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7 条记录 (耗时 0.013 秒)

    Process for quantitative determination of substrate treated with oxidase
    2.
    发明公开
    Process for quantitative determination of substrate treated with oxidase 失效
    维他命Zur定量Bestimmung eines mit氧化酶behandelten基材。

    公开(公告)号：EP0100217A2

    公开(公告)日：1984-02-08

    申请号：EP83304262.5

    申请日：1983-07-22

    申请人： WAKO PURE CHEMICAL INDUSTRIES, LTD.

    发明人： Sakata, Yoshitsugu ,  Miyashita, Yoshinobu ,  Hamanaka, Tadashi ,  Kodera, Hiroyuki ,  Miki, Yutaka ,  Yamanishi, Kazuhiko ,  Hanada, Toshiro

    IPC分类号： C12Q1/26

    CPC分类号： C12Q1/62 ,  C12Q1/26 ,  C12Q1/54 ,  C12Q1/60

    摘要： A substrate in a sample can be determined quantitatively by measuring colorimetrically superoxide ion generated by treating the sample with a specific oxidase corresponding to the substrate to be determined, said oxidase treatment and measuring of the generated superoxide ion being conducted by using a reagent composition comprising (a) an oxidase, (b) peroxidase, (c) a phenol and/or an amine, (d) a thiol compound, (e) a color producing reagent to be reduced, and if necessary (f) a chelating agent. Further, the peroxidase can be replaced by (b') iron complex of porphyrin or ion chelate of complexane.

    摘要翻译： 样品中的底物可以通过测量通过用对应于待测定底物的特定氧化酶处理样品而产生的比色超氧化物离子来测定，所述氧化酶处理和所测得的超氧化物离子是通过使用包含（ a）氧化酶，（b）过氧化物酶，（c）苯酚和/或胺，（d）硫醇化合物，（e）待还原的着色剂，以及必要时（f）螯合剂。 此外，过氧化物酶可以由（b min）卟啉的铁络合物或络合物的离子螯合物代替。

    Method for measuring an amount of LDL-cholesterol
    4.
    发明公开
    Method for measuring an amount of LDL-cholesterol 失效
    Verfahren zur Messung eines吸入低密度脂蛋白胆固醇

    公开(公告)号：EP0821239A2

    公开(公告)日：1998-01-28

    申请号：EP97112537.2

    申请日：1997-07-22

    申请人： WAKO PURE CHEMICAL INDUSTRIES, LTD

    发明人： Miki, Yutaka ,  Imajo, Nobuko ,  Hanada, Toshiro

    IPC分类号： G01N33/92 ,  C12Q1/60

    CPC分类号： G01N33/92 ,  G01N2800/044 ,  Y10S435/975

    摘要： The present invention is to provide a method for measuring an amount of cholesterol in low density lipoproteins (LDL-cholesterol) in a sample specifically at high accuracy and a reagent used in this method, and the present invention can attain such effect that direct measuring an amount of LDL-cholesterol by widely used automatic analyzers can be conducted by using the invention, which has not been possible after known methods.

    摘要翻译： 本发明提供一种以高精度测定样品中的低密度脂蛋白（LDL-胆固醇）的胆固醇量的方法和该方法中使用的试剂，本发明可以获得直接测定 通过广泛使用的自动分析仪的LDL-胆固醇的量可以通过使用本发明进行，这在现有方法之后是不可能的。

    Method for measuring the amount of constituent contained in specific lipotrotein
    7.
    发明公开
    Method for measuring the amount of constituent contained in specific lipotrotein 失效
    用于测量包含在特定脂蛋白的组分的量的方法

    公开(公告)号：EP0754948A1

    公开(公告)日：1997-01-22

    申请号：EP96111431.1

    申请日：1996-07-16

    申请人： WAKO PURE CHEMICAL INDUSTRIES, LTD

    发明人： Miki, Yutaka ,  Hanada, Toshiro ,  Tanaka, Kiyoko

    IPC分类号： G01N33/68 ,  G01N33/92 ,  G01N33/53 ,  G01N33/487 ,  G01N21/59

    CPC分类号： G01N33/92 ,  G01N21/59 ,  G01N2800/044 ,  Y10S435/805 ,  Y10S435/962 ,  Y10S436/805 ,  Y10S436/81 ,  Y10S436/811 ,  Y10S436/824 ,  Y10S436/825

    摘要： There is provided a method for measuring the amount of objective constituent for the measurement contained in the specific lipoprotein in a living sample such as serum and plasma, specifically for measuring the amount of cholesterol contained in high density lipoprotein, which can be applicable to clinical tests.
    The present invention also provides a kit for measuring the amount of the objective constituent contained in the specific lipoprotein, which comprises (i) a reagent composition containing an antibody reactive to lipoprotein(s) other than the specific lipoprotein, and a buffering agent; and (ii) a reagent composition containing a reagent for measuring the amount of the objective constituent contained in a lipoprotein and a buffering agent.

    摘要翻译： 提供了一种方法，用于测量目标组分的量对包含在生物样品中的特定脂蛋白的测定：如血清和血浆，特别是用于测量包含在高密度脂蛋白胆固醇的量，其可以是适用于临床试验 ， 因此，本发明提供了用于测量包含在所述特定脂蛋白的目标组分，包括（i）的试剂组合物含有抗体反应的脂蛋白（S）比所述特定脂蛋白和缓冲剂的其它的量的试剂盒; 和（ii）的试剂组合物含有试剂，用于测量包含在脂蛋白和缓冲剂的目标组分的量。