摘要:
According to the method and apparatus of the invention a fermentation liquor is aseptically sampled by withdrawing, a recycle stream of fermentation medium from the fermentation vessel (1), passing the recycle stream through a closed tubular loop (4) back into the fermentation vessel, the loop having at least one tubular section (3) constructed of a porous filter material permeable to the liquor, but not by the culture microorganism or other solids. Liquor which filters. through the porous tubular section is collected as the sample. Internally the tubular section is kept free of deposits clogging the filter by the flow of liquid around the recycle loop.
摘要:
A device for replicating microbial or other cell colonies or aggregates comprises a layer of moisture absorbent material either in the form of a coherent film or comprising individual particles or a combination of film and particles, said layer being secured to a suitable handle forming element by means of which the device may be manipulated.
摘要:
In einem Verfahren und einem Fermenter zur submersen Züchtung von Gewebezellen auf Microcarrier wird das Kulturmedium alternativ über eine im Boden des Kulturgefässes (1) eingebaute Sintermetallplatte (6) begast und abgesaugt. In einer Variante kann die Begasung über einen in einer externen Schlaufe eingebauten Permeator (20) erfolgen. Die Sinterplatte (6) im Fermenter (1) hat den besondern Vorteil, dass Sterilisation und Waschen des Trägermaterials sowie die Züchtung im gleichen Behälter stattfinden können und gleichzeitig als Belüftungsvorrichtung dient.
摘要:
A method of measuring lactic acid or lactate and derivatives thereof in liquids which is extremely versatile and is suitable for use in a number of areas such as the rapid measurement of lactic acid in whole blood, the ratio of lactic acid to pyruvic acid in whole blood, measurement of lactic acid and the study of living lactic acid-producing cells. The acid or lactate is measured by reacting the lactic acid with lactic oxidase to produce pyruvate and H 2 0 2 . The H 2 0 2 is then measured polarographically. The current produced is directly proportional to the lactate level. Preferably, the lactic oxidase is trapped between two semi-permeable membranes. One membrane is placed in contact with an electrolyte at the tip of an electrode and the second membrane contacts the liquid being tested.
摘要翻译:一种在液体中测定乳酸或乳酸盐及其衍生物的方法,其是非常通用的并且适用于多种领域,例如全血中乳酸的快速测定,全血中乳酸与丙酮酸的比例 ,乳酸的测定和活乳酸生产细胞的研究。 通过使乳酸与乳酸氧化酶反应来测定酸或乳酸,以产生丙酮酸和H 2 O 2。 然后用极谱法测量H 2 O 2。 产生的电流与乳酸盐水平成正比。 优选地,乳酸氧化酶被捕获在两个半透膜之间。 将一个膜放置成与电极尖端的电解质接触,并且第二膜接触待测试的液体。
摘要:
A device for replicating microbial or other cell colonies or aggregates comprises a layer of moisture absorbent material either in the form of a coherent film or comprising individual particles or a combination of film and particles, said layer being secured to a suitable handle forming element by means of which the device may be manipulated.
摘要:
A radial flow cell comprises two or more sets of inlet-port-groove-dam (1, 2, 4, 6) devices for improving the efficiency of radial flows and an efficient filtration device consisting of a micropore membrane (9) and a steel screen support (17) of low retention volume. It is useful for aseptic and continuous in-line filtration of heterogeneous mixture containing high concentrations of solids, especially those resulting from fermentation processes.