FANZI ,  ZHANG WENWEI ,  JIANG HUI ,  ALEXEEV ANDREI ,  DRMANAC RADOJE

IPC: C12N15/11 ,  C12Q1/68 ,  C12Q1/6806 ,  C12Q1/6855 ,  C40B40/06 ,  C40B50/06

CPC classification number: C12N15/1093 ,  C12N9/1205 ,  C12N9/1252 ,  C12N9/16 ,  C12N15/11 ,  C12Q1/68 ,  C12Q1/6806 ,  C12Q1/6855 ,  C12Y207/01078 ,  C12Y207/07007 ,  C12Y301/03001 ,  C40B40/06 ,  C40B50/06 ,  C12Q2521/531 ,  C12Q2525/155 ,  C12Q2525/186 ,  C12Q2525/191 ,  C12Q2525/307 ,  C12Q2535/122 ,  C12Q2537/162 ,  C12Q2563/131

Abstract: Provided is a linker element and a method of using the linker element to construct a sequencing library, wherein the linker element consists of a linker A and a linker B, the linker A is obtained through the complementary pairing of a long nucleic acid strand and a short nucleic acid strand, the 5' end of the long strand has a phosphoric acid modification, and the 3' end of the short strand has an enclosed modification, with enzyme sites in the short strand; and the linker B is a nucleic acid single strand, and the 3' end thereof can be in a complementary pairing with the 5' end of the long strand of the linker A. Using the linker element of the present invention for constructing a sequencing library ensures the linking directionality of the linkers while solving the problems of fragment interlinking, linker self-linking and low linking efficiency, and reducing the purification reaction between steps, shortening the linking time and reducing costs.

公开(公告)号：EP3026111B1

公开(公告)日：2018-06-06

申请号：EP15185052.6

申请日：2012-08-02

Applicant: Thermo Fisher Scientific Baltics UAB

Inventor： SKIRGAILA, Remigijus ,  TUBELEVICIUTE, Agne ,  RIMSELIENE, Renata ,  BURINSKAS, Sigitas

IPC: C12N9/12

CPC classification number: C12N9/1252 ,  C07K2319/21 ,  C12Y207/07007

Abstract: A DNA polymerase mutant comprising a Taq DNA polymerase amino acid sequence with a mutation at one or more of the following selected amino acid positions: E189K, E230K, E507K, H28R, L30R, G38R, F73V, H75R, E76A, E76G, E76K, E90K, K206R, E315K, A348V, L351F, A439T, D452N, G504S, E507A, D551N, L552R, 1553V, D578N, H676R, Q680R, D732G, E734G, E734K, F749V; wherein the polymerase mutant exhibits relative to wild-type DNA polymerase increased polymerase speed, increased affinity to DNA substrate and/or increased resistance to a DNA polymerase inhibitor; and wherein, when the mutation is E507K in combination with two or more further mutations or the mutation is Q680R in combination with four or more further mutations, at least one of the further mutations is at one of the selected amino acid positions; and when the mutation is 1553V, this is not in combination with D551S.

公开(公告)号：EP2971070B1

公开(公告)日：2018-04-25

申请号：EP13712657.9

申请日：2013-03-14

Applicant: Illumina, Inc.

Inventor： CHEN, Cheng-Yao ,  HE, Molly ,  BOMATI, Erin ,  PEISAJOVICH, Sergio

IPC: C12Q1/68 ,  C12N9/12 ,  C12N15/10

CPC classification number: C12Q1/6869 ,  C12N9/1252 ,  C12Q1/6844 ,  C12Y207/07007 ,  C12Q2521/101

Abstract: Presented herein are polymerase enzymes for improved incorporation of nucleotide analogues, in particular nucleotides which are modified at the 3′ sugar hydroxyl, as well as methods and kits using the same.

公开(公告)号：EP3268050A1

公开(公告)日：2018-01-17

申请号：EP16762353.7

申请日：2016-03-08

Applicant: The Trustees of Columbia University in the City of New York

Inventor： LI, Zengmin ,  KALACHIKOV, Sergey ,  JU, Jingyue ,  FULLER, Carl W.

IPC: A61K47/48 ,  C07D237/26 ,  C07D403/04

CPC classification number: C12Q1/6874 ,  A61K47/545 ,  A61K47/62 ,  A61K47/6889 ,  A61K47/69 ,  C07K14/31 ,  C07K19/00 ,  C12N9/1252 ,  C12N9/96 ,  C12Y207/07007

Abstract: The present disclosure relates to compositions and methods based on a fast, efficient chemical reaction for conjugating a pore-forming protein, such as α-hemolysin, to a biomolecule, such as antibodies, receptors, and enzymes, such as DNA polymerase, and the use of such pore-forming protein conjugates in nanopore devices and methods.