Abstract:
A solid phase extraction (SPE) cartridge for use in a fluorimetric assay comprises an elongate container (10) of a material permeable to fluorescent radiation, having an open inlet end and an open outlet end. For assay of a compound of interest, a solid adsorbent stationary phase (11) is provided in the container to adsorb the target compound from a mobile phase. To reduce background fluorescence that may interfere with the assay, at least one region (12,13) that is is at leastpartially impermeable to fluorescent radiation, for example by absorbence, scattering or refracting, is provided in or on the container wall, suitably on the outlet side and/or the inlet side of the anticipated location of a band or layer (14) of a compound of interest adsorbed in the stationary phase during an SPE procedure.
Abstract:
The invention relates to an optoelectronic device for measuring the water content in a plant element (10) and to an apparatus designed to evaluate and monitor in real time the state of hydration of the plant covers (10). It enables measurements to be taken repeatedly at certain time intervals on the same material without damaging or destroying it. The device comprises an optoelectronic probe (1) connected to a measurement module (2), the probe (1) comprising: i) a first light source (3) emitting at a wavelength corresponding to a strong water absorption band; ii) optionally, a second light source (4) emitting at a wavelength close to the first source (3) and weakly absorbed by water; and iii) a photo receiver (5) having a spectral response that corresponds to the emission bands of the first light source (3) or of the first (3) and second (4) light sources, the device including means (16) or modulating the average optical power emitted by the light source or sources (3) at a defined frequency, and means (8) for synchronously detecting the light received by the photoreceiver (5). The probe (1) can be carried on a movable member having two jaws (21, 22) between which the specimen (10) is engaged without being in compressive contact therewith.
Abstract:
Described and claimed is an interchangeable tip-open cell fluorometer comprising a housing and a fluorometric probe tip interchangeably connected to the housing, the probe tip including a probe tip housing defining an open cell and enclosing a probe optical arrangement, the probe optical arrangement including an excitation source and a fluorescence detector wherein the excitation source is aimed directly into the fluorescence detector such that a sample can be fluorometrically detected. Also claimed is a method of using this interchangeable tip-open cell fluorometer for detecting fluorescent signals emitted by one or more fluorophores from samples from a natural or industrial water system. The fluorometer, when coupled with a controller, is capable of monitoring and optionally controlling an industrial process or system.
Abstract:
A miniature readhead for a photometric diagnostic instrument includes a housing of hand-held form factor, configured for receiving reagent sample media therein. The sample media has a plurality of test areas configured to react with, and change color, according to an amount of an analyte in a sample. The holder is sized and shaped for forming an indexed fit with the sample media and includes an array of light sources coupled to the housing, each of the light sources configured to emit light onto a respective one of the test areas. An array of chambers respectively containing an array of light detectors, is configured to enable each of the light detectors to receive diffuse, non-specular reflections of the light from the test areas, while substantially preventing the light detectors from receiving specular reflections of the light.
Abstract:
Described and claimed is an interchangeable tip-open cell fluorometer comprising a housing and a fluorometric probe tip interchangeably connected to the housing, the probe tip including a probe tip housing defining an open cell and enclosing a probe optical arrangement, the probe optical arrangement including an excitation source and a fluorescence detector wherein the excitation source is aimed directly into the fluorescence detector such that a sample can be fluorometrically detected. Also claimed is a method of using this interchangeable tip-open cell fluorometer for detecting fluorescent signals emitted by one or more fluorophores from samples from a natural or industrial water system. The fluorometer, when coupled with a controller, is capable of monitoring and optionally controlling an industrial process or system.
Abstract:
The optical head 41 includes first and second members 51, 61 and a tubular member 71. The first member 51 has first to fifth hole portions 52-56 continuously formed so as to penetrate the first member 51. The second member 61 has sixth to eighth hole portions 62-64 continuously formed so as to penetrate the second member 61. This second member 61 is housed in the fifth hole portion 56 and fixed to the first member 51. The tubular member 71 has a first tube portion 72 and a second tube portion 73. The tubular member 71 is housed in the fourth hole portion 55. The lens 27 is sandwiched and fixed between the first tube portion 72 and a step portion formed at a border portion between the second hole portion 53 and the third hole portion 54. The beam shaping member 25 is sandwiched and fixed between the second member 61 and a step portion formed at a border portion between the fourth hole portion 55 and the fifth hole portion 56.
Abstract:
A multiplexed, absorbance-based electrophoresis system for analyzing multiple samples simultaneously without use of a mask or slit comprising a light source, a planar array of capillary tubes and a detector positioned off-axis with the light source and positioned on-axis with and parallel to the planar array of capillary tubes. Other embodiments include vacuum use attached to the capillary tubes to increase the speed of detection.
Abstract:
The invention relates to an optical device for suppressing the stray light from a sample that is illuminated and detected by an objective. A blocking or reflecting-out means is provided in one part of the beam path in order to create an area of shade for the stray light in the image plane.