公开(公告)号：EP0340783B1

公开(公告)日：1993-10-20

申请号：EP89108110.1

申请日：1989-05-05

申请人： HITACHI, LTD.

发明人： Matsuzaki, Harumi ,  Haga, Ryoichi ,  Nishimura, Yuusaku ,  Baba, Kenji ,  Maruhashi, Fumio ,  Nishimura, Nobuko ,  Ishida, Masahiko ,  Saitoh, Setuo

IPC分类号： C12M3/00 ,  C12M1/34 ,  C12M1/36

CPC分类号： G01N33/5005 ,  C12M41/36 ,  C12M41/46 ,  C12N1/12 ,  C12N1/20 ,  Y10S435/808 ,  Y10S435/813

公开(公告)号：EP0468705A3

公开(公告)日：1992-04-15

申请号：EP91306551.2

申请日：1991-07-18

申请人： HITACHI, LTD.

发明人： Maruhashi, Fumio ,  Haga, Ryoichi ,  Nakano, Ryusei ,  Nishimura, Nobuko ,  Matsuzaki, Harumi

IPC分类号： G01N15/14 ,  C12M1/34 ,  F23N5/08

CPC分类号： C12M41/46 ,  C12M41/36 ,  G01N15/1468 ,  Y10S435/808

摘要： In order to investigate an object, such as a culture of micro-organisms, the image of the object is repeatedly picked up at two different magnifications by a suitable image pick-up device (21, 22, 24). The images at the two different magnifications are then analysed by a suitable picture image recognition device (25, 31) and the results of the repeated analysis are compared, thereby to derive a measurement of the change in the object with time. Thus, for a culture of micro-organisms, the number of cells can be determined at one magnification and the number of microscopic spherical bodies can be determined at another magnification, to obtain a measure of the activity of the culture. Preferably, one or more conditions of the object are then controlled on the basis of the measurement of change.

公开(公告)号：EP0708390A2

公开(公告)日：1996-04-24

申请号：EP95118768.1

申请日：1990-03-13

申请人： HITACHI, LTD.

发明人： Baba, Kenji ,  Enbutsu, Ichiro ,  Watanabe, Shoji ,  Yahagi, Hayao ,  Maruhashi, Fumio ,  Matsuzaki, Harumi ,  Matsumoto, Hiroshi ,  Nogita, Shunsuke ,  Yoda, Mikio ,  Hara, Naoki

IPC分类号： G05B13/02

CPC分类号： G05B13/027 ,  C02F1/008 ,  G05B13/0285 ,  Y10S706/903 ,  Y10S706/906

摘要： A method for extracting as knowledge causal relationships between input variables and an output variable of a neural circuit model, said neural circuit model being of a hierarchical structure constructed of an input layer, at least one hidden layer and an output layer and having performed learning a limited number of times by determining weight factors between mutually-connected neuron element models in different layers of the input layer, hidden layer and output layer, wherein with respect to plural routes extending from a neuron element model, corresponding to a particular input variable, of the input layer to a neuron element model, corresponding to a particular output variable, of the output layer by way of the individual neuron element models of the hidden layer, a product of the weight factors for each of the routes is determined, and the products for the plural routes are summed, whereby the sum is employed as a measure for the determination of the causal relationship between the particular input variable and the particular output variable.

摘要翻译： 一种用于提取输入变量与神经电路模型的输出变量之间的知识因果关系的方法，所述神经电路模型是由输入层，至少一个隐藏层和输出层构成的分层结构，并且已经执行了学习 通过确定输入层，隐层和输出层的不同层中相互连接的神经元元素模型之间的权重因子的有限次数，其中相对于从对应于特定输入变量的神经元元素模型延伸的多个路线， 通过隐藏层的各个神经元元素模型将输入层输入到输出层对应于特定输出变量的神经元元素模型，确定每个路线的权重因子的乘积，并且产品 多数路线被归纳为总和，由此作为衡量第th之间因果关系的措施 特定的输入变量和特定的输出变量。

公开(公告)号：EP0340783A2

公开(公告)日：1989-11-08

申请号：EP89108110.1

申请日：1989-05-05

申请人： HITACHI, LTD.

发明人： Matsuzaki, Harumi ,  Haga, Ryoichi ,  Nishimura, Yuusaku ,  Baba, Kenji ,  Maruhashi, Fumio ,  Nishimura, Nobuko ,  Ishida, Masahiko ,  Saitoh, Setuo

IPC分类号： C12M3/00 ,  C12M1/34 ,  C12M1/36

CPC分类号： G01N33/5005 ,  C12M41/36 ,  C12M41/46 ,  C12N1/12 ,  C12N1/20 ,  Y10S435/808 ,  Y10S435/813

摘要： This invention provides an apparatus for culturing animal cells, which comprises diagnosis means to make it possible to diagnose by measuring sizes of the cells by means of image processing while a system of culturing the animal cells is cut off from the outside of the apparatus system, control means to control the culture conditions of a culturing cistern on the basis of a result of the diagnosis, and culture medium re­generating means to remove waste contents in a culture medium. In the apparatus of this invention, the culture system and diagnosis system are cut off from the outside of the apparatus system. Therefore, no infection of miscellaneous microorganism, etc., occurs, nor does consumption of valuable cells and products occur. Further, since it is possible to easily obtain data of the proportions of division potential-possessing cells and living cells, the environmental factors to the culturing cistern can be easily and instantly controlled based on the data, and therefore, the culturing efficiency can be enhanced.

摘要翻译： 本发明提供的装置用于培养动物细胞的方法，其包括诊断手段，使之有可能通过测量细胞的大小，通过图像处理的手段，同时培养动物细胞的系统是从装置系统的外部切断诊断， 控制装置，以控制该诊断结果的基础上，一个水箱培养的培养条件，和培养基再生装置以除去在培养基中废物的内容。 在本发明的装置中，所述培养系统和诊断系统从装置系统的外部切断。因此，没有感染杂微生物等，该情况发生时，也不有价值的细胞和产品发生的消耗。 另外，由于能够容易地获得分割电位波塞的比例的数据唱细胞和活细胞，环境因素对培养水箱能够容易地并即时控制基于所述数据，并且因此，培养效率提高 ，

公开(公告)号：EP0708390A3

公开(公告)日：1997-04-02

申请号：EP95118768.1

申请日：1990-03-13

申请人： HITACHI, LTD.

发明人： Baba, Kenji ,  Enbutsu, Ichiro ,  Watanabe, Shoji ,  Yahagi, Hayao ,  Maruhashi, Fumio ,  Matsuzaki, Harumi ,  Matsumoto, Hiroshi ,  Nogita, Shunsuke ,  Yoda, Mikio ,  Hara, Naoki

IPC分类号： G05B13/02

CPC分类号： G05B13/027 ,  C02F1/008 ,  G05B13/0285 ,  Y10S706/903 ,  Y10S706/906

摘要： A method for extracting as knowledge causal relationships between input variables and an output variable of a neural circuit model, said neural circuit model being of a hierarchical structure constructed of an input layer, at least one hidden layer and an output layer and having performed learning a limited number of times by determining weight factors between mutually-connected neuron element models in different layers of the input layer, hidden layer and output layer, wherein with respect to plural routes extending from a neuron element model, corresponding to a particular input variable, of the input layer to a neuron element model, corresponding to a particular output variable, of the output layer by way of the individual neuron element models of the hidden layer, a product of the weight factors for each of the routes is determined, and the products for the plural routes are summed, whereby the sum is employed as a measure for the determination of the causal relationship between the particular input variable and the particular output variable.

公开(公告)号：EP0468705A2

公开(公告)日：1992-01-29

申请号：EP91306551.2

申请日：1991-07-18

申请人： HITACHI, LTD.

发明人： Maruhashi, Fumio ,  Haga, Ryoichi ,  Nakano, Ryusei ,  Nishimura, Nobuko ,  Matsuzaki, Harumi

IPC分类号： G01N15/14 ,  C12M1/34 ,  F23N5/08

CPC分类号： C12M41/46 ,  C12M41/36 ,  G01N15/1468 ,  Y10S435/808

摘要： In order to investigate an object, such as a culture of micro-organisms, the image of the object is repeatedly picked up at two different magnifications by a suitable image pick-up device (21, 22, 24). The images at the two different magnifications are then analysed by a suitable picture image recognition device (25, 31) and the results of the repeated analysis are compared, thereby to derive a measurement of the change in the object with time. Thus, for a culture of micro-organisms, the number of cells can be determined at one magnification and the number of microscopic spherical bodies can be determined at another magnification, to obtain a measure of the activity of the culture. Preferably, one or more conditions of the object are then controlled on the basis of the measurement of change.

摘要翻译： 为了研究诸如微生物的培养物的对象，通过合适的图像拾取装置（21,22,24）以两种不同的放大率重复地拾取物体的图像。 然后通过适当的图像图像识别装置（25,31）分析两个不同放大倍率的图像，并比较重复分析的结果，由此导出对象随时间变化的测量。 因此，对于微生物的培养物，可以在一个放大倍数下测定细胞数，并且可以在另一放大倍数下确定微球体的数量，以获得培养物的活性的量度。 优选地，基于变化的测量来控制对象的一个​​或多个条件。

公开(公告)号：EP0340783A3

公开(公告)日：1990-07-18

申请号：EP89108110.1

申请日：1989-05-05

申请人： HITACHI, LTD.

发明人： Matsuzaki, Harumi ,  Haga, Ryoichi ,  Nishimura, Yuusaku ,  Baba, Kenji ,  Maruhashi, Fumio ,  Nishimura, Nobuko ,  Ishida, Masahiko ,  Saitoh, Setuo

IPC分类号： C12M3/00 ,  C12M1/34 ,  C12M1/36

CPC分类号： G01N33/5005 ,  C12M41/36 ,  C12M41/46 ,  C12N1/12 ,  C12N1/20 ,  Y10S435/808 ,  Y10S435/813

摘要： This invention provides an apparatus for culturing animal cells, which comprises diagnosis means to make it possible to diagnose by measuring sizes of the cells by means of image processing while a system of culturing the animal cells is cut off from the outside of the apparatus system, control means to control the culture conditions of a culturing cistern on the basis of a result of the diagnosis, and culture medium re­generating means to remove waste contents in a culture medium. In the apparatus of this invention, the culture system and diagnosis system are cut off from the outside of the apparatus system. Therefore, no infection of miscellaneous microorganism, etc., occurs, nor does consumption of valuable cells and products occur. Further, since it is possible to easily obtain data of the proportions of division potential-possessing cells and living cells, the environmental factors to the culturing cistern can be easily and instantly controlled based on the data, and therefore, the culturing efficiency can be enhanced.