公开(公告)号：EP2857837B1

公开(公告)日：2018-07-25

申请号：EP13798097.5

申请日：2013-05-31

申请人： SD Biosensor Inc.

发明人： CHOI, Hyoung Gil ,  HONG, Sung Yub ,  HWANG, Hee Young ,  LEE, Hyo Keun

IPC分类号： G01N33/558 ,  G01N33/532 ,  G01N33/543

CPC分类号： G01N33/5436 ,  G01N33/49 ,  G01N33/532 ,  G01N33/538 ,  G01N33/558 ,  G01N33/723 ,  G01N33/90

摘要： The present invention relates to a freeze-dried conjugate structure for immunochromatography, an immunoassay kit comprising the freeze-dried conjugate structure and an immunochromatographic strip, and a method for qualitatively or quantitatively analyzing an analyte in a sample using the immunoassay kit. Because a sample is subjected to immunochromatography after reacting uniformly with a freeze-dried conjugate structure separately prepared according to the present invention externally, quantitative analysis can be performed with high reproducibility and linearity depending on a concentration compared to a conventional assay method which is performed using an immunochromatographic strip comprising a conjugate pad prepared by adsorbing a conjugate. In addition, the freeze-dried conjugate structure of the present invention can be provided in a state in which it is received in a chamber additionally comprising a cap. Thus, it can be stored without contamination and is easy to carry. In addition, the freeze-dried conjugate structure can be rapidly and uniformly dissolved so that it is immediately allowed to react with a mixture of a buffer and a sample and the reaction product can be analyzed by an immunoassay kit. Thus, it is suitable for use in point-of-care testing.

公开(公告)号：EP2857837A1

公开(公告)日：2015-04-08

申请号：EP13798097.5

申请日：2013-05-31

申请人： SD Biosensor Inc.

发明人： CHOI, Hyoung Gil ,  HONG, Sung Yub ,  HWANG, Hee Young ,  LEE, Hyo Keun

IPC分类号： G01N33/53 ,  G01N33/532 ,  G01N33/538 ,  G01N30/02

CPC分类号： G01N33/5436 ,  G01N33/49 ,  G01N33/532 ,  G01N33/538 ,  G01N33/558 ,  G01N33/723 ,  G01N33/90

摘要： The present invention relates to a freeze-dried conjugate structure for immunochromatography, an immunoassay kit comprising the freeze-dried conjugate structure and an immunochromatographic strip, and a method for qualitatively or quantitatively analyzing an analyte in a sample using the immunoassay kit.
Because a sample is subjected to immunochromatography after reacting uniformly with a freeze-dried conjugate structure separately prepared according to the present invention externally, quantitative analysis can be performed with high reproducibility and linearity depending on a concentration compared to a conventional assay method which is performed using an immunochromatographic strip comprising a conjugate pad prepared by adsorbing a conjugate.
In addition, the freeze-dried conjugate structure of the present invention can be provided in a state in which it is received in a chamber additionally comprising a cap. Thus, it can be stored without contamination and is easy to carry. In addition, the freeze-dried conjugate structure can be rapidly and uniformly dissolved so that it is immediately allowed to react with a mixture of a buffer and a sample and the reaction product can be analyzed by an immunoassay kit. Thus, it is suitable for use in point-of-care testing.

摘要翻译： 本发明涉及一种冷冻干燥的共轭结构的免疫层析，在免疫测定试剂盒，其包括冷冻干燥的共轭结构和到免疫层析试纸条，以及用于使用所述免疫测定试剂盒的样品中定性或定量分析的分析物的方法。 因为一个样品进行与冷冻干燥的共轭结构一致反应之后，以免疫色谱法分别制备雅丁到本发明的外部，定量分析能够以高再现性和线性度依赖于浓度相比于常规测定法所有的其使用执行的被执行的 在免疫层析条，其包括通过吸附的缀合物制备的偶联物垫。 另外，可以在其中它是在一个腔室接收另外包括盖的状态下被设置在本发明的冷冻干燥的共轭结构。 因此，它可以存储没有污染，而且便于携带。 此外，冷冻干燥的共轭结构可以是快速且均匀地溶解，以便做它立即使其与和该反应产物的缓冲液的混合物的样品和反应可以通过在免疫测定试剂盒进行分析。 因此，适用于床边血糖检测使用。