公开(公告)号：EP2489743A2

公开(公告)日：2012-08-22

申请号：EP12160299.9

申请日：2008-07-17

申请人： Somalogic, Inc.

发明人： Zichi, Dominic ,  Wilcox, Sheri K. ,  Bock, Chris ,  Schneider, Daniel J. ,  Eaton, Bruce ,  Gold, Larry

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6811 ,  C12N15/1048 ,  C12N15/111 ,  C12N15/115 ,  C12N2310/16 ,  C12N2320/13 ,  C12Q2541/101 ,  C12Q2525/205 ,  C12Q2525/117

摘要： The present disclosure describes improved SELEX methods for producing aptamers that are capable of binding to target molecules and improved photoSELEX methods for producing photo reactive aptamers that are capable of both binding and covalently cross linking to target molecules. Specifically, the present disclosure describes methods for producing aptamers and photoaptamers having slower dissociation rate constants than are obtained using prior SELEX and photoSELEX methods. The disclosure further describes aptamers and photoaptamers having slower dissociation rate constants than those obtained using prior methods. In addition, the disclosure describes aptamer constructs that include a variety of functionalities, including a cleavable element, a detection element, and a capture or immobilization element.

摘要翻译： 本公开描述了用于产生能够结合靶分子的适配体的改进的SELEX方法，以及用于产生能够结合和共价交联到靶分子的光反应性适体的改进的光学光学法。 具体地，本公开描述了用于生产具有比使用先前的SELEX和photoSELEX方法获得的解离速率常数更慢的适体和光致抗体的方法。 本公开进一步描述了具有比使用现有方法获得的那些更快的解离速率常数的适体和光致抗体。 此外，本公开描述了包括多种功能的适体构建体，包括可切割元件，检测元件和捕获或固定元件。

公开(公告)号：EP2626436A3

公开(公告)日：2013-10-16

申请号：EP13164719.0

申请日：2008-07-17

申请人： Somalogic, Inc.

发明人： Schneider, Daniel J. ,  Nieuwlandt, Dan ,  Eaton, Bruce ,  Stanton, Marty ,  Gupta, Shashi ,  Kraemer, Stephan ,  Zichi, Dominic ,  Gold, Larry

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6811 ,  C12N15/1048 ,  C12N15/111 ,  C12N15/115 ,  C12N2310/16 ,  C12N2320/13 ,  C12Q2541/101 ,  C12Q2525/205 ,  C12Q2525/117

摘要： The present disclosure describes methods, devices, reagents, and kits for the detection of one or more target molecules that may be present in a test sample. The described methods, devices, kits, and reagents facilitate the detection and quantification of a non-nucleic acid target (e.g., a protein target) in a test sample by detecting and quantifying a nucleic acid (i.e., an aptamer). The methods described create a nucleic acid surrogate for a non-nucleic acid target, thus allowing the wide variety of nucleic acid technologies, including amplification, to be applied to a broader range of desired targets, especially protein targets. The disclosure further describes aptamer constructs that facilitate the use of aptamers in a variety of analytical detection applications.

公开(公告)号：EP2172566A1

公开(公告)日：2010-04-07

申请号：EP09012809.1

申请日：2008-07-17

申请人： Somalogic, Inc.

发明人： Zichi, Dominic ,  Wilcox, Sheri K. ,  Bock, Chris ,  Schneider, Daniel J. ,  Eaton, Bruce ,  Gold, Larry

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6811 ,  C12N15/1048 ,  C12N15/111 ,  C12N15/115 ,  C12N2310/16 ,  C12N2320/13 ,  C12Q2541/101 ,  C12Q2525/205 ,  C12Q2525/117

摘要： The present disclosure describes improved SELEX methods for producing aptamers that are capable of binding to target molecules and improved photoSELEX methods for producing photoreactive aptamers that are capable of both binding and covalently cross linking to target molecules. Specifically, the present disclosure describes methods for producing aptamers and photoaptamers having slower dissociation rate constants than are obtained using prior SELEX and photoSELEX methods. The disclosure further describes aptamers and photoaptamers having slower dissociation rate constants than those obtained using prior methods. In addition, the disclosure describes aptamer constructs that include a variety of functionalities, including a cleavable element, a detection element, and a capture or immobilization element.

摘要翻译： 本公开描述了用于产生能够结合靶分子的适配体的改进的SELEX方法，以及用于产生能够结合和共价交联到靶分子的光反应性适体的改进的光学光学方法。 具体地，本公开描述了用于生产具有比使用先前的SELEX和photoSELEX方法获得的解离速率常数更慢的适体和光致抗体的方法。 本公开进一步描述了具有比使用现有方法获得的那些更快的解离速率常数的适体和光致抗体。 此外，本公开描述了包括多种功能的适体构建体，包括可切割元件，检测元件和捕获或固定元件。

公开(公告)号：EP2172566B1

公开(公告)日：2015-03-04

申请号：EP09012809.1

申请日：2008-07-17

申请人： Somalogic, Inc.

发明人： Zichi, Dominic ,  Wilcox, Sheri K. ,  Bock, Chris ,  Schneider, Daniel J. ,  Eaton, Bruce ,  Gold, Larry

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6811 ,  C12N15/1048 ,  C12N15/111 ,  C12N15/115 ,  C12N2310/16 ,  C12N2320/13 ,  C12Q2541/101 ,  C12Q2525/205 ,  C12Q2525/117

公开(公告)号：EP2336314A1

公开(公告)日：2011-06-22

申请号：EP10176864.6

申请日：2008-07-17

申请人： Somalogic, Inc.

发明人： Schneider, Daniel J. ,  Wilcox, Sheri K. ,  Zichi, Dominic ,  Nieuwlandt, Dan ,  Carter, Jeff ,  Gold, Larry

IPC分类号： C12N15/10

CPC分类号： C12Q1/6811 ,  C12N15/1048 ,  C12N15/111 ,  C12N15/115 ,  C12N2310/16 ,  C12N2320/13 ,  C12Q2541/101 ,  C12Q2525/205 ,  C12Q2525/117

摘要： The present disclosure describes improved SELEX methods for generating nucleic acid ligands that are capable of binding to target molecules and improved photoSELEX methods for generating photoreactive nucleic acid ligands that are capable of both binding and covalently crosslinking to target molecules. The disclosure further describes nucleic acid libraries having expanded physical and chemical properties and their use in SELEX and photoSELEX; methods for increasing the crosslinking efficiencies of photoaptamers; methods for producing photoaptamers having selective modifications that enhance functionality and minimize non-specific photoreactions; and methods for generating truncated nucleic acid ligands from nucleic acid ligands of longer length. The disclosure further describes aptamers and photoaptamers obtained by using any of the foregoing.

摘要翻译： 本公开描述了用于产生能够结合靶分子的核酸配体的改进的SELEX方法和用于产生能够与靶分子结合和共价交联的光反应性核酸配体的改进的光SIMLEX方法。 本公开进一步描述具有扩展的物理和化学性质及其在SELEX和photoSELEX中的用途的核酸文库; 提高光催化剂交联效率的方法; 制备具有增强功能并使非特异性光反应最小化的选择性修饰的光饱和物的方法; 以及用于从较长长度的核酸配体产生截短的核酸配体的方法。 本公开进一步描述了通过使用上述任何一种获得的适体和受精胺。

公开(公告)号：EP2952894A1

公开(公告)日：2015-12-09

申请号：EP15158380.4

申请日：2007-01-16

申请人： Somalogic, Inc.

发明人： Heil, James R. ,  Schneider, Daniel J. ,  Nieuwlandt, Daniel T. ,  Wilcox, Sheri. K ,  Zichi, Dominic ,  Gander, Todd ,  Eaton, Bruce ,  Gold, Larry

IPC分类号： G01N33/53 ,  C12N15/115

CPC分类号： G01N33/5308 ,  C12N15/115 ,  C12N2310/16 ,  C12N2310/3519 ,  C12Q1/6816 ,  C12Q1/6832 ,  C12Q1/6834 ,  G01N33/58 ,  C12Q2565/514 ,  C12Q2525/205 ,  C12Q2525/161 ,  C12Q2537/101 ,  C12Q2523/313

摘要： The present disclosure describes methods, devices, reagents, and kits for the detection of one or more target molecules that may be present in a test sample. In one embodiment, a test sample is contacted with an aptamer that includes a tag and has a specific affinity for a target molecule. An aptamer affinity complex that includes an aptamer bound to its target molecule is allowed to form. If the test sample contains the target molecule, an aptamer affinity complex will generally form in the test sample. The aptamer affinity complex is optionally converted to an aptamer covalent complex that includes an aptamer covalently bound to its target molecule. The aptamer affinity complex (or optional aptamer covalent complex) can then be detected and/or quantified using any of a variety of methods known to one skilled in the art, including using a solid support, using mass spectrometry, and using quantitative polymerase chain reaction (Q-PCR).

摘要翻译： 本公开描述了用于检测可能存在于测试样品中的一种或多种靶分子的方法，装置，试剂和试剂盒。 在一个实施方案中，将测试样品与包含标签的适配体接触并对靶分子具有特异性亲和力。 允许形成包含与其靶分子结合的适体的适体亲和复合体。 如果测试样品含有目标分子，通常会在测试样品中形成适体亲和复合体。 适体亲和复合物任选地转化为适体共价复合物，其包括与其靶分子共价结合的适体。 然后可以使用本领域技术人员已知的多种方法中的任何一种来检测和/或定量适体亲和性复合物（或任选的适体共价复合物），包括使用固体支持物，使用质谱法和使用定量聚合酶链反应 （Q-PCR）。

公开(公告)号：EP2933340A1

公开(公告)日：2015-10-21

申请号：EP15152547.4

申请日：2008-07-17

申请人： Somalogic, Inc.

发明人： Zichi, Dominic ,  Wilcox, Sheri K. ,  Bock, Chris ,  Schneider, Daniel J. ,  Eaton, Bruce ,  Gold, Larry

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6811 ,  C12N15/1048 ,  C12N15/111 ,  C12N15/115 ,  C12N2310/16 ,  C12N2320/13 ,  C12Q2541/101 ,  C12Q2525/205 ,  C12Q2525/117

摘要： The present disclosure describes improved SELEX methods for producing aptamers that are capable of binding to target molecules and improved photoSELEX methods for producing photoreactive aptamers that are capable of both binding and covalently cross linking to target molecules. Specifically, the present disclosure describes methods for producing aptamers and photoaptamers having slower dissociation rate constants than are obtained using prior SELEX and photoSELEX methods. The disclosure further describes aptamers and photoaptamers having slower dissociation rate constants than those obtained using prior methods. In addition, the disclosure describes aptamer constructs that include a variety of functionalities, including a cleavable element, a detection element, and a capture or immobilization element.

摘要翻译： 本公开描述了用于产生能够结合靶分子的适配体的改进的SELEX方法，以及用于产生能够结合和共价交联到靶分子的光反应性适体的改进的光学光学方法。 具体地，本公开描述了用于生产具有比使用先前的SELEX和photoSELEX方法获得的解离速率常数更慢的适体和光致抗体的方法。 本公开进一步描述了具有比使用现有方法获得的那些更快的解离速率常数的适体和光致抗体。 此外，本公开描述了包括多种功能的适体构建体，包括可切割元件，检测元件和捕获或固定元件。

公开(公告)号：EP2613147A1

公开(公告)日：2013-07-10

申请号：EP13158346.0

申请日：2007-01-16

申请人： Somalogic, Inc.

发明人： Heil, James R. ,  Schneider, Daniel J. ,  Nieuwlandt, Daniel T. ,  Wilcox, Sheri K. ,  Zichi, Dominic ,  Gander, Todd ,  Eaton, Bruce ,  Gold, Larry

IPC分类号： G01N33/53

CPC分类号： G01N33/5308 ,  C12N15/115 ,  C12N2310/16 ,  C12N2310/3519 ,  C12Q1/6816 ,  C12Q1/6832 ,  C12Q1/6834 ,  G01N33/58 ,  C12Q2565/514 ,  C12Q2525/205 ,  C12Q2525/161 ,  C12Q2537/101 ,  C12Q2523/313

摘要： The present disclosure describes methods, devices, reagents, and kits for the detection of one or more target molecules that may be present in a test sample. In one embodiment, a test sample is contacted with an aptamer that includes a tag and has a specific affinity for a target molecule. An aptamer affinity complex that includes an aptamer bound to its target molecule is allowed to form. If the test sample contains the target molecule, an aptamer affinity complex will generally form in the test sample. The aptamer affinity complex is optionally converted to an aptamer covalent complex that includes an aptamer covalently bound to its target molecule. The aptamer affinity complex (or optional aptamer covalent complex) can then be detected and/or quantified using any of a variety of methods known to one skilled in the art, including using a solid support, using mass spectrometry, and using quantitative polymerase chain reaction (Q-PCR).

摘要翻译： 本公开描述了用于检测可能存在于测试样品中的一种或多种靶分子的方法，装置，试剂和试剂盒。 在一个实施方案中，将测试样品与包含标签的适配体接触并对靶分子具有特异性亲和力。 允许形成包含与其靶分子结合的适体的适体亲和复合体。 如果测试样品含有目标分子，通常会在测试样品中形成适体亲和复合体。 适体亲和复合物任选地转化为适体共价复合物，其包括与其靶分子共价结合的适体。 然后可以使用本领域技术人员已知的多种方法中的任何一种来检测和/或定量适体亲和性复合物（或任选的适体共价复合物），包括使用固体支持物，使用质谱法和使用定量聚合酶链反应 （Q-PCR）。

公开(公告)号：EP2933340B1

公开(公告)日：2017-09-06

申请号：EP15152547.4

申请日：2008-07-17

申请人： Somalogic, Inc.

发明人： Zichi, Dominic ,  Wilcox, Sheri K. ,  Bock, Chris ,  Schneider, Daniel J. ,  Eaton, Bruce ,  Gold, Larry

IPC分类号： C12N15/115

CPC分类号： C12Q1/6811 ,  C12N15/1048 ,  C12N15/111 ,  C12N15/115 ,  C12N2310/16 ,  C12N2320/13 ,  C12Q2541/101 ,  C12Q2525/205 ,  C12Q2525/117

公开(公告)号：EP2952894B1

公开(公告)日：2017-08-30

申请号：EP15158380.4

申请日：2007-01-16

申请人： Somalogic, Inc.

发明人： Heil, James R. ,  Schneider, Daniel J. ,  Nieuwlandt, Daniel T. ,  Wilcox, Sheri. K ,  Zichi, Dominic ,  Gander, Todd ,  Eaton, Bruce ,  Gold, Larry

IPC分类号： G01N33/53 ,  C12N15/115

CPC分类号： G01N33/5308 ,  C12N15/115 ,  C12N2310/16 ,  C12N2310/3519 ,  C12Q1/6816 ,  C12Q1/6832 ,  C12Q1/6834 ,  G01N33/58 ,  C12Q2565/514 ,  C12Q2525/205 ,  C12Q2525/161 ,  C12Q2537/101 ,  C12Q2523/313