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公开(公告)号：EP0194809B1
公开(公告)日：1991-03-13
申请号：EP86301586.3
申请日：1986-03-06
发明人： Ahlquist, Paul G. , French, Roy C.
CPC分类号： C12N15/8203 , C12N15/85 , C12N2820/00 , C12N2820/60
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公开(公告)号：EP0318143A3
公开(公告)日：1989-06-07
申请号：EP88309438.5
申请日：1988-10-10
CPC分类号： C07K14/325
摘要： The present invention relates to a novel biocontrol protein which is a crystal toxin and an insecticide and which is produced in a strain of bacteria genetically altered upon incorporation of a specific gene inserted as a stable gene construction. In particular, the present invention relates to the insertion of the δ-endotoxin gene from B. thuringiensis var. tenebrionis into a cloning vector containing prokaryotic regulatory and replication systems and the utilization of this vector to transform bacterial strains into coleopteran pathogens. Limited deletion of the coding region of the gene of this invention still allows preservation of the insecticidal activity in the protein expressed. In addition, this invention provides genetically altered bacterial strains that are insect toxic and that can be used as a novel method of biological control of agricultural insect pests.
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公开(公告)号：EP0208418A3
公开(公告)日：1988-12-21
申请号：EP86304370
申请日：1986-06-09
发明人： Larkins, Brian , Cuellar, Richard
CPC分类号： C12N15/8251 , C07K14/425 , C12N15/8253 , C12N15/8254
摘要： Modified seed storage proteins of maize (zeins) containing lysine and genetically modified structural genes encoding these proteins. The structural genes encoding the 19kd and 22kd zein proteins have been genetically modified so that the zeins are modified to contain lysine. These modifications are made without affecting the ability of the modified zeins to function correctly as a storage protein in maize endosperm. Various genetic constructs and vectors comprising these modified zein genes are provided.
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公开(公告)号：EP0255311A3
公开(公告)日：1988-08-31
申请号：EP87306597
申请日：1987-07-27
CPC分类号： A01N63/00 , C07K14/325 , C12N15/78 , C12N15/90
摘要： ) Methods of biological control of agricultural insect pests are disclosed. These methods utilize biological control agents which are genetically altered strains of root-colonizing strains of members of the species P. cepacia. These strains are genetically altered by introduction of genes encoding insect toxic crystal proteins, and are thereby rendered insect toxic. Genetically altered insect toxic bacterial strains are also provided.
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公开(公告)号：EP0222526A3
公开(公告)日：1988-03-30
申请号：EP86308117
申请日：1986-10-20
发明人： Clarke, Adrienne E. , Mau, Shaio-Lim , Anderson, Marilyn , Cornish, Edwina , Tregear, Geoffrey W. , Crawford, Robert J. , Niall, Hugh D.
IPC分类号： C12N15/00
CPC分类号： C12N15/8287 , C07K14/415 , C07K2319/00 , C07K2319/02
摘要： DNA sequences of an S-gene which encodes S-protein and controls the self-incompatibility reaction in self-incompatible plants. The DNA sequence encoding an S-protein and its attendant full-length signal sequence are provided. A method for the identification and isolation of cDNA and genomic DNA coding sequences of the S-gene are described.
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公开(公告)号：EP0223417A1
公开(公告)日：1987-05-27
申请号：EP86308064.4
申请日：1986-10-17
CPC分类号： C12N15/8205
摘要： Sub-Ti plasmids based on T L -DNA from an octopine-type Ti plasmid are disclosed. Selectable markers funtional in eukaryotes are also exemplified, as are vectors and methods useful in efforts to transform plants.
摘要翻译： 公开了基于章鱼型Ti质粒的TL-DNA的Sub-Ti质粒。 还可以列举在真核生物中有功能的可选择的标记物，以及用于转化植物的载体和方法。
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公开(公告)号：EP0211661A1
公开(公告)日：1987-02-25
申请号：EP86306105.7
申请日：1986-08-07
发明人： Appelbaum, Edward R.
CPC分类号： C07K14/325 , C12N15/52 , C12N15/743
摘要： The promoter of the nitH gene of the fast-growing Rhizobium japonicum strain USDA 191, has been cloned. Over 4.2 kilobase pairs (kbp) of DNA were sequenced. Sequences encoding nitH and the 5'-end of nitD were identified, as were sequences involved in promoting operon transcription and a nifH ribosome binding site. Use of the nitH promoter to drive transcription in Rhizobium of heterologous structural genes is taught. Useful sequences and plasmids are also disclosed.
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公开(公告)号：EP0209370A2
公开(公告)日：1987-01-21
申请号：EP86305457.3
申请日：1986-07-16
CPC分类号： C07K14/325 , C12N15/743
摘要： Complete and partial Bacillus thuringiensis var. kurstaki HD-73 crystal protein protoxin genes were cloned into pRK290. E. coli cells harboring these recombinant plasmids produced protein that was toxic to Manduca sexta (tobacco hornworm) larvae. These plasmids were unstable in and inhibitory of the growth of Agrobacterium and Rhizobium cells; the presence of a complete protoxin gene being far more destabilizing and inhibitory than the presence of a partial protoxin gene. Rhizobium cells containing a partial protoxin gene formed root nodules in legume roots which were toxic to M. sexta larvae. Plasmids carrying complete protoxin genes were totally incompatable with root nodule formation; bacteroids in nodules formed by inoculation with stains containing the complete gene did not contain the gene-carrying plasmid and nodules were not toxic to insect larvae. The invention provides methods to produce insecticidal protein by constructing partial protoxin genes followed by expression of the genes in bacteroids and Rhizobiaceae cells. Useful plasmids and cells are also provided.
摘要翻译： 完整和部分苏云金芽孢杆菌变种 将kurstaki HD-73晶体蛋白原毒素基因克隆到pRK290中。 携带这些重组质粒的大肠杆菌细胞产生对Manduca sexta（烟草蛔虫）幼虫有毒的蛋白质。 这些质粒对农杆菌和根瘤细胞的生长不稳定和抑制; 完整的原毒素基因的存在比部分原毒素基因的存在远远更不稳定和抑制。 含有部分原毒素基因的根瘤细胞在豆科植物根中形成对M. sexta幼虫有毒的根瘤。 携带完整原毒素基因的质粒与根结节形成完全不相符; 通过接种含有完整基因的染色剂形成的结节中的菌体不含有携带基因的质粒，结节对昆虫幼虫无毒性。 本发明提供了通过构建部分原毒素基因，然后在细菌和根瘤菌细胞中表达基因来产生杀虫蛋白的方法。 还提供了有用的质粒和细胞。
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公开(公告)号：EP0208418A2
公开(公告)日：1987-01-14
申请号：EP86304370.9
申请日：1986-06-09
发明人： Larkins, Brian , Cuellar, Richard
CPC分类号： C12N15/8251 , C07K14/425 , C12N15/8253 , C12N15/8254
摘要： Modified seed storage proteins of maize (zeins) containing lysine and genetically modified structural genes encoding these proteins. The structural genes encoding the 19kd and 22kd zein proteins have been genetically modified so that the zeins are modified to contain lysine. These modifications are made without affecting the ability of the modified zeins to function correctly as a storage protein in maize endosperm. Various genetic constructs and vectors comprising these modified zein genes are provided.
摘要翻译： 含有赖氨酸的玉米（玉米醇溶蛋白）和编码这些蛋白质的遗传修饰的结构基因的改良种子储存蛋白。 编码19kd和22kd玉米醇溶蛋白的结构基因已被遗传修饰，因此玉米醇溶蛋白被修饰成含有赖氨酸。 进行这些修饰而不影响修饰的玉米蛋白作为玉米胚乳中的储存蛋白质正常发挥功能。 提供了包含这些修饰的玉米蛋白基因的各种遗传构建体和载体。
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公开(公告)号：EP0194809A1
公开(公告)日：1986-09-17
申请号：EP86301586.3
申请日：1986-03-06
发明人： Ahlquist, Paul G. , French, Roy C.
CPC分类号： C12N15/8203 , C12N15/85 , C12N2820/00 , C12N2820/60
摘要： An RNA molecule for transforming a host cell comprises a cis-acting replication element derived from an RNA virus and further comprises an exogenous RNA segment. The cis-acting replication element may, for example, be derived from a multipartite plant virus, such as tobacco mosaic virus, alfalfa mosaic virus or brome mosaic virus. The exogenous RNA segment may have a regulatory, structural or catalytic property or it may code for a protein or peptide.
摘要翻译： 用于转化宿主细胞的RNA分子包含衍生自RNA病毒的顺式作用复制元件，并且还包含外源性RNA片段。 顺式作用复制元件例如可以衍生自多部分植物病毒，例如烟草花叶病毒，苜蓿花叶病毒或罗马花叶病毒。 外源RNA片段可能具有调节，结构或催化性质，或者可以编码蛋白质或肽。
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