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1.发明授权Apparatus for culturing animal cells, method of culturing thereof and diagnostics of the culture 失效对于动物细胞，培养方法以及用于检测培养装置的培养装置。
公开(公告)号：EP0340783B1
公开(公告)日：1993-10-20
申请号：EP89108110.1
申请日：1989-05-05
申请人： HITACHI, LTD.
发明人： Matsuzaki, Harumi , Haga, Ryoichi , Nishimura, Yuusaku , Baba, Kenji , Maruhashi, Fumio , Nishimura, Nobuko , Ishida, Masahiko , Saitoh, Setuo
CPC分类号： G01N33/5005 , C12M41/36 , C12M41/46 , C12N1/12 , C12N1/20 , Y10S435/808 , Y10S435/813
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公开(公告)号：EP0371783A3
公开(公告)日：1991-06-12
申请号：EP89312421.4
申请日：1989-11-29
申请人： HITACHI, LTD.
IPC分类号： C12M3/00
摘要： A culturing method and system are disclosed in which a cell culture broth is withdrawn from a culturing apparatus (1) to a filtration apparatus (6). The withdrawn broth is brought into contact with a membrane (7) for precision filtration. Then a non-toxic gas is blown into the broth by a sparger (9) while filtering the broth to separate the cells from waste liquid. Foams occurring on the surface of the broth due to gas bubbling are broken up by special layers (8). The separated cells are returned to the culture apparatus and the filtrate is withdrawn from the filtration apparatus to a filtrate tank (13). The method and system have such advantages that a perfusion culture of a long period of time can be realized because the clogging of the filtration membrane is effectively prevented.
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3.发明公开Thermostable glucoamylase, a method for production of glucose using same and a plant for production thereof 失效为通过使用相同的装置和它们的制造生产葡萄糖的热稳定性的葡糖淀粉酶处理。
公开(公告)号：EP0255124A2
公开(公告)日：1988-02-03
申请号：EP87111005.2
申请日：1987-07-29
申请人： HITACHI, LTD.
CPC分类号： C12N9/2428 , C12P19/20 , C12R1/145
摘要： The thermostable glucoamylase of the present invention is highly active even in acidic pH ranges at high temperatures of 70°C or above.
According to the process for production of glucose and production plant of the present invention, the liquefaction and saccharification of starch can be performed, without requiring either any cooling step or any neutralizing step with alkalis, because of the use of the thermostable glucoamylase of the present invention in combination with known acid-resistant and thermostable α-amylase.摘要翻译： 本发明的热塑性表葡糖淀粉酶是具有高活性，即使在在70℃或以上的高温酸性pH范围。 。根据生产葡萄糖和本发明中，液化和糖化淀粉的生产设备的过程可以进行，而不需要要么任何冷却步骤或与碱的任何中和步骤中，因为使用了本的热表葡糖淀粉酶的 发明中与已知的耐酸和热稳定α淀粉酶表组合。
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4.发明公开Apparatus for culturing animal cells, method of culturing thereof and diagnostics of the culture 失效用于培养动物细胞的装置，其培养方法和文化诊断
公开(公告)号：EP0340783A3
公开(公告)日：1990-07-18
申请号：EP89108110.1
申请日：1989-05-05
申请人： HITACHI, LTD.
发明人： Matsuzaki, Harumi , Haga, Ryoichi , Nishimura, Yuusaku , Baba, Kenji , Maruhashi, Fumio , Nishimura, Nobuko , Ishida, Masahiko , Saitoh, Setuo
CPC分类号： G01N33/5005 , C12M41/36 , C12M41/46 , C12N1/12 , C12N1/20 , Y10S435/808 , Y10S435/813
摘要： This invention provides an apparatus for culturing animal cells, which comprises diagnosis means to make it possible to diagnose by measuring sizes of the cells by means of image processing while a system of culturing the animal cells is cut off from the outside of the apparatus system, control means to control the culture conditions of a culturing cistern on the basis of a result of the diagnosis, and culture medium regenerating means to remove waste contents in a culture medium. In the apparatus of this invention, the culture system and diagnosis system are cut off from the outside of the apparatus system. Therefore, no infection of miscellaneous microorganism, etc., occurs, nor does consumption of valuable cells and products occur. Further, since it is possible to easily obtain data of the proportions of division potential-possessing cells and living cells, the environmental factors to the culturing cistern can be easily and instantly controlled based on the data, and therefore, the culturing efficiency can be enhanced.
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5.发明公开
公开(公告)号：EP0263484A3
公开(公告)日：1990-06-13
申请号：EP87114567.8
申请日：1987-10-06
申请人： HITACHI, LTD.
CPC分类号： C12N9/2428 , C07H3/06 , C08B35/00 , C12N9/2414 , C12N9/2425 , C12P19/14
摘要： Cross-linked polyglucans obtained by cross-linking polyglucans having branched chains intermolecularly and/or intramolecularly or cross-linked homooligomers obtained by cross-linking α-1,4-linked homooligomers of glucose intermolecularly can selectively adsorb glucoamylase and/or β-amylase thereto. By the use of such three-dimensional, cross-linked high molecular weight substances, glucoamylase and β-amylase can be isolated and purified extremely efficiently.
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公开(公告)号：EP0368621A2
公开(公告)日：1990-05-16
申请号：EP89311517.0
申请日：1989-11-07
申请人： HITACHI, LTD.
CPC分类号： B04B5/00 , B04B15/12 , C12M47/02 , C12N1/02 , Y10T436/25375
摘要： A centrifugal separation method for living cells is disclosed in which a cell containing solution is charged in a rotor chamber confined by a steam pressure-resistant wall. A rotor having septa is disposed in the rotor chamber. The septa are rotated in the same direction at the same angular velocity as the rotor while at least part thereof is immersed in a solution in the rotor when cells are to be centrifuged from the cell-containing solution. After the cells are separated and adhered to the inner surface of the rotor, a supernatant in the rotor is discharged from the rotor. Then a fresh cultivating medium is introduced into the rotor, and the septa and the rotor are rotated at mutually different angular velocities while at least part of the septa is immersed in the fresh medium in the rotor. As a result, the cells are again suspended in the fresh medium.
摘要翻译： 公开了一种用于活细胞的离心分离方法，其中将含细胞的溶液装入由耐蒸汽压壁限制的转子室中。 具有隔片的转子设置在转子室中。 隔片以与转子相同的角速度沿相同的方向旋转，而当细胞从含细胞溶液离心时，其至少一部分浸入到转子中的溶液中。 在细胞分离并粘附到转子的内表面之后，转子中的上清液从转子中排出。 然后将新鲜的栽培培养基引入转子中，并且隔片和转子以相互不同的角速度旋转，同时至少部分隔片浸入转子中的新鲜培养基中。 结果，细胞再次悬浮在新鲜培养基中。
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公开(公告)号：EP0180952A2
公开(公告)日：1986-05-14
申请号：EP85114004.6
申请日：1985-11-04
申请人： HITACHI, LTD.
CPC分类号： C12N9/2417 , C12P19/14 , Y10S435/842
摘要： This invention relates to a process for liquefying starch. More particularly it relates to an appropriate process for liquefying starch by using a thermostable a-amylase which has an optimum working pH range in an acidic region and a low calcium requirement.
This invention provides a process for liquefying starch by using a calcium-requiring, thermostable a-amylase which requires a calcium concentration of 10 µM or below under a thermostable condition.
In the process of the present invention, it is unnecessary to add a calcium salt in liquefying starch and the starch slurry can be reacted while remaining acidic without neutralization, so that the load at the desalting step of the product can be significantly relieved.-
公开(公告)号：EP0155577A1
公开(公告)日：1985-09-25
申请号：EP85102336.6
申请日：1985-03-01
申请人： HITACHI, LTD.
摘要： 57 This invention provides a process for easily recovering nearly white starch and protein having a high commercial value by depressing darkening of crushed slurry and juice in producing starch and protein of a subterranean stem.
The above-mentioned process is characterized by adding at least one member selected from the group consisting of sodium thiosulfate, potassium thiosulfate, sodium hydrogen sulfite, sodium sulfite and potassium sulfite at the time of crushing the subterranean stem.-
公开(公告)号：EP0371783B1
公开(公告)日：1994-04-06
申请号：EP89312421.4
申请日：1989-11-29
申请人： HITACHI, LTD.
IPC分类号： C12M3/00
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10.发明公开Thermostable glucoamylase, a method for production of glucose using same and a plant for production thereof 失效可热稳定的葡糖淀粉酶，使用其生产葡萄糖的方法和用于生产的植物
公开(公告)号：EP0255124A3
公开(公告)日：1989-04-12
申请号：EP87111005.2
申请日：1987-07-29
申请人： HITACHI, LTD.
CPC分类号： C12N9/2428 , C12P19/20 , C12R1/145
摘要： The thermostable glucoamylase of the present invention is highly active even in acidic pH ranges at high temperatures of 70°C or above. According to the process for production of glucose and production plant of the present invention, the liquefaction and saccharification of starch can be performed, without requiring either any cooling step or any neutralizing step with alkalis, because of the use of the thermostable glucoamylase of the present invention in combination with known acid-resistant and thermostable α-amylase.
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