公开(公告)号：EP1947453A1

公开(公告)日：2008-07-23

申请号：EP06822174.6

申请日：2006-10-18

申请人： National Institute of Advanced Industrial Science and Technology ,  Sharp Kabushiki Kaisha ,  Toppan Printing Co., Ltd. ,  Katayanagi Institute

发明人： SUZUKI, Yoshio ,  YOKOYAMA, Kenji ,  NAMATAME, Ichiji

IPC分类号： G01N27/447

CPC分类号： G01N27/44726

摘要： A method for analyzing proteins with the use of electrophoresis is provided, which makes it possible to rapidly and conveniently analyze a great variety of proteins with high sensitivity. The method for analyzing a protein in a sample comprises setting a sample in a carrier for electrophoresis, performing electrophoresis for the sample using a buffer for electrophoresis in which a labeling compound represented by formula I:

wherein R 1 is an aryl group or a heteroaryl group that may be substituted, R 2 is a heterocyclic group that may be substituted, and n is an integer between 1 and 5, is dissolved, and then detecting a complex of the compound of formula I and the protein via spectrophotometrical measurement.

摘要翻译： 提供了利用电泳分析蛋白质的方法，可以快速方便地分析高灵敏度的多种蛋白质。 用于分析样品中的蛋白质的方法包括将载体中的样品设定为电泳，使用电泳缓冲液进行电泳，其中由式I表示的标记化合物：其中R 1为芳基或杂芳基 可以被取代，R 2是可以被取代的杂环基，并且n是1和5之间的整数，然后通过分光光度测量法检测式I化合物与蛋白质的络合物。

公开(公告)号：EP1947453B1

公开(公告)日：2013-10-09

申请号：EP06822174.6

申请日：2006-10-18

申请人： National Institute of Advanced Industrial Science and Technology ,  Sharp Kabushiki Kaisha ,  Toppan Printing Co., Ltd. ,  Katayanagi Institute

发明人： SUZUKI, Yoshio ,  YOKOYAMA, Kenji ,  NAMATAME, Ichiji

IPC分类号： G01N27/447

CPC分类号： G01N27/44726