公开(公告)号：EP3428263A1

公开(公告)日：2019-01-16

申请号：EP16893536.9

申请日：2016-03-11

申请人： Nikon Corporation ,  National Institutes of Biomedical Innovation, Health and Nutrition

发明人： KII Hiroaki ,  KIYOTA Yasujiro ,  UOZUMI Takayuki ,  FURUE Miho ,  SUGA Mika

IPC分类号： C12M1/00 ,  C12Q1/68

摘要： An evaluation device includes a state determination unit which is configured to determine a state of cells which are an observation target under non-standard conditions on the basis of information acquired from an image of cells under standard conditions.

公开(公告)号：EP2418473A1

公开(公告)日：2012-02-15

申请号：EP10761751.6

申请日：2010-04-09

申请人： Nikon Corporation

发明人： KURODA Hideo ,  WATANABE Hirotada ,  WADA Yoichi ,  KIYOTA Yasujiro ,  UOZUMI Takayuki ,  KOSHIBA Ryuji

IPC分类号： G01N21/17 ,  G01N21/64 ,  G02B21/36

CPC分类号： G02B21/367 ,  G01N21/6486 ,  G02B21/16 ,  G02B21/365 ,  G06K9/00134 ,  G06K9/0014

摘要： The present invention relates to a biological specimen observation apparatus whereby observation of a biological specimen can be performed accurately. In macro observation, a biological change region is extracted from a macro image (S32), a micro observation point corresponding to an extracted biological change region is registered (S33), and an object for tracking is identified. In micro observation, it is judged from the micro image whether or not biological change has continued in the biological change region at the micro observation point (S41), and the registered micro observation point is updated on the basis of this judgment result (S42). By this means, it is possible to carry out both macro observation for detecting a biological change region and micro observation for observing the progress of growth of a partial minute region where biological change has been exhibited, and therefore it is possible to carry out accurate observation of a biological specimen. The present invention can be applied to a biological specimen observation apparatus which carries out observation of a biological specimen, for example.

摘要翻译： 本发明涉及一种能够准确地进行生物样本的观察的生物样本观察装置。 在宏观观察中，从宏观图像提取生物变化区域（S32），登记与提取的生物变化区域对应的微观观察点（S33），并且识别跟踪对象。 在微观观察中，从微观图像判断微观察点处的生物变化区域的生物变化是否持续（S41），并根据该判断结果更新登记的微观察点（S42） 。 通过这种方式，可以进行用于检测生物变化区域的宏观观察和用于观察已经展示生物变化的部分微小区域的生长进展的微观察，因此可以进行准确的观察 的生物标本。 本发明可以应用于例如进行生物样本的观察的生物样本观察装置。

公开(公告)号：EP3428262A1

公开(公告)日：2019-01-16

申请号：EP16893530.2

申请日：2016-03-11

申请人： Nikon Corporation

发明人： KII Hiroaki ,  KIYOTA Yasujiro ,  UOZUMI Takayuki ,  YAMAZAKI Yoichi

IPC分类号： C12M1/00

摘要： An image processing device includes an image processing unit that performs image processing on an observed image in which a cell is imaged and an image processing method selector that is configured to determine an observed image processing method for analyzing the imaged cell on the basis of information of a processed image obtained through image processing of the image processing unit.

公开(公告)号：EP2453005A1

公开(公告)日：2012-05-16

申请号：EP10797165.7

申请日：2010-07-08

申请人： Nikon Corporation

发明人： KIYOTA Yasujiro

IPC分类号： C12M1/34

CPC分类号： C12M47/04 ,  C12M23/50 ,  C12M41/46 ,  C12M41/48

摘要： The present invention relates to a cell picking support device, a display device and a culture container that can enhance work efficiency. An adapter 52A fixes a well plate 31A that holds cells cultured in a cell culture observation device. A control unit 71 reads culture observation information that includes cell information and coordinate information, from a memory card that is fitted to an external memory I/F 62a. A display unit 72 is provided on a placing surface side of the well plate 31A that is fixed by the adapter 52A, and in response to control by the control unit 71, displays the cell information, read from the memory card, at display positions corresponding to positions on the bottom face of the well plate 31A, as specified by the coordinate information. The present invention can be used, for instance, as a cell picking support device that supports a picking operation that is performed in a clean bench.

摘要翻译： 本发明涉及能提高工作效率的细胞采集支持装置，显示装置和培养容器。 适配器52A固定保持细胞培养观察装置中培养的细胞的孔板31A。 控制单元71从装配到外部存储器I / F 62a的存储卡读取包括单元信息和坐标信息的文化观察信息。 显示单元72设置在由适配器52A固定的孔板31A的放置表面侧上，并且响应于控制单元71的控制，将从存储卡读取的单元信息显示在相应的显示位置处 到位于孔板31A的底面上的位置，如坐标信息所规定。 例如，本发明可以用作支持在清洁工作台中执行的拾取操作的细胞采集支持装置。

公开(公告)号：EP2453005B1

公开(公告)日：2019-09-04

申请号：EP10797165.7

申请日：2010-07-08

申请人： Nikon Corporation

发明人： KIYOTA Yasujiro

IPC分类号： C12M1/34

公开(公告)号：EP3865565A1

公开(公告)日：2021-08-18

申请号：EP19871996.5

申请日：2019-10-04

申请人： NIKON CORPORATION

发明人： DAN Tomoro ,  KII Hiroaki ,  UOZUMI Takayuki ,  KIYOTA Yasujiro

IPC分类号： C12M1/34 ,  C12Q1/04 ,  G06T7/00

摘要： An image processing device includes: a colony region extraction unit that is configured to extract a colony region of pluripotent stem cells from a microscopic image obtained using transmitted illumination; a high luminance region extraction unit that is configured to extract, from the microscopic image, a high luminance region that is a region on the basis of a group of pixels with larger luminance values than a standard value from among pixels constituting the microscopic image; an extraction target region extraction unit that is configured to extract an extraction target region from the microscopic image on the basis of the colony region extracted by the colony region extraction unit and the high luminance region extracted by the high luminance region extraction unit; and an output unit that is configured to output, as an extraction result, the extraction target region extracted by the extraction target region extraction unit.

公开(公告)号：EP3578633A1

公开(公告)日：2019-12-11

申请号：EP17895201.6

申请日：2017-11-07

申请人： Nikon Corporation

发明人： KOIKE Tetsuya ,  TAKAHASHI Satoshi ,  KIYOTA Yasujiro ,  WATANABE Shunji

IPC分类号： C12M1/34 ,  C12M1/00 ,  G06T7/00

摘要： A culturing assistance device includes an image acquisition unit configured to acquire a captured image of cells during culturing at determined times, a storage control unit configured to store the captured image acquired by the image acquisition unit and event information indicating an event related to culturing of the cells, and a learning unit configured to learn a relationship between the stored captured image and the stored event information.

公开(公告)号：EP3467103A1

公开(公告)日：2019-04-10

申请号：EP17802926.0

申请日：2017-05-26

申请人： Nikon Corporation

发明人： KII Hiroaki ,  KIYOTA Yasujiro ,  UOZUMI Takayuki ,  FURUE Miho ,  SUGA Mika

IPC分类号： C12N5/0797 ,  C12M3/00

摘要： The present invention provides a method for producing neural stem cells, the method including a culture step of culturing cells using a medium that contains a TGF-β receptor inhibitor and a protein kinase C inhibitor. The method for producing neural stem cells is a method that includes a first culture step of culturing cells using a first medium that does not contain a TGF-β receptor inhibitor and a protein kinase C inhibitor, and a second culture step of culturing the cells using a second medium that contains a TGF-β receptor inhibitor and a protein kinase C inhibitor, after the first culture step. The method for producing neural stem cells is a method that includes a first culture step of culturing cells using a first medium that contains ascorbic acid or an ascorbic acid derivative, and a second culture step of culturing the cells using a second medium that contains a TGF-β receptor inhibitor and a protein kinase C inhibitor, after the first culture step.

公开(公告)号：EP3428261A1

公开(公告)日：2019-01-16

申请号：EP16893527.8

申请日：2016-03-11

申请人： Nikon Corporation

发明人： KII Hiroaki ,  KIYOTA Yasujiro ,  UOZUMI Takayuki ,  KAWASHIMA Kazuhiro ,  YAMAZAKI Yoichi

IPC分类号： C12M1/00

摘要： An image processing device includes: an associating unit configured to associate information on an identification element used to identify a cell in imaged cell information with information on a non-identification element serving as an element of the cell which is not the identification element on the basis of the information on the identification element and information of the non-identification element; and a status determination unit configured to determine a cell status on the basis of the information on the identification element and the information on the non-identification element associated together by the linking unit.

公开(公告)号：EP2447752A1

公开(公告)日：2012-05-02

申请号：EP10772187.0

申请日：2010-05-07

申请人： Nikon Corporation

发明人： KIYOTA Yasujiro ,  WADA Yoichi ,  KII Hiroaki

IPC分类号： G02B7/28 ,  G02B7/36 ,  G02B21/00

CPC分类号： G02B21/244 ,  C12M21/06 ,  C12M25/01 ,  C12M41/14 ,  C12M41/36 ,  G02B7/38

摘要： The present invention relates to a focus control apparatus and a culture observation apparatus that enables simple and reliable detection of a focusing position of an objective lens on a subject and with certainty.
When a focusing position of the objective lens on a culture medium drop D11 in a culture container 61 is detected, the culture observation apparatus selects an area, which includes an edge portion of the culture medium drop D11 and does not include an area located within a predetermined distance from the edge of the culture container 61, as an AF area which is to be a target of detecting the focusing position of the objective lens on the culture medium drop D11. Then the culture observation apparatus detects the focusing position on the culture medium drop D11 based on an observation image of the AF area. By detecting the focusing position as a targeting area that is different from an area which is near the edge of the culture container 61 and where illumination unevenness is generated, the detection accuracy can be improved. The present invention can be applied to a culture observation apparatus.

摘要翻译： 本发明涉及一种聚焦控制装置和培养观察装置，其能够确实地简单可靠地检测物镜的聚焦位置。 当培养容器61中的物镜的聚焦位置下降D11被检测到时，培养观察装置选择包括培养液滴D11的边缘部分的区域，不包括位于培养容器61内的区域 距离培养容器61的边缘预定距离，作为将物镜的聚焦位置检测到培养基液滴D11上的AF区域。 然后，文化观察装置基于AF区域的观察图像来检测培养基液滴D11上的聚焦位置。 通过将聚焦位置检测为与培养容器61的边缘附近的区域不同的瞄准区域，并且产生照明不均匀，可以提高检测精度。 本发明可以应用于培养观察装置。