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    THERMOSTABLE L-RIBOSE ISOMERASE, PROCESS FOR PRODUCING THE SAME AND USE THEREOF
    3.
    发明公开
    THERMOSTABLE L-RIBOSE ISOMERASE, PROCESS FOR PRODUCING THE SAME AND USE THEREOF 有权
    热稳定剂L-RIBOSE-ISOMERASE，VERFAHREN ZU DEREN HERSTELLUNG UND ANWENDUNG DAVON

    公开(公告)号：EP2103625A1

    公开(公告)日：2009-09-23

    申请号：EP07739605.9

    申请日：2007-03-26

    申请人： National University Corporation Kagawa University ,  Rare Sugar Production Technical Research Laboratories, LLC ,  Fushimi Pharmaceutical Company, Limited ,  Kabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo ,  Matsutani Chemical Industry Co., Ltd.

    发明人： IZUMORI, Ken ,  MORIMOTO, Kenji ,  TAKATA, Goro ,  TOKUDA, Masaaki ,  TSUJISAKA, Yoshio ,  TAKESHITA, Kei ,  TSUSAKI, Keiji ,  OKUMA, Kazuhiro

    IPC分类号： C07K14/195 ,  C12N1/20 ,  C12N9/90 ,  C12P19/24

    CPC分类号： C12N9/90 ,  C12P19/24 ,  C12R1/01

    摘要： Object: To provide a thermostable L-ribose isomerase.
    Means for Resolution: The thermostable L-ribose isomerase with MW. 32,000 (by SDS-PAGE), optimal temperature of 45°C, optimal pH of pH 9. 0 (glycine-NaOH buffer), and stable physicochemical properties such as temperature stability up to 45°C during thermal treatment at pH 9.0 for 10 minutes, and with an action to isomerize L-ribose to generate L-ribulose or of inversely to isomerize L-ribulose to generate L-ribose. A conversion method between an aldose and a ketose comprising allowing the thermostable L-ribose isomerase as an enzyme derived from (1) Raoultella ornithinolytica strain MB426 (NITE BP-277) to interact with an aldose selected from L-ribose, D-lyxose, D-tallose, D-mannose, L-allose and L-gulose to isomerize the aldose to generate a ketose selected from the individually corresponding L-ribulose, D-xylulose, D-tagatose, D-fructose, L-psicose and L-sorbose or to interact with a ketose selected from L-ribulose, D-xylulose, D-tagatose, D-fructose, L-psicose and L-sorbose to isomerize the ketose to generate an aldose selected from the individually corresponding L-ribose, D-lyxose, D-tallose, D-mannose, L-allose and L-gulose.

    摘要翻译： 目的：提供热稳定的L-核糖异构酶。 解决方法：具有MW的热稳定的L-核糖异构酶。 32,000（通过SDS-PAGE），最适温度为45℃，pH为9.0（甘氨酸 - NaOH缓冲液）的最佳pH，以及稳定的物理化学性质，例如在pH 9.0的热处理期间高达45℃的温度稳定性为10 分钟，并且具有异构化L-核糖以产生L-核酮糖的作用或者与L-核酮糖异构化以产生L-核糖的作用。 醛糖和酮糖之间的转化方法，其包括使热稳定的L-核糖异构酶作为源自（1）鸟氨酸龙胆菌株MB426（NITE BP-277）的酶与选自L-核糖，D-赖索糖， D-山梨糖醇，D-甘露糖，L-丙氨酸和L-古洛糖醇，使醛糖异构化，产生选自单独对应的L-核酮糖，D-木酮糖，D-塔格糖，D-果糖，L-灵芝蛋白和L- 山梨糖或与选自L-核酮糖，D-木酮糖，D-塔格糖，D-果糖，L-灵敏糖和L-山梨糖的酮糖相互作用以使酮糖异构化以产生选自相应的L-核糖，D D-糖精，D-托洛糖，D-甘露糖，L-阿洛糖和L-古洛糖。