公开(公告)号：EP1625211A4

公开(公告)日：2010-03-10

申请号：EP04785902

申请日：2004-05-10

申请人： GENENCOR INT

发明人： DODGE TIMOTHY C ,  VALLE FERNANDO ,  KUMAR MANOJ ,  RASHID M HARUNUR

IPC分类号： C12Q1/00 ,  C07K14/24 ,  C12N20060101 ,  C12N1/21 ,  C12N9/10 ,  C12P7/58 ,  C12P7/60 ,  C12P17/04

CPC分类号： C12P7/58 ,  C07K14/24 ,  C12P7/60

公开(公告)号：EP2024506A1

公开(公告)日：2009-02-18

申请号：EP08742796.9

申请日：2008-04-11

申请人： Teva Gyógyszergyár Zártköruen Muködo Részvénytársaság

发明人： TOTH, Laszlo ,  ERDEI, Janos ,  SZIKSZAI, Boglarka ,  BALOGH, Gabor ,  GULYAS, Eva

IPC分类号： C12P17/04 ,  C07D307/88

CPC分类号： C12P17/04 ,  C07D307/88 ,  Y10T436/142222

摘要： The present invention relates to methods for reducing impurities of mycophenolic acid during fermentation by controlling the level of carbon source during fermentation of mycophenolic acid and for the isolation and use as a standard marker of the impurity homo-mycophenolic acid.

公开(公告)号：EP1543135B1

公开(公告)日：2008-12-17

申请号：EP03750589.8

申请日：2003-09-22

申请人： DSM IP Assets B.V.

发明人： HOSHINO, Tatsuo ,  SHINJOH, Masako

IPC分类号： C12P17/04 ,  C12P7/58

CPC分类号： C12P7/58 ,  C12P17/04

摘要： The present invention provides the use of Enzyme B of G. oxydans DSM 4025, as disclosed in EP 0 832 974 A2, in a process for producing L-ascorbic acid from L-gulose, L- galactose, L-idose or L-talose, or from L-gulono-1,4-lactone (and its acid form, L-gulonic acid) and from L-galactono-1,4-lactone (and its acid form, L-galactonic acid).

公开(公告)号：EP1473291B1

公开(公告)日：2008-08-13

申请号：EP03737461.8

申请日：2003-02-03

申请人： MITSUBISHI RAYON CO., LTD.

发明人： KANEKO, Makoto c/o Yokohama Research Lab. ,  NINOMIYA, Yasuhiro c/o Yokohama Research Lab. ,  NAKAMURA, Tetsuji c/o Yokohama Research Lab. ,  SATOU, Eiji c/o Yokohama Research Lab.

IPC分类号： C07D307/33 ,  C12P17/04 ,  C07D307/32 ,  C07D309/30 ,  C07D315/00 ,  C12P13/02

CPC分类号： C07D307/33 ,  C07D309/30 ,  C07D315/00 ,  C12P13/02 ,  C12P17/04 ,  C12P17/06

摘要： A process for producing a lactone which comprises reacting an amide compound represented by the general formula (I): (I) (wherein X represents halogeno; R, R', and R1 to R6 each independently represents hydrogen or any desired substituent; and n is an integer of 0 to 2) with an aqueous medium.

公开(公告)号：EP1859033A1

公开(公告)日：2007-11-28

申请号：EP06706839.5

申请日：2006-02-10

申请人： DSM IP Assets B.V.

发明人： CHEVREUX, Bastien ,  MAYER, Anne, Françoise ,  MEURY, Anja ,  MOUNCEY, Nigel, John ,  SHINJOH, Masako

IPC分类号： C12N9/04 ,  C12P17/04

CPC分类号： C12P17/04 ,  C12N9/0006

摘要： The present invention relates to newly identified genes that encode proteins that are involved in the synthesis of L-ascorbic acid (hereinafter also referred to as Vitamin C). The invention also features polynucleotides comprising the full-length polynucleotide sequences of the novel genes and fragments thereof, the novel polypeptides encoded by the polynucleotides and fragments thereof, as well as their functional equivalents. The present invention also relates to the use of said polynucleotides and polypeptides as biotechnological tools in the production of Vitamin C from microorganisms, whereby a modification of said polynucleotides and/or encoded polypeptides has a direct or indirect impact on yield, production, and/or efficiency of production of the fermentation product in said microorganism. Also included are methods/processes of using the polynucleotides and modified polynucleotide sequences to transform host microorganisms. The invention also relates to genetically engineered microorganisms and their use for the direct production of Vitamin C.

公开(公告)号：EP1853715A1

公开(公告)日：2007-11-14

申请号：EP06706856.9

申请日：2006-02-10

申请人： DSM IP Assets B.V.

发明人： CHEVREUX, Bastien ,  MAYER, Anne Françoise ,  MEURY, Anja ,  MOUNCEY, Nigel John ,  SHINJOH, Masako

IPC分类号： C12P17/04 ,  C12P7/60

CPC分类号： C12P17/04 ,  C07K14/195

摘要： The present invention relates to newly identified genes that encode proteins that are involved in the synthesis of L-ascorbic acid (hereinafter also referred to as Vitamin C). The invention also features polynucleotides comprising the full-length polynucleotide sequences of the novel genes and fragments thereof, the novel polypeptides encoded by the polynucleotides and fragments thereof, as well as their functional equivalents. The present invention also relates to the use of said polynucleotides and polypeptides as biotechnological tools in the production of Vitamin C from microorganisms, whereby a modification of said polynucleotides and/or encoded polypeptides has a direct or indirect impact on yield, production, and/or efficiency of production of the fermentation product in said microorganism. Also included are methods/processes of using the polynucleotides and modified polynucleotide sequences to transform host microorganisms. The invention also relates to genetically engineered microorganisms and their use for the direct production of Vitamin C.

摘要翻译： 本发明涉及编码参与L-抗坏血酸合成的蛋白质（以下也称为维生素C）的新鉴定的基因。 本发明的特征还在于包含新基因及其片段的全长多核苷酸序列的多核苷酸，由该多核苷酸及其片段编码的新多肽以及它们的功能等价物。 本发明还涉及所述多核苷酸和多肽作为生物技术工具在由微生物生产维生素C中的用途，由此修饰所述多核苷酸和/或编码的多肽对产量，产量和/或 所述微生物中发酵产物的生产效率。 还包括使用多核苷酸和修饰的多核苷酸序列转化宿主微生物的方法/过程。 本发明还涉及基因工程微生物及其用于直接生产维生素C的用途。

公开(公告)号：EP1853701A1

公开(公告)日：2007-11-14

申请号：EP06723020.1

申请日：2006-02-10

申请人： DSM IP Assets B.V.

发明人： CHEVREUX, Bastien ,  MAYER, Anne Françoise ,  SHINJOH, Masako

IPC分类号： C12N9/04 ,  C12P17/04

CPC分类号： C12P17/04 ,  C12N9/0006 ,  C12P7/60

摘要： The present invention relates to newly identified genes that encode proteins that are involved in the synthesis of L-ascorbic acid (hereinafter also referred to as Vitamin C). The invention also features polynucleotides comprising the full-length polynucleotide 5 sequences of the novel genes and fragments thereof, the novel polypeptides encoded by the polynucleotides and fragments thereof, as well as their functional equivalents. The present invention also relates to the use of said polynucleotides and polypeptides as biotechnological tools in the production of Vitamin C from microorganisms, whereby a modification of said polynucleotides and/or encoded polypeptides has a direct or indirect 0 impact on yield, production, and/or efficiency of production of the fermentation product in said microorganism. Also included are methods/processes of using the polynucleotides and modified polynucleotide sequences to transform host microorganisms. The invention also relates to genetically engineered microorganisms and their use for the direct production of Vitamin C.

公开(公告)号：EP1846553A1

公开(公告)日：2007-10-24

申请号：EP06706840.3

申请日：2006-02-10

申请人： DSM IP Assets B.V.

发明人： HOSHINO, Tatsuo ,  SHINJOH, Masako ,  TOMIYAMA, Noribumi

IPC分类号： C12N9/04 ,  C12P17/04

CPC分类号： C12P17/04 ,  C12N9/0006

摘要： The present invention relates to newly identified genes that encode proteins that are involved in the synthesis of L-ascorbic acid (hereinafter also referred to as Vitamin C). The invention also features polynucleotides comprising the full-length polynucleotide sequences of the novel genes and fragments thereof, the novel polypeptides encoded by the polynucleotides and fragments thereof, as well as their functional equivalents. The present invention also relates to the use of said polynucleotides and polypeptides as biotechnological tools in the production of Vitamin C from microorganisms, whereby a modification of said polynucleotides and/or encoded polypeptides has a direct or indirect impact on yield, production, and/or efficiency of production of the fermentation product in said microorganism. Also included are methods/processes of using the polynucleotides and modified polynucleotide sequences to transform host microorganisms. The invention also relates to genetically engineered microorganisms and their use for the direct production of Vitamin C.

摘要翻译： 本发明涉及编码参与L-抗坏血酸合成的蛋白质（以下也称为维生素C）的新鉴定的基因。 本发明的特征还在于包含新基因及其片段的全长多核苷酸序列的多核苷酸，由该多核苷酸及其片段编码的新多肽以及它们的功能等价物。 本发明还涉及所述多核苷酸和多肽作为生物技术工具在由微生物生产维生素C中的用途，由此修饰所述多核苷酸和/或编码的多肽对产量，产量和/或 所述微生物中发酵产物的生产效率。 还包括使用多核苷酸和修饰的多核苷酸序列转化宿主微生物的方法/过程。 本发明还涉及基因工程微生物及其用于直接生产维生素C的用途。

公开(公告)号：EP1736550A1

公开(公告)日：2006-12-27

申请号：EP05425452.9

申请日：2005-06-22

申请人： Adorkem Technology SpA

发明人： Cotticelli, Giovanni ,  Salvetti, Raul ,  Bertoni, Chiara

IPC分类号： C12P13/02 ,  C12P17/04 ,  C12P9/00 ,  C07D307/87

CPC分类号： C12P17/14

摘要： A process is described for the preparation of escitalopram and the pharmaceutically acceptable salts thereof starting from 5-cyanophthalide by a process which provides an enantioselective enzymatic deacylation reaction of a complex of the formula

where R represents a C 1 -C 4 alkyl residue or an aryl residue under the action of an esterase from Aspergillus niger.

摘要翻译： 描述了从5-氰基苯酞起始制备依他普仑及其药学上可接受的盐的方法，该方法提供下式配合物的对映选择性酶脱酰反应，其中R代表C 1 -C 4烷基或芳基 残留物在黑曲霉酯酶的作用下。

公开(公告)号：EP1550725A1

公开(公告)日：2005-07-06

申请号：EP03730820.2

申请日：2003-06-05

申请人： KANEKA CORPORATION

发明人： OHISHI, Takahiro, c/o KANEKA CORPORATION ,  NANBA, Hirokazu, c/o KANEKA CORPORATION ,  SUGAWARA, Masanobu, c/o KANEKA CORPORATION ,  IZUMIDA, Masashi, c/o KANEKA CORPORATION ,  HONDA, Tatsuya, c/o KANEKA CORPORATION ,  MORI, Kohei, c/o KANEKA CORPORATION ,  YANAGISAWA, Satohiro, c/o KANEKA CORPORATION ,  NAGASHIMA, Nobuo, c/o KANEKA CORPORATION ,  INOUE, Kenji, c/o KANEKA CORPORATION

IPC分类号： C12P13/12 ,  C12P17/04 ,  C07C323/58 ,  C07C319/02 ,  C07C319/14

CPC分类号： C12P13/12 ,  C07C319/06 ,  C07D233/76 ,  C12P17/04 ,  C12P17/10 ,  C12P41/009 ,  C07C323/58

摘要： The present invention provides a simple industrial process for producing an L- or D-optionally active α-methylcysteine derivative or its salt, which is a useful pharmaceutical intermediate, from readily available, inexpensive raw materials.
In a process for producing an L- or D-optically active α- methylcysteine derivative or its salt, a racemic N-carbamoyl-α- methylcysteine derivative or its salt is D-selectively cyclized with hydantoinase to produce a D-5-methyl-5-thiomethylhydantoin derivative or its salt and an N-carbamoyl-α-methyl-L-cysteine derivative or its salt, which are then subjected to deprotection of the amino group and the sulfur atom, and hydrolysis.

摘要翻译： 本发明提供了一种简单的工业方法，用于从容易获得的廉价原料生产L-或D-任选的活性α-甲基半胱氨酸衍生物或其盐，其是有用的药物中间体。 在制备L-或D-光学活性α-甲基半胱氨酸衍生物或其盐的方法中，外消旋N-氨基甲酰基-α-甲基半胱氨酸衍生物或其盐与乙内酰脲酶D选择性环化以产生D- 5-甲基-5-硫代甲基乙内酰脲衍生物或其盐和N-氨基甲酰基-α-甲基-L-半胱氨酸衍生物或其盐，然后进行氨基和硫原子的脱保护和水解。