-
公开(公告)号：EP1083235A3
公开(公告)日：2002-07-24
申请号：EP00118757.4
申请日：2000-08-30
摘要： There is provided a reduced coenzyme solution which is stable upon storage for a long period; the said solution contains an active oxygen remover.
-
公开(公告)号：EP1213358A2
公开(公告)日：2002-06-12
申请号：EP01128928.7
申请日：2001-12-05
发明人： Kishimoto, Takahide, Toyo Boseki K.K. , Sogabe, Atsushi, Toyo Boseki K.K. , Hattori, Shizuo, Toyo Boseki K.K. , Oka, Masanori, Toyo Boseki K.K.
CPC分类号： C12N9/0008 , C12Q1/26 , C12Q1/32 , C12Q1/48 , Y10S435/975
摘要： The present invention provides novel glutathione-dependent formaldehyde dehydrogenase that makes possible quantitative measurement of formaldehyde by cycling reaction, and a determination method of formaldehyde and biological components, such as creatinine, creatine, homocysteine and the like, which produces formaldehyde as a reaction intermediate. In addition, the present invention provides a reagent kit for the above-mentioned determination method. The present invention provides a novel determination method of a homocysteine using transferase utilizing homocysteine and other compound as a pair of substrates. Particularly, the present invention provides a determination method of homocysteine which includes bringing betaine-homocysteine methyltransferase and dimethylglycine oxidase into contact with a sample and measuring produced hydrogen peroxide or formaldehyde. Moreover, the present invention provides novel dimethylglycine oxidase stable to thiol compound, which is suitably used for the measurement. The present invention provides a reagent kit used for any of the above-mentioned determination methods of homocysteines.
-
公开(公告)号：EP1185633A2
公开(公告)日：2002-03-13
申请号：EP00940370.0
申请日：2000-06-13
IPC分类号： C12N9/88 , C12N9/02 , C12N15/82 , C12N1/16 , C12N1/21 , C12N5/14 , C12Q1/68 , C12Q1/26 , C12Q1/527
CPC分类号： C12N15/8274 , C12N9/001 , C12N9/88 , C12Q1/26 , C12Q1/32 , C12Q1/527 , G01N2500/00
摘要： The present invention provides plant ENR-A, CBL, UROD, PBGD, and CPPO genes. Also disclosed are the recombinant production of ENR-A, CBL, UROD, PBGD and CPPO enzymes in heterologous hosts, screening chemicals for herbicidal activity using these recombinantly produced enzymes, and the use of thereby identified herbicidal chemicals to suppress the growth of undesired vegetation. Furthermore, the present invention provides methods for the development of herbicide tolerance in plants, plant tissues, plant seeds, and plant cells using ENR-A, CBL, UROD, PBGD, and CPPO genes of the invention.
-
公开(公告)号：EP1136564A2
公开(公告)日：2001-09-26
申请号：EP01105408.7
申请日：2001-03-12
摘要： A two reagent-type kit for assaying GPT by acting GPT on L-alanine and α-ketoglutarate in the presence of pyridoxal phosphate, converting the resulting pyruvate into lactate with L-lactate dehydrogenase (LDH) in the presence of reduced nicotinamide adenine dinucleotide (NADH) and measuring GPT based on a decrement of NADH, the GPT assay kit comprising a first reagent and a second reagent, one of which contains pyridoxal phosphate, L-alanine, α-ketoglutarate, LDH and NADH and the other reagent contains no pyridoxal phosphate but contains L-alanine. The GPT assay kit is stable over a long period of time.
摘要翻译： 在吡哆醛磷酸盐存在下，通过对L-丙氨酸和α-酮戊二酸作用GPT来测定GPT的两种试剂型试剂盒，在还原的烟酰胺腺嘌呤二核苷酸存在下，将所得丙酮酸转化成乳酸L-乳酸脱氢酶（LDH） NADH），并且基于NADH的减少测量GPT，GPT测定试剂盒包含第一试剂和第二试剂，其中之一含有磷酸吡哆醛，L-丙氨酸，α-酮戊二酸，LDH和NADH，另一种试剂不含吡哆醛 磷酸盐，但含有L-丙氨酸。 GPT测定试剂盒在长时间内是稳定的。
-
95.发明公开
公开(公告)号：EP1130111A2
公开(公告)日：2001-09-05
申请号：EP01301670.4
申请日：2001-02-23
申请人： LIFESCAN, INC.
发明人： Ouyang, Tianmei , Yu, Yeung Siu
CPC分类号： C12Q1/32 , C12Q1/26 , C12Q1/54 , G01N33/525 , G01N33/64 , Y10S435/962 , Y10S435/97 , Y10T436/173076
摘要： A reagent is suitable for measuring the concentration of an analyte in a hemoglobin-containing biological fluid, such as whole blood. The reagent comprises a flavin-dependent enzyme that has specificity for the analyte, a flavin cofactor if, and only if, a flavin is not bound to the enzyme, a tetrazolium dye precursor, an electron transfer agent, and a nitrite salt. The reagent causes dye formation that is a measure of the analyte concentration. The nitrite salt suppresses interfering dye formation caused non-enzymatically by the hemoglobin. Preferably, the reagent is used in a dry strip for measuring glucose in whole blood.
摘要翻译： 试剂适用于测量含血红蛋白的生物液体如全血中的分析物的浓度。 试剂包含对分析物具有特异性的黄素依赖性酶，如果并且仅当黄素不与酶结合时，黄素辅因子，四唑染料前体，电子转移剂和亚硝酸盐。 试剂导致染料形成，这是测量分析物浓度。 亚硝酸盐抑制由血红蛋白非酶促引起的干扰染料形成。 优选地，试剂用于测量全血葡萄糖的干燥条中。
-
公开(公告)号：EP1108211A1
公开(公告)日：2001-06-20
申请号：EP99944499.5
申请日：1999-08-24
申请人： UNIVERSITEIT GENT
发明人： REMON, Jean, Paul , ADRIAENS, Els
CPC分类号： C12Q1/32 , C12Q1/42 , G01N33/48 , G01N33/92 , G01N2333/43504
摘要： An in vivo and an in vitro toxicity test method is described using an organism or part of an organism from the class Gastropoda. The method is particularly useful for toxicity testing of chemical substances intended for application to human or animal mucosa, such as the intestinal, vaginal, buccal, nasal or respiratory mucosa. The test method may be used both as a screening test and also as a quantitative toxicity test suitable for ranking the toxicity of chemical substances.
-
公开(公告)号：EP1071810A1
公开(公告)日：2001-01-31
申请号：EP99915363.8
申请日：1999-04-16
CPC分类号： C12Q1/527 , C12Q1/32 , C12Q1/48 , G01N2333/91295 , G01N2500/20
摘要： An assay to screen potential inhibitors of the C4 acid cycle in plants. The assay involves testing inhibition of C4 enzymes by incubating a mixture that includes pyruvate orthophosphate dikinase, phosphoenolpyruvate carboxylase and malate dehydrogenase and their substrates with a potential inhibitor. Detection of inhibition depends on comparing the resulting levels of NADH or NAD+ in the test mixture to the levels of NADH or NAD+ in a control.
-
公开(公告)号：EP1032709A1
公开(公告)日：2000-09-06
申请号：EP98960428.5
申请日：1998-11-24
申请人： Schneider, David R.
发明人： Schneider, David R.
CPC分类号： B01L3/502 , A61B10/0051 , B01L2200/16 , B01L2400/0633 , B01L2400/0672 , B01L2400/0683 , C12Q1/32
摘要： A method and apparatus for the preservation of a saliva sample for use in subsequent quantitative chemical assays. The method involves collecting a saliva sample at a location, directly into a specimen cup. The specimen cup contains a predetermined volume of aqueous solution of pH buffered saline and enzymatic inhibitor and is optionally adapted with a constituent compound specific, qualitative test unit.
-
公开(公告)号：EP0607064B1
公开(公告)日：2000-04-05
申请号：EP94400026.4
申请日：1994-01-05
发明人： Kawakami, Yukari , Fujita, Tsuyoshi
-
100.发明公开
公开(公告)号：EP0866134A3
公开(公告)日：1999-12-22
申请号：EP98103718.7
申请日：1998-03-03
申请人： Hitachi, Ltd.
发明人： Kondo, Shiho , Imai, Kyoko
摘要： A calibrator comprising at least three kinds of enzymes having enzyme activity at least two times the upper reference limit of a reference interval for respective enzyme analysis items is disclosed, and calibration curves for respective of the enzyme analysis items are corrected with the calibrator. The calibrator can be used as a control sample.
According to the above procedure, apparatus differences of measured values can be reduced when an enzyme activity of a testing sample is measured.
-
-
-
-
-
-
-
-
-
搜索结果
856 条记录 (耗时 0.014 秒)
