-
公开(公告)号：EP0744894A1
公开(公告)日：1996-12-04
申请号：EP95909296.0
申请日：1995-01-27
发明人： CANTOR, Charles, R. , NIEMEYER, Christof, M. , SMITH, Cassandra, L. , SANO, Takeshi , HNATOWICH, Donald, J. , RUSCKOWSKI, Mary
IPC分类号： G01N33 , A61K38 , A61K39 , A61K45 , A61K47 , A61K49 , A61K51 , A61P31 , A61P35 , C03C17 , C07H21 , C12N15 , C12Q1
CPC分类号： B82Y5/00 , A61K47/55 , A61K47/557 , A61K47/6949 , A61K51/1268 , C03C17/30 , C03C17/3405 , C12Q1/6804 , C12Q1/682 , Y10S977/80 , Y10S977/808 , Y10S977/898 , Y10S977/906 , Y10S977/92
摘要： The invention is directed to constructs and compositions containing multimeric forms of nucleic acid. Multimeric nucleic acids comprise single-stranded nucleic acids attached via biotin to streptavidin and bound with a functional group. These constructs can be utilized in vivo to treat or identify diseased tissue or cells. Repeated administrations of multimeric nucleic acid compositions produce a rapid and specific amplification of nucleic acid constructs and their attached functional groups. For treatment purposes, functional groups may be toxins, radioisotopes, genes or enzymes. Diagnostically, labeled multimeric constructs may be used to identify specific targets in vivo or in vitro. Multimeric nucleic acids may also be used in nanotechnology and to create self-assembling polymeric aggregates such as membranes of defined porosity, microcircuits and many other products.
-
公开(公告)号：EP1587946B1
公开(公告)日：2009-07-08
申请号：EP04703049.9
申请日：2004-01-16
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6827 , C12Q1/6858 , C12Q2600/156 , C12Q2527/137 , C12Q2537/143 , C12Q2565/627
摘要： The present invention provides an efficient way for high throughput haplotype analysis. Several polymorphic nucleic acid markers, such as SNPs, can be simultaneously and reliably determined through multiplex PCR of single nucleic acid molecules in several parallel single molecule dilutions and the consequent statistical analysis of the results from these parallel single molecule multiplex PCR reactions results in reliable determination of haplotypes present in the subject. The nucleic acid markers can be of any distance to each other on the chromosome. In addition, an approach wherein overlapping DNA markers are analyzed can be used to link smaller haplotypes into larger haplotypes. Consequently, the invention provides a powerful new tool for diagnostic haplotyping and identifying novel haplotypes.
-
公开(公告)号：EP1954828A2
公开(公告)日：2008-08-13
申请号：EP06827001.6
申请日：2006-10-27
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6816 , C12Q1/682 , C12Q2563/107 , C12Q2561/107 , C12Q2522/101
摘要： The present invention relates to a method to detect nucleic acid molecules, such as RNA molecules in vivo using real time protein complementation methods. The invention further relates to methods for detecting nucleic acids, for example RNA in real-time in living cells with a high sensitivity, using a novel split biomolecular conjugate of the invention.
摘要翻译： 本发明涉及使用实时蛋白质互补方法在体内检测核酸分子（例如RNA分子）的方法。 本发明进一步涉及使用本发明的新型分裂生物分子偶联物，以高灵敏度在活细胞中实时检测核酸，例如RNA的方法。
-
公开(公告)号：EP0827551A2
公开(公告)日：1998-03-11
申请号：EP96921212.0
申请日：1996-05-20
IPC分类号： C12Q1
CPC分类号： C12Q1/6827 , C12Q1/6823 , C12Q2537/1376 , C12Q2521/307 , C12Q2535/131 , C12Q2525/151 , C12Q2565/501
摘要： The invention relates to methods for rapidly determining the sequence and/or length of a target sequence. The target sequence may be a series of known or unknown repeat sequences which are hybridized to an array of probes. The hybridized array is digested with a single-strand nuclease and free 3'-hydroxyl groups extended with a nucleic acid polymerase. Nuclease cleaved heteroduplexes can be easily distinguished from nuclease uncleaved heteroduplexes by differential labeling. Probes and target can be differentially labeled with detectable labels. Matched target can be detected by cleaving resulting loops from the hybridized target and creating free 3-hydroxyl groups. These groups are recognized and extended by polymerases added into the reaction system which also adds or releases one label into solution. Analysis of the resulting products using either solid phase or solution. These methods can be used to detect characteristic nucleic acid sequences, to determine target sequence and to screen for genetic defects and disorders. Assays can be conducted on solid surfaces allowing for multiple reactions to be conducted in parallel and, if desired, automated.
-
公开(公告)号：EP1721014B1
公开(公告)日：2013-07-17
申请号：EP05807622.5
申请日：2005-02-18
CPC分类号： C12Q1/6827 , C12Q2600/156 , C12Q2525/186 , C12Q2535/125 , C12Q2565/627 , C12Q2545/101
-
公开(公告)号：EP1546385A2
公开(公告)日：2005-06-29
申请号：EP03752087.1
申请日：2003-09-05
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6851 , C12Q2545/107 , C12Q2535/125 , C12Q2525/186
摘要： The present invention relates to a method for measuring the amount of a target nucleic acid in a sample using a standard which is designed to have one base difference compared with the gene of interest or a 'target nucleic acid sequence.' Use of such standard in combination with a method of 'enhancing' the difference in the standard and the test nucleic acid sample using, for example, a base extension reaction carried right at the mutation site allowing amplification of the standard and target nucleic acids with the same efficiency and facilitating quantification of the target nucleic acid. Thereafter a means of quantifying the 'enhanced' standard and target nucleic acid samples is used to determine the amount of the target nucleic acid. In the preferred embodiment, the quantification means is Mass Spectrometry.
-
7.发明公开ACTIVATED SPLIT-POLYPEPTIDES AND METHODS FOR THEIR PRODUCTION AND USE 审中-公开对于使用活性炭SPALTPOLYPEPTIDE和方法以及
公开(公告)号：EP1948825A2
公开(公告)日：2008-07-30
申请号：EP06827063.6
申请日：2006-10-27
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6883
摘要： The present invention relates to a method to produce activated split-polypeptide fragments that on reconstitution immediately forms an active protein. The method relate to real-time protein complementation. Also encompassed in the invention is a method to split and produce split-fluorescent proteins in an active state which produce a fluorescent signal immediately on reconstitution. The present application also provides methods to detect nucleic acids; non-nucleic acid analytes and nucleic acid hybridization in real-time using the novel activated split-polypeptide fragments of the invention.
-
公开(公告)号：EP1721014A2
公开(公告)日：2006-11-15
申请号：EP05807622.5
申请日：2005-02-18
CPC分类号： C12Q1/6827 , C12Q2600/156 , C12Q2525/186 , C12Q2535/125 , C12Q2565/627 , C12Q2545/101
摘要： The present invention is directed to a method for detecting and quantifying rare mutations in a nucleic acid sample. The nucleic acid molecules under investigation can be either DNA or RNA. The rare mutation can be any type of functional or non-functional nucleic acid change or mutation, such as deletion, insertion, translocation, inversion, one or more base substitution or polymorphism. Therefore, the methods of the present invention are useful in detection of rare mutations in, for example, diagnostic, prognostic and follow-up applications, when the targets are rare known nucleic acid variants mixed in with the wildtype or the more common nucleic acid variant(s).
-
公开(公告)号：EP1587946A2
公开(公告)日：2005-10-26
申请号：EP04703049.9
申请日：2004-01-16
IPC分类号： C12Q1/00
CPC分类号： C12Q1/6827 , C12Q1/6858 , C12Q2600/156 , C12Q2527/137 , C12Q2537/143 , C12Q2565/627
摘要： The present invention provides an efficient way for high throughput haplotype analysis. Several polymorphic nucleic acid markers, such as SNPs, can be simultaneously and reliably determined through multiplex PCR of single nucleic acid molecules in several parallel single molecule dilutions and the consequent statistical analysis of the results from these parallel single molecule multiplex PCR reactions results in reliable determination of haplotypes present in the subject. The nucleic acid markers can be of any distance to each other on the chromosome. In addition, an approach wherein overlapping DNA markers are analyzed can be used to link smaller haplotypes into larger haplotypes. Consequently, the invention provides a powerful new tool for diagnostic haplotyping and identifying novel haplotypes.
-
公开(公告)号：EP0977770A1
公开(公告)日：2000-02-09
申请号：EP98909164.0
申请日：1998-03-13
CPC分类号： C07K14/36
摘要： Compounds and methods are described for producing streptavidin mutants with changed affinities. In particular, modifications to the sequence of the natural streptavidin gene is described to create amino acid substitutions resulting in greater affinity for biotin subsitutes than for biotin.
-
-
-
-
-
-
-
-
-
搜索结果
20 条记录 (耗时 0.021 秒)
