公开(公告)号：EP1947453A1

公开(公告)日：2008-07-23

申请号：EP06822174.6

申请日：2006-10-18

申请人： National Institute of Advanced Industrial Science and Technology ,  Sharp Kabushiki Kaisha ,  Toppan Printing Co., Ltd. ,  Katayanagi Institute

发明人： SUZUKI, Yoshio ,  YOKOYAMA, Kenji ,  NAMATAME, Ichiji

IPC分类号： G01N27/447

CPC分类号： G01N27/44726

摘要： A method for analyzing proteins with the use of electrophoresis is provided, which makes it possible to rapidly and conveniently analyze a great variety of proteins with high sensitivity. The method for analyzing a protein in a sample comprises setting a sample in a carrier for electrophoresis, performing electrophoresis for the sample using a buffer for electrophoresis in which a labeling compound represented by formula I:

wherein R 1 is an aryl group or a heteroaryl group that may be substituted, R 2 is a heterocyclic group that may be substituted, and n is an integer between 1 and 5, is dissolved, and then detecting a complex of the compound of formula I and the protein via spectrophotometrical measurement.

摘要翻译： 提供了利用电泳分析蛋白质的方法，可以快速方便地分析高灵敏度的多种蛋白质。 用于分析样品中的蛋白质的方法包括将载体中的样品设定为电泳，使用电泳缓冲液进行电泳，其中由式I表示的标记化合物：其中R 1为芳基或杂芳基 可以被取代，R 2是可以被取代的杂环基，并且n是1和5之间的整数，然后通过分光光度测量法检测式I化合物与蛋白质的络合物。

公开(公告)号：EP1947453B1

公开(公告)日：2013-10-09

申请号：EP06822174.6

申请日：2006-10-18

申请人： National Institute of Advanced Industrial Science and Technology ,  Sharp Kabushiki Kaisha ,  Toppan Printing Co., Ltd. ,  Katayanagi Institute

发明人： SUZUKI, Yoshio ,  YOKOYAMA, Kenji ,  NAMATAME, Ichiji

IPC分类号： G01N27/447

CPC分类号： G01N27/44726

公开(公告)号：EP1548429A1

公开(公告)日：2005-06-29

申请号：EP03799096.7

申请日：2003-09-05

申请人： Katayanagi Institute ,  National Institute of Advanced Industrial Science and Technology

发明人： HIRATSUKA, Atsunori ,  YANO, Kazuyoshi ,  KARUBE, Isao ,  TSAI, Shuo-Wen ,  YOKOYAMA, Kenji ,  KOIDE, Satoshi ,  AKIMOTO, Takuo

IPC分类号： G01N27/447

CPC分类号： B82Y30/00 ,  G01N27/447 ,  G01N27/44752

摘要： An objective of the present invention is to provide electrophoretic separation methods and devices that enable the various features of a substrate surface that comes in contact with an electrophoresis medium to be controlled.
The present invention provides methods for electrophoresing substances, which comprises the steps of:

(a) adding a substance to be analyzed to an electrophoresis medium retained in a substrate, whose surface that has come in contact with the electrophoresis medium has been coated with a polymer membrane; and
(b) adding electrophoretic pressure to the electrophoresis medium.

For example, the use of a plasma-polymerized membrane allows the formation of a membrane with homogeneous quality and thickness on the surface of an arbitrary shape. In addition, desired characteristics can be conferred on the surface through selection of monomeric substances. Protein adsorption onto micro-chips can be effectively prevented as well.

摘要翻译： 本发明的目的是提供一种电泳分离方法和装置，其能够使与基质表面接触的电泳介质的各种特征得以控制。 本发明提供了电泳物质的方法，其包括以下步骤：（a）将待分析物质加入保留在与电泳介质接触的表面的基质中的电泳介质中 涂有聚合物膜; 和（b）向电泳介质中加入电泳压力。 例如，使用等离子体聚合膜允许在任意形状的表面上形成均匀质量和厚度的膜。 此外，可以通过选择单体物质在表面上赋予所需的特性。 可以有效地防止蛋白质在微芯片上的吸附。