公开(公告)号：EP2626436A3

公开(公告)日：2013-10-16

申请号：EP13164719.0

申请日：2008-07-17

申请人： Somalogic, Inc.

发明人： Schneider, Daniel J. ,  Nieuwlandt, Dan ,  Eaton, Bruce ,  Stanton, Marty ,  Gupta, Shashi ,  Kraemer, Stephan ,  Zichi, Dominic ,  Gold, Larry

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6811 ,  C12N15/1048 ,  C12N15/111 ,  C12N15/115 ,  C12N2310/16 ,  C12N2320/13 ,  C12Q2541/101 ,  C12Q2525/205 ,  C12Q2525/117

摘要： The present disclosure describes methods, devices, reagents, and kits for the detection of one or more target molecules that may be present in a test sample. The described methods, devices, kits, and reagents facilitate the detection and quantification of a non-nucleic acid target (e.g., a protein target) in a test sample by detecting and quantifying a nucleic acid (i.e., an aptamer). The methods described create a nucleic acid surrogate for a non-nucleic acid target, thus allowing the wide variety of nucleic acid technologies, including amplification, to be applied to a broader range of desired targets, especially protein targets. The disclosure further describes aptamer constructs that facilitate the use of aptamers in a variety of analytical detection applications.

公开(公告)号：EP2626436A2

公开(公告)日：2013-08-14

申请号：EP13164719.0

申请日：2008-07-17

申请人： Somalogic, Inc.

发明人： Schneider, Daniel J. ,  Nieuwlandt, Dan ,  Eaton, Bruce ,  Stanton, Marty ,  Gupta, Shashi ,  Kraemer, Stephan ,  Zichi, Dominic ,  Gold, Larry

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6811 ,  C12N15/1048 ,  C12N15/111 ,  C12N15/115 ,  C12N2310/16 ,  C12N2320/13 ,  C12Q2541/101 ,  C12Q2525/205 ,  C12Q2525/117

摘要： The present disclosure describes methods, devices, reagents, and kits for the detection of one or more target molecules that may be present in a test sample. The described methods, devices, kits, and reagents facilitate the detection and quantification of a non-nucleic acid target (e.g., a protein target) in a test sample by detecting and quantifying a nucleic acid (i.e., an aptamer). The methods described create a nucleic acid surrogate for a non-nucleic acid target, thus allowing the wide variety of nucleic acid technologies, including amplification, to be applied to a broader range of desired targets, especially protein targets. The disclosure further describes aptamer constructs that facilitate the use of aptamers in a variety of analytical detection applications.

摘要翻译： 本公开描述了用于检测可能存在于测试样品中的一种或多种目标分子的方法，装置，试剂和试剂盒。 所描述的方法，装置，试剂盒和试剂通过检测和定量核酸（即，适体）来促进对测试样品中的非核酸靶标（例如，蛋白质靶标）的检测和定量。 所描述的方法产生非核酸靶标的核酸替代物，因此允许将各种各样的核酸技术（包括扩增）应用于更广泛范围的期望靶标，特别是蛋白质靶标。 本公开进一步描述了适配体构建体，其有助于在各种分析检测应用中使用适配体。

公开(公告)号：EP2336314A1

公开(公告)日：2011-06-22

申请号：EP10176864.6

申请日：2008-07-17

申请人： Somalogic, Inc.

发明人： Schneider, Daniel J. ,  Wilcox, Sheri K. ,  Zichi, Dominic ,  Nieuwlandt, Dan ,  Carter, Jeff ,  Gold, Larry

IPC分类号： C12N15/10

CPC分类号： C12Q1/6811 ,  C12N15/1048 ,  C12N15/111 ,  C12N15/115 ,  C12N2310/16 ,  C12N2320/13 ,  C12Q2541/101 ,  C12Q2525/205 ,  C12Q2525/117

摘要： The present disclosure describes improved SELEX methods for generating nucleic acid ligands that are capable of binding to target molecules and improved photoSELEX methods for generating photoreactive nucleic acid ligands that are capable of both binding and covalently crosslinking to target molecules. The disclosure further describes nucleic acid libraries having expanded physical and chemical properties and their use in SELEX and photoSELEX; methods for increasing the crosslinking efficiencies of photoaptamers; methods for producing photoaptamers having selective modifications that enhance functionality and minimize non-specific photoreactions; and methods for generating truncated nucleic acid ligands from nucleic acid ligands of longer length. The disclosure further describes aptamers and photoaptamers obtained by using any of the foregoing.

摘要翻译： 本公开描述了用于产生能够结合靶分子的核酸配体的改进的SELEX方法和用于产生能够与靶分子结合和共价交联的光反应性核酸配体的改进的光SIMLEX方法。 本公开进一步描述具有扩展的物理和化学性质及其在SELEX和photoSELEX中的用途的核酸文库; 提高光催化剂交联效率的方法; 制备具有增强功能并使非特异性光反应最小化的选择性修饰的光饱和物的方法; 以及用于从较长长度的核酸配体产生截短的核酸配体的方法。 本公开进一步描述了通过使用上述任何一种获得的适体和受精胺。