公开(公告)号：EP1694274A2

公开(公告)日：2006-08-30

申请号：EP04813334.2

申请日：2004-12-03

申请人： Neose Technologies, Inc.

发明人： DEFREES, Shawn ,  CLAUSEN, Henrik ,  ZOPF, David, A. ,  WANG, Zhi-Guang ,  SCHWARTZ, Marc ,  WU, Bingyuan ,  BOWE, Caryn

IPC分类号： A61K6/00

CPC分类号： A61K38/193 ,  A61K47/60 ,  C07K14/535

摘要： The present invention provides conjugates between Granulocyte Colony Stimulating Factor and PEG moieties. The conjugates are linked via an intact glycosyl linking group that is interposed between and covalently attached to the peptide and the modifying group. The conjugates are formed from both glycosylated and unglycosylated peptides by the action of a glycosyltransferase. The glycosyltransferase ligates a modified sugar moiety onto either an amino acid or glycosyl residue on the peptide. Also provided are pharmaceutical formulations including the conjugates. Methods for preparing the conjugates are also within the scope of the invention.

公开(公告)号：EP1858543A2

公开(公告)日：2007-11-28

申请号：EP06717998.6

申请日：2006-01-10

申请人： Neose Technologies, Inc.

发明人： DEFREES, Shawn ,  CLAUSEN, Henrik ,  ZOPF, David, A. ,  BOWE, Caryn ,  TAUDTE, Susann ,  FELO, Michael ,  WILLETT, Walter, S.

IPC分类号： A61K38/19 ,  C07K14/535

CPC分类号： C07K14/535 ,  A61K38/00 ,  A61K47/549 ,  A61K47/60 ,  A61K47/62 ,  C07K1/1077 ,  C07K1/18

摘要： The present invention provides conjugates between Granulocyte Colony Stimulating Factor and PEG moieties. The conjugates are linked via an intact glycosyl linking group that is interposed between and covalently attached to the peptide and the modifying group. The conjugates are formed from both glycosylated and unglycosylated peptides by the action of a glycosyltransferase. The glycosyltransferase ligates a modified sugar moiety onto either an amino acid or glycosyl residue on the peptide. Also provided are pharmaceutical formulations including the conjugates. Methods for preparing the conjugates are also within the scope of the invention.

公开(公告)号：EP1799249A2

公开(公告)日：2007-06-27

申请号：EP05803800.1

申请日：2005-09-12

申请人： Neose Technologies, Inc.

发明人： DEFREES, Shawn ,  ZOPF, David, A. ,  CLAUSEN, Henrik ,  XING, Ruye

IPC分类号： A61K38/21

CPC分类号： A61K38/212 ,  A61K47/60 ,  Y02A50/463 ,  Y02A50/473

摘要： The present invention provides IFN-α conjugates including IFN-α peptides and modifying groups such as PEG moieties. The IFN-α peptide and modifying group are linked via an intact glycosyl linking group interposed between and covalently attached to the IFN-α peptide and the modifying group. The IFN-α conjugates are formed from glycosylated peptides by the action of a glycosyltransferase. The glycosyltransferase ligates a modified sugar onto an amino acid or a glycosyl residue on the IFN-α peptide. Also provided are methods for preparing the IFN-α conjugates, methods for treating various disease conditions with the IFN-α conjugates, and pharmaceutical formulations including the IFN-α conjugates.

摘要翻译： 本发明提供了包括IFN-α肽和修饰基团如PEG部分的IFN-α缀合物。 IFN-α肽和修饰基团通过置于IFN-α肽和修饰基团之间并共价连接的完整糖基连接基团连接。 IFN-α缀合物由糖基化肽通过糖基转移酶的作用形成。 糖基转移酶将修饰的糖连接到IFN-α肽上的氨基酸或糖基残基上。 还提供了用于制备IFN-α缀合物的方法，用IFN-α缀合物治疗各种疾病的方法，以及包含IFN-α缀合物的药物制剂。

公开(公告)号：EP1771066A2

公开(公告)日：2007-04-11

申请号：EP05791089.5

申请日：2005-07-13

申请人： Neose Technologies, Inc.

发明人： DEFREES, Shawn ,  ZOPF, David, A. ,  WANG, Zhi-Guang ,  CLAUSEN, Henrik

IPC分类号： A01N43/04

CPC分类号： C07K14/605 ,  A61K38/00

摘要： The present invention provides polypeptides that include an O-linked glycoconjugate in which a species such as a water-soluble polymer, a therapeutic agent of a biomolecule is covalently linked through an intact O-linked glycosyl residue to the polypeptide. The polypeptides of the invention include wild-type peptides and mutant peptides that include an O-linked glycosylation site that is not present in the wild-type peptide. Also provided are methods of making the peptides of the invention and methods, pharmaceutical compositions containing the peptides and methods of treating, ameliorating or preventing diseased in mammals by administering an amount of a peptide of the invention sufficient to achieve the desired response.

摘要翻译： 本发明提供了包括O-连接的糖缀合物的多肽，其中诸如水溶性聚合物（生物分子的治疗剂）的物质通过完整的O-连接的糖基残基共价连接至多肽。 本发明的多肽包括野生型肽和包含野生型肽中不存在的O-联糖基化位点的突变肽。 还提供了制备本发明的肽的方法和方法，含有所述肽的药物组合物以及通过施用足以获得所需反应的量的本发明肽来治疗，改善或预防哺乳动物患病的方法。

公开(公告)号：EP1765993A2

公开(公告)日：2007-03-28

申请号：EP05759289.1

申请日：2005-06-03

申请人： Neose Technologies, Inc.

发明人： JOHNSON, Karl F. ,  HAKES, David ,  WEI, Ge ,  LIU, Li ,  SARIBAS, Sami ,  SJOBERG, Eric ,  CLAUSEN, Henrik ,  BENNETT, Eric, Paul ,  MOBASSERI, Aliakbar

IPC分类号： C12N9/10

CPC分类号： C12N9/1081 ,  C07K2319/20 ,  C07K2319/40

摘要： The present invention features compositions and methods related to truncated mutants of ST6GalNAcI. In particular, the invention features truncated human, mouse, and chicken ST6GalNAcI polypeptides. The invention also features nucleic acids encoding such truncated polyeptides, as well as vectors, host cells, expression systems, and methods of expressing and using such polypeptides.

公开(公告)号：EP1765853A2

公开(公告)日：2007-03-28

申请号：EP05711345.8

申请日：2005-01-10

申请人： Neose Technologies, Inc.

发明人： DEFREES, Shawn ,  ZOPF, David, A. ,  WANG, Zhi-Guang ,  CLAUSEN, Henrik

IPC分类号： C07K9/00

CPC分类号： A61K38/193 ,  C07K1/1077 ,  C07K9/00 ,  C07K9/001 ,  C07K14/535

摘要： The present invention provides polypeptides that include an O-linked glycosylation site that is not present in the wild-type peptide. The polypeptides of the invention include glycoconjugates in which a species such as a water-soluble polymer, a therapeutic agent of a biomolecule is covalently linked through an intact O-linked glycosyl residue to the polypeptide. Also provided are methods of making the peptides of the invention and methods, pharmaceutical compositions containing the peptides and methods of treating, ameliorating or preventing diseased in mammals by administering an amount of a peptide of the invention sufficient to achieve the desired response.

公开(公告)号：EP1624847A2

公开(公告)日：2006-02-15

申请号：EP04751591.1

申请日：2004-05-07

申请人： Neose Technologies, Inc.

发明人： CLAUSEN, Henrik ,  DEFREES, Shawn

IPC分类号： A61K6/00

CPC分类号： C07K14/61 ,  A61K38/00

摘要： The present invention relates to mutants of human growth hormone, which contain newly introduced N-linked or O-linked glycosylation site(s), such that these recombinantly produced polypeptides have glycosylation patterns distinctly different from that of the naturally occurring human growth hormone. The polynucleotide coding sequences for the mutants, expression cassettes comprising the coding sequences, cells expressing the mutants, and methods for producing the mutants are also disclosed. Further disclosed are pharmaceutical compositions comprising the mutants and method for using the mutants.