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公开(公告)号：EP4423231A1
公开(公告)日：2024-09-04
申请号：EP21815545.5
申请日：2021-10-29
申请人： Probiont OY
发明人： POOR, Benedek
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公开(公告)号：EP3330365A1
公开(公告)日：2018-06-06
申请号：EP16201486.4
申请日：2016-11-30
IPC分类号： C12M1/26 , C12N1/02 , A23J1/00 , A23K10/16 , A23K50/10 , A23K50/75 , A23K50/30 , A23K50/80 , A23L3/005 , A23L17/00 , A23L29/00 , A23L33/135 , A23L33/00 , C12M1/00 , A23C1/04 , A21D2/26 , A21D10/00 , A21D13/00
CPC分类号： A23L33/135 , A21D2/267 , A21D2/36 , A21D10/002 , A21D13/00 , A21D13/80 , A23J1/009 , A23K10/16 , A23K50/10 , A23K50/30 , A23K50/75 , A23K50/80 , A23L3/005 , A23L7/109 , A23L17/65 , A23L29/065 , A23L33/40 , C11B1/10 , C11B1/108 , C11B3/16 , C12M33/14 , C12M47/02 , C12N1/02
摘要： The present invention relates to a method of processing microalgae biomass, in particular to a method of processing microalgae biomass comprising the steps of membrane filtration, centrifugation and drying. The centrifugation is preferably performed with moderate centrifugal forces.
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3.发明公开METHODS AND DEVICES FOR BREAKING CELL AGGREGATION AND SEPARATING OR ENRICHING CELLS 审中-公开用于打破细胞聚集和分离或富集细胞的方法和装置
公开(公告)号：EP3242929A1
公开(公告)日：2017-11-15
申请号：EP16735519.7
申请日：2016-01-08
发明人： GUIA, Antonio , TRUONG, Ky
IPC分类号： C12M1/00
CPC分类号： C12M47/04 , C12M23/20 , C12M33/14 , C12M47/02 , C12N13/00 , G01N15/1436 , G01N33/582 , G01N2015/0069 , G01N2015/008 , G01N2015/1006
摘要： In one aspect, the present disclosure provides a method for separating a target component in a fluid sample, which method comprises: a) passing a fluid sample that comprises or is suspected of comprising a target component and cell aggregates through a microfabricated filter so that said target component, if present in said fluid sample, is retained by or passes through said microfabricated filter, and b) prior to and/or concurrently with passing said fluid sample through said microfabricated filter, contacting said fluid sample with an emulsifying agent to reduce or remove said cell aggregates, if present in said fluid sample.
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公开(公告)号：EP3205712A1
公开(公告)日：2017-08-16
申请号：EP15849728.9
申请日：2015-10-05
CPC分类号： G01N33/491 , B01D35/30 , B01D61/147 , B01D61/16 , B01L3/502 , B01L2200/0668 , B01L2300/0681 , C12M33/14 , C12M47/04 , G01N1/4077 , G01N2001/4088 , G01N2800/7028
摘要： A cell-capturing device includes: one or more introduction channels for introducing test liquid containing cells or treatment liquid for treating the cells in the test liquid into the inside thereof; a discharge channel for discharging the test liquid or the treatment liquid to the outside; a filter having a plurality of through-holes formed in a thickness direction, and being disposed in a channel between introduction channel and the discharge channel so that the test liquid or the treatment liquid passes through the through-holes; an introduction region formed between the filter and the introduction channel in a channel between the introduction channel and the discharge channel; a discharge region formed between the filter and the discharge channel; and a housing part accommodating at least a part of the introduction channel, the introduction region, and the discharge region therein, wherein the cell-capturing device further includes a pre-treatment part formed at a position apart from a connection part with the introduction region on at least one of the introduction channels, and formed by a spatial region having a diameter larger than that of the introduction channel.
摘要翻译： 一种细胞捕获装置包括：一个或多个引入通道，用于将含有细胞的测试液体或用于将待测液体中的细胞处理到其内部的处理液; 排出通道，用于将测试液或处理液排出到外部; 过滤器，其具有在厚度方向上形成的多个通孔，并且被布置在引入通道和排出通道之间的通道中，使得测试液体或处理液体穿过通孔; 引入区域，所述引入区域在所述引入通道和所述排出通道之间的通道中形成在所述过滤器和所述引入通道之间; 在过滤器和排放通道之间形成的排放区域; 以及容纳所述导入通道，所述导入区域以及所述排出区域的至少一部分的收纳部，所述细胞捕捉装置还具备在与所述导入区域的连接部分离开的位置形成的前处理部， 在至少一个引入通道上，并且由直径大于引入通道直径的空间区域形成。
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公开(公告)号：EP3194607A1
公开(公告)日：2017-07-26
申请号：EP15842870.6
申请日：2015-09-08
摘要： A method for producing astaxanthin incorporates a method for producing astaxanthin-rich algae cells and a method for extracting astaxanthin therefrom. An initial feedstock comprises healthy algae, water, and nutrients. During a growth phase, carbon dioxide and light from a light source are supplied to the feedstock, thereby amplifying the algae. At least a portion of the nutrients remaining after amplification of the algae are separated from the amplified algae. During a stress phase, carbon dioxide and light are supplied to the amplified algae, thereby promoting production of astaxanthin by the amplified algae. The amplified algae and a cover are placed within an interior of an attrition mill having interior surfaces and media which are substantially non-reactive to astaxanthin and milled to release the astaxanthin from the algae. The cover limits oxidation of the released astaxanthin.
摘要翻译： 本发明的虾青素的制造方法包含富含虾青素的藻类细胞的制造方法以及从中提取虾青素的方法。 初始原料包含健康的藻类，水和营养物质。 在生长阶段，将二氧化碳和来自光源的光提供给原料，从而放大藻类。 从放大的藻类中分离出藻类放大后剩余的至少一部分营养物质。 在胁迫期间，二氧化碳和光被提供给放大的藻类，从而促进放大的藻类产生虾青素。 将放大的藻类和盖子放置在磨碎机的内部，所述磨碎机具有内表面和介质，所述内表面和介质基本上不与虾青素反应并且研磨以从藻类释放虾青素。 盖子限制了释放的虾青素的氧化。
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公开(公告)号：EP3178574A1
公开(公告)日：2017-06-14
申请号：EP15829865.3
申请日：2015-07-30
申请人： Ido, Koji , Ido, Yasumasa
发明人： Ido, Koji , Ido, Yasumasa
CPC分类号： C02F11/04 , B01D61/02 , B01D61/025 , B01D61/14 , B01D61/145 , B01D61/16 , B01D61/58 , B01D2311/04 , B01D2311/08 , B01D2311/25 , B01D2317/025 , B09B3/00 , C02F1/441 , C02F1/444 , C02F3/2853 , C02F11/00 , C02F2303/26 , C12M21/04 , C12M33/14 , C12M45/02 , C12M47/02 , C12P5/023 , Y02E50/343 , B01D2311/2688
摘要： A methane fermentation system which can efficiently generate methane gas is provided.
The methane fermentation system (1) decomposes an organic waste (2) with an anaerobic microorganism to cause methane fermentation so as to generate the methane gas (3). The methane fermentation system (1) includes a wet bead mill (4) which finely pulverizes the organic waste (2). The methane fermentation system (1) also includes a methane fermentation chamber (6) in which the organic waste (2) finely pulverized in the wet bead mill (4) is decomposed with the anaerobic microorganism to cause the methane fermentation so as to generate the methane gas (3) . The organic waste (2) is finely pulverized in the wet bead mill (4) in a state where a moisture content is adjusted such that a solid content is equal to or less than 10%. The organic waste (2) whose moisture content is adjusted in this way is finely pulverized such that a particle diameter at 50% in a cumulative distribution with respect to the volume is equal to or less than 20 µm.摘要翻译： 提供了可以有效地生成甲烷气体的甲烷发酵系统。 甲烷发酵系统（1）将有机废物（2）与厌氧微生物分解，进行甲烷发酵，生成甲烷气（3）。 甲烷发酵系统（1）包括将有机废物（2）细粉碎的湿珠磨机（4）。 甲烷发酵系统（1）还包括甲烷发酵室（6），其中在湿珠磨机（4）中细粉碎的有机废物（2）与厌氧微生物分解以引起甲烷发酵，从而产生 甲烷气（3）。 将有机废弃物（2）在湿珠磨机（4）中以调节固含量等于或小于10％的水分含量的状态下细粉碎。 以这种方式调节其含水量的有机废物（2）被细粉碎，使得相对于体积的累积分布中的50％的粒径等于或小于20μm。
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7.发明公开DISPOSABLE SET FOR CELL CULTURE, CELL CULTURE DEVICE AND CELL PREPARATION METHOD 审中-公开EINWEG-SETFÜRZELLKULTUREN，ZELLKULTURVORRICHTUNG UNDZELLPRÄPARATIONSVERFAHREN
公开(公告)号：EP2589652A4
公开(公告)日：2017-04-05
申请号：EP11800959
申请日：2011-06-30
申请人： KANEKA CORP
CPC分类号： C12M3/06 , C12M23/28 , C12M23/44 , C12M29/00 , C12M29/16 , C12M33/14 , C12M45/00 , C12M47/02 , C12M47/04
摘要： It is an object to achieve a closed system that has a simple structure without resorting to large scale equipment and that sequentially carries out all of the step of separating useful cells, the step of culturing the useful cells separated in the separation step, and the step of washing and concentrating the cells cultured in the culturing step and to enable the preparation of useful cells having high safety and quality, while improving operability. A disposable set for cell culture includes a cell culture container (CC) having a liquid inlet (Lin) and a liquid outlet (Lout), a cell separation kit (A) that is for separating cells used for cell culture and is connected to the liquid inlet (Lin), and a cell collection kit (B) that is for washing and concentrating cultured cells in the cell culture container (CC) and is connected to the liquid outlet (Lout), and a cell culture device uses the disposable set for cell culture and includes flow path on-off valves and pumps provided at appropriate positions of pipelines of the disposable set and a controller controlling them.
摘要翻译： 本发明的目的是实现具有简单结构的封闭系统，而不需要使用大规模设备，并且顺序地执行分离有用细胞的所有步骤，培养在分离步骤中分离的有用细胞的步骤，以及步骤 洗涤和浓缩在培养步骤中培养的细胞，并且能够制备具有高安全性和质量的有用细胞，同时提高可操作性。 用于细胞培养的一次性装置包括具有液体入口（Lin）和液体出口（Lout）的细胞培养容器（CC），用于分离用于细胞培养的细胞的细胞分离试剂盒（A），并连接到 液体入口（Lin）和用于在细胞培养容器（CC）中洗涤和浓缩培养细胞并连接到液体出口（Lout）的细胞收集试剂盒（B），细胞培养装置使用一次性组 用于细胞培养，并且包括在一次性组的管道的适当位置处设置的流路开关阀和泵，以及控制它们的控制器。
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8.发明公开CELL CAPTURING APPARATUS, CELL CAPTURING DEVICE PROVIDED WITH PRE-PROCESSING PART, AND PRE-PROCESSING PART 审中-公开ZELLERFASSUNGSVORRICHTUNG，ZELLERFASSUNGSEINRICHTUNG MIT EINEM VORVERARBEITUNGSTEIL SOWIE VORVERARBEITUNGSTEIL
公开(公告)号：EP3124593A1
公开(公告)日：2017-02-01
申请号：EP14886801.1
申请日：2014-07-17
摘要： By providing a pre-processing unit 20A on a processing liquid channel included in a cell capturing apparatus 100, air bubbles accumulate in an introduction region 24 inside of the pre-processing unit 20A and thus the air bubbles are removed while a processing liquid moves through the introduction region 24 and a discharge region 26, even when the air bubbles are contained in the processing liquid. By providing a foreign matter removal filter 23, at least a part of foreign matters (foreign matters that cannot pass through through-holes 21) contained in the processing liquid can be captured and thus the processing liquid from which the foreign matters are removed can be supplied to a cell capturing device 1 at the subsequent stage. Consequently, the supply of the processing liquid with air bubbles mixed toward the vicinity of a filter 57 of the cell capturing device 1 disposed at the stage subsequent to the pre-processing unit 20A is reduced. This allows the process of cell capturing to be carried out with good repeatability.
摘要翻译： 通过在细胞捕获装置100中包括的处理液体通道上设置预处理单元20A，气泡积聚在预处理单元20A内部的导入区域24中，因此在处理液体移动时除去气泡 引入区域24和放电区域26，即使在处理液体中含有气泡。 通过设置异物去除过滤器23，可以捕获包含在处理液中的至少一部分异物（不能通过通孔21的异物），因此除去异物的处理液可以是 在后续阶段提供给细胞捕获装置1。 因此，配置在预处理单元20A之后的阶段的细胞捕捉装置1的过滤器57附近混合有气泡的处理液的供给减少。 这允许以良好的重复性进行细胞捕获的过程。
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9.发明公开CELL CAPTURE DEVICE, CELL CAPTURE FILTER, CELL CAPTURE APPARATUS, AND METHOD FOR MANUFACTURING CELL CAPTURE DEVICE 审中-公开细胞检测装置，细胞检测过滤单元检测装置及方法产生细胞检测装置
公开(公告)号：EP3124590A1
公开(公告)日：2017-02-01
申请号：EP15770254.9
申请日：2015-03-25
摘要： Cells in a test liquid are captured and observed easily and accurately. A cell capture filter 30 is fixed by a first protrusion part 125 of a lid member 100 and a second protrusion part 225 of a housing member 200 in a cell capture device 1, and a first fitting part 130 and a second fitting part 230 are fitted with each other. This allows the cell capture filter 30 to be fixed between the lid member 100 and the housing member 200 in a state where a filter region of the cell capture filter 30 is surrounded by the looped first protrusion part 125 and the looped second protrusion part 225.
摘要翻译： 在测试液体中的细胞被捕获和观察到的 - 轻松，准确地设定。 细胞捕获过滤器30由盖构件100和在细胞俘获装置1的壳体构件200的第二突出部225，以及第一嵌合部130和第二配合件230的第一突起部125固定装配 witheachother。 这允许细胞捕获过滤器30被固定在盖部件100和其中所述细胞捕获过滤器30的过滤器区域是由环状的第一突出部125和环状的第二突出部225包围的状态下，壳体部件200之间
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公开(公告)号：EP3066189A1
公开(公告)日：2016-09-14
申请号：EP14858814.8
申请日：2014-11-04
CPC分类号： C12Q1/24 , B01D29/055 , B01D29/085 , B01D63/081 , B01D63/087 , B01D65/022 , B01D69/06 , B01D69/12 , B01D2311/2611 , B01D2325/02 , B01D2325/04 , B01D2325/44 , C12M33/14 , C12M47/02 , C12M47/04 , G01N1/31 , G01N1/4077 , G01N2001/4088
摘要： The invention relates to a filtration system for use in a method of determining the presence and/or amount of cells, for example, viable cells, in a liquid sample, and to methods of using and manufacturing such a filtration system. The filtration system includes a cup with an upper portion and a ring portion, where the ring portion is separably coupled to the upper portion.
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