公开(公告)号：EP3428262A1

公开(公告)日：2019-01-16

申请号：EP16893530.2

申请日：2016-03-11

申请人： Nikon Corporation

发明人： KII Hiroaki ,  KIYOTA Yasujiro ,  UOZUMI Takayuki ,  YAMAZAKI Yoichi

IPC分类号： C12M1/00

摘要： An image processing device includes an image processing unit that performs image processing on an observed image in which a cell is imaged and an image processing method selector that is configured to determine an observed image processing method for analyzing the imaged cell on the basis of information of a processed image obtained through image processing of the image processing unit.

公开(公告)号：EP3865565A1

公开(公告)日：2021-08-18

申请号：EP19871996.5

申请日：2019-10-04

申请人： NIKON CORPORATION

发明人： DAN Tomoro ,  KII Hiroaki ,  UOZUMI Takayuki ,  KIYOTA Yasujiro

IPC分类号： C12M1/34 ,  C12Q1/04 ,  G06T7/00

摘要： An image processing device includes: a colony region extraction unit that is configured to extract a colony region of pluripotent stem cells from a microscopic image obtained using transmitted illumination; a high luminance region extraction unit that is configured to extract, from the microscopic image, a high luminance region that is a region on the basis of a group of pixels with larger luminance values than a standard value from among pixels constituting the microscopic image; an extraction target region extraction unit that is configured to extract an extraction target region from the microscopic image on the basis of the colony region extracted by the colony region extraction unit and the high luminance region extracted by the high luminance region extraction unit; and an output unit that is configured to output, as an extraction result, the extraction target region extracted by the extraction target region extraction unit.

公开(公告)号：EP3467103A1

公开(公告)日：2019-04-10

申请号：EP17802926.0

申请日：2017-05-26

申请人： Nikon Corporation

发明人： KII Hiroaki ,  KIYOTA Yasujiro ,  UOZUMI Takayuki ,  FURUE Miho ,  SUGA Mika

IPC分类号： C12N5/0797 ,  C12M3/00

摘要： The present invention provides a method for producing neural stem cells, the method including a culture step of culturing cells using a medium that contains a TGF-β receptor inhibitor and a protein kinase C inhibitor. The method for producing neural stem cells is a method that includes a first culture step of culturing cells using a first medium that does not contain a TGF-β receptor inhibitor and a protein kinase C inhibitor, and a second culture step of culturing the cells using a second medium that contains a TGF-β receptor inhibitor and a protein kinase C inhibitor, after the first culture step. The method for producing neural stem cells is a method that includes a first culture step of culturing cells using a first medium that contains ascorbic acid or an ascorbic acid derivative, and a second culture step of culturing the cells using a second medium that contains a TGF-β receptor inhibitor and a protein kinase C inhibitor, after the first culture step.

公开(公告)号：EP3428261A1

公开(公告)日：2019-01-16

申请号：EP16893527.8

申请日：2016-03-11

申请人： Nikon Corporation

发明人： KII Hiroaki ,  KIYOTA Yasujiro ,  UOZUMI Takayuki ,  KAWASHIMA Kazuhiro ,  YAMAZAKI Yoichi

IPC分类号： C12M1/00

摘要： An image processing device includes: an associating unit configured to associate information on an identification element used to identify a cell in imaged cell information with information on a non-identification element serving as an element of the cell which is not the identification element on the basis of the information on the identification element and information of the non-identification element; and a status determination unit configured to determine a cell status on the basis of the information on the identification element and the information on the non-identification element associated together by the linking unit.

公开(公告)号：EP2447752A1

公开(公告)日：2012-05-02

申请号：EP10772187.0

申请日：2010-05-07

申请人： Nikon Corporation

发明人： KIYOTA Yasujiro ,  WADA Yoichi ,  KII Hiroaki

IPC分类号： G02B7/28 ,  G02B7/36 ,  G02B21/00

CPC分类号： G02B21/244 ,  C12M21/06 ,  C12M25/01 ,  C12M41/14 ,  C12M41/36 ,  G02B7/38

摘要： The present invention relates to a focus control apparatus and a culture observation apparatus that enables simple and reliable detection of a focusing position of an objective lens on a subject and with certainty.
When a focusing position of the objective lens on a culture medium drop D11 in a culture container 61 is detected, the culture observation apparatus selects an area, which includes an edge portion of the culture medium drop D11 and does not include an area located within a predetermined distance from the edge of the culture container 61, as an AF area which is to be a target of detecting the focusing position of the objective lens on the culture medium drop D11. Then the culture observation apparatus detects the focusing position on the culture medium drop D11 based on an observation image of the AF area. By detecting the focusing position as a targeting area that is different from an area which is near the edge of the culture container 61 and where illumination unevenness is generated, the detection accuracy can be improved. The present invention can be applied to a culture observation apparatus.

摘要翻译： 本发明涉及一种聚焦控制装置和培养观察装置，其能够确实地简单可靠地检测物镜的聚焦位置。 当培养容器61中的物镜的聚焦位置下降D11被检测到时，培养观察装置选择包括培养液滴D11的边缘部分的区域，不包括位于培养容器61内的区域 距离培养容器61的边缘预定距离，作为将物镜的聚焦位置检测到培养基液滴D11上的AF区域。 然后，文化观察装置基于AF区域的观察图像来检测培养基液滴D11上的聚焦位置。 通过将聚焦位置检测为与培养容器61的边缘附近的区域不同的瞄准区域，并且产生照明不均匀，可以提高检测精度。 本发明可以应用于培养观察装置。

公开(公告)号：EP3964562A1

公开(公告)日：2022-03-09

申请号：EP20796277.0

申请日：2020-04-23

申请人： NIKON CORPORATION

发明人： ICHIHASHI Toru ,  KII Hiroaki ,  TAKASAKI Tetsuomi ,  UOZUMI Takayuki ,  YAMAZAKI Yoichi ,  KINEHARA Masaki

IPC分类号： C12M1/34 ,  G06T7/00 ,  G06T7/246 ,  C12Q1/02 ,  G01N33/483

摘要： A cell tracking method is a cell tracking method for performing cell tracking on the basis of a plurality of cell images captured in time series, and includes an extraction process of extracting a tracking region corresponding to the cell for each of the plurality of cell images, a position calculation process of calculating a change in a position of the tracking region extracted in the extraction process on the basis of the plurality of cell images and tracking the cell on the basis of the calculated change in the position of the tracking region, a determination process of determining whether or not the cell as a tracking target is in a cell division state on the basis of the cell image, and a stop process of stopping the tracking of the cell when the cell is determined to be in the cell division state in the determination process.

公开(公告)号：EP3865566A1

公开(公告)日：2021-08-18

申请号：EP19872004.7

申请日：2019-10-02

申请人： NIKON CORPORATION ,  Kyoto University

发明人： DAN Tomoro ,  KII Hiroaki ,  UOZUMI Takayuki ,  SATO Yoshiko ,  YOSHIDA Shinsuke

IPC分类号： C12M1/34

摘要： A passage timing calculation device includes a time change calculating section configured to calculate a time change in an area occupied by an extraction target region that is a region in which a striated pattern appears from a plurality of microscope images of pluripotent stem cells captured at different times, a change point detecting section configured to detect a change point of the time change in an area occupied by the extraction target region, and a passage timing calculating section configured to determine a passage timing of the pluripotent stem cells on the basis of the change point.

公开(公告)号：EP3428263A1

公开(公告)日：2019-01-16

申请号：EP16893536.9

申请日：2016-03-11

申请人： Nikon Corporation ,  National Institutes of Biomedical Innovation, Health and Nutrition

发明人： KII Hiroaki ,  KIYOTA Yasujiro ,  UOZUMI Takayuki ,  FURUE Miho ,  SUGA Mika

IPC分类号： C12M1/00 ,  C12Q1/68

摘要： An evaluation device includes a state determination unit which is configured to determine a state of cells which are an observation target under non-standard conditions on the basis of information acquired from an image of cells under standard conditions.