摘要:
A method is provided for the production of organogenic tissue culture and regeneration of whole plants of Glycine species, preferably Glycine max , involving the use of an organogenic culture medium comprising high cytokinin, preferably at least about 10µM BAP, and also preferably comprising at least about 3 times normal concentration of MS micronutrients. The organogenic culture medium is useful for the production of plants embodying desirable characteristics induced by somaclonal variation.
摘要:
Diploid Pelargonium peltatum plants containing a factor resulting in male sterility and/or in their petals at least one of the anthocyanidins pelargonidin and paeonidin, and which may be propagated by seed, as well as the introduction of said characteristics into Pelargonium peltatum using plant breeding and tissue culture techniques.
摘要:
Le procédé décrit permet d'augmenter la vitesse de croissance des plantes. Les plantes sont traitées avec un ou plusieurs acides qui sont des produits de condensation de l'acide glycolique et/ou L-lactique. Ces acides augmentent églement la concentration de chlorophylle, augmentent la vitesse de formation de nouvelles plantes lorsque les plantes se développent par culture des tissus, diminuent la quantité des substances nutritives ajoutées requises à la croissance des plantes, et protègent les plantes contre les effets toxiques des sels. Certains des acides sont utiles pour augmenter la vitesse de formation des racines de la plante.
摘要:
A process for inducing desiccation tolerance in an in vitro formed plant embryo to enable production of viable artificial seeds is disclosed. The process comprises culturing in vitro plant embryos, stimulating the embryos to proceed through globular and a heart shaped stage into an elongate-torpedo shaped stage and early cotyledon stage, inducing the embryos as early as the torpedo-shaped stage with a source of abscisic acid at an effective concentration of abscisic acid and for a sufficient period of time to cause expression of desiccation tolerance which includes change in cellular metabolism, electron transport processes and oxidation-reduction reactions in the embryos and drying the induced embryos to provide stable viable embryos.
摘要:
Disclosed is a method of culturing a protoplast in a liquid medium. According to the method of the present invention, the protoplast is cultured in a liquid medium layer of about 100 to about 400µm thickness.
摘要:
A method for breaking genetic linkage useful for separating desirable traits from undesirable traits in hybrid plants comprising:
a) culturing a callus from a explant of a hybrid plant or from a somatic cell hybrid on a basal medium containing a somatic recombination inducing amount of growth regulator substances and regenerating shoots from said callus; b) rooting said regenerated shoots on a rooting medium to form regenerated plantlets; c) growing said plantlets into mature plants; and d) recovering those plants displaying a recombinant phenotype.
摘要:
The invention is directed to a process for regenerating plants from corn tissue. The process comprises the steps of in vitro conditioning of the corn tissue, culturing the corn tissue on a callus induction medium to induce callus, and regenerating plants from the callus. The callus may optionally be maintained prior to regeneration and the plants may optionally be cultured on an establishment medium prior to transfer to the soil.
摘要:
Somatic hybrid plants of the genus Lycopersicon are produced by fusion of protoplasts of two species one of which cannot be regenerated from protoplast. This procedure enables the introduction of certain desirable traits of wild-type members of the genus into cultivated tomato species which do not regenerate from protoplast.