公开(公告)号：EP3201344A1

公开(公告)日：2017-08-09

申请号：EP15780795.9

申请日：2015-09-30

申请人： Zuvasyntha Limited

发明人： GRADLEY, Michelle ,  PUDNEY, Alex ,  HELDT, Dana

IPC分类号： C12P7/18 ,  C12P7/24 ,  C12N9/88

CPC分类号： C12P7/24 ,  C07K2319/00 ,  C12N9/0008 ,  C12N9/88 ,  C12N15/52 ,  C12P7/18 ,  C12Y102/01003 ,  C12Y401/01001 ,  C12Y401/02004 ,  Y02P20/582

摘要： Non-naturally occurring microbial organisms and related methods, processes and materials are for microbial organisms that include a genetic modification which enhances production of 3-hydroxybutanal or a downstream product of 3-hydroxybutanal such as 1,3-butanediol from endogenous central metabolic intermediates such as acetyl CoA or pyruvate which are converted to acetaldehyde. Two molecules of acetaldehyde are condensed to form the 3-hydroxybutanal using an aldolase capable of accepting acetaldehyde as both the acceptor and donor in an aldol condensation. The aldolase may be a deoxyribose phosphate aldolase type enzyme, and is typically introduced into the organisms. Energetically favorable pathways produce 3-hydroxybutanal or downstream products thereof.

摘要翻译： 本发明提供非天然存在的微生物有机体和相关方法，工艺和材料，其中微生物有机体包括遗传修饰，其增强3-羟基丁醛或3-羟基丁醛如1,3-丁二醇的下游产物从内源中央代谢产物 中间体例如乙酰CoA或丙酮酸转化为乙醛，由此使用能够接受乙醛作为醛醇缩合中的受体和供体的醛缩酶使两分子乙醛缩合形成所述3-羟基丁醛。 醛缩酶可以是脱氧核糖磷酸醛缩酶型酶，并且通常被引入到生物体中。 本发明为生产3-羟基丁醛或其下游产物提供了有利的有利途径。