公开(公告)号：EP1846551A1

公开(公告)日：2007-10-24

申请号：EP06706854.4

申请日：2006-02-10

申请人： DSM IP Assets B.V.

发明人： CHEVREUX, Bastien ,  MAYER, Anne Françoise ,  MEURY, Anja ,  MOUNCEY, Nigel John ,  SHINJOH, Masako

IPC分类号： C12N9/00 ,  C12P17/04

CPC分类号： C12P17/04 ,  C07K14/195

摘要： The present invention relates to newly identified genes that encode proteins that are involved in the synthesis of L-ascorbic acid (hereinafter also referred to as Vitamin C). The invention also features polynucleotides comprising the full-length polynucleotide sequences of the novel genes and fragments thereof, the novel polypeptides encoded by the polynucleotides and fragments thereof, as well as their functional equivalents. The present invention also relates to the use of said polynucleotides and polypeptides as biotechnological tools in the production of Vitamin C from microorganisms, whereby a modification of said polynucleotides and/or encoded polypeptides has a direct or indirect impact on yield, production, and/or efficiency of production of the fermentation product in said microorganism. Also included are methods/processes of using the polynucleotides and modified polynucleotide sequences to transform host microorganisms. The invention also relates to genetically engineered microorganisms and their use for the direct production of Vitamin C.

摘要翻译： 本发明涉及编码参与L-抗坏血酸合成的蛋白质（以下也称为维生素C）的新鉴定的基因。 本发明的特征还在于包含新基因及其片段的全长多核苷酸序列的多核苷酸，由该多核苷酸及其片段编码的新多肽以及它们的功能等价物。 本发明还涉及所述多核苷酸和多肽作为生物技术工具在由微生物生产维生素C中的用途，由此修饰所述多核苷酸和/或编码的多肽对产量，产量和/或 所述微生物中发酵产物的生产效率。 还包括使用多核苷酸和修饰的多核苷酸序列转化宿主微生物的方法/过程。 本发明还涉及基因工程微生物及其用于直接生产维生素C的用途。

公开(公告)号：EP1846440A1

公开(公告)日：2007-10-24

申请号：EP06706855.1

申请日：2006-02-10

申请人： DSM IP Assets B.V.

发明人： CHEVREUX, Bastien ,  MAYER, Anne Françoise ,  MEURY, Anja ,  MOUNCEY, Nigel John ,  SHINJOH, Masako

IPC分类号： C07K14/195 ,  C12P17/04

CPC分类号： C12P7/60 ,  C07K14/195 ,  C12P17/04

摘要： The present invention relates to a newly identified gene that encodes a protein that is involved in the synthesis of L-ascorbic acid (hereinafter also referred to a Vitamin C). The protein is coenzyme PQQ synthesis protein B. The invention also features polynucleotides comprising the full-length polynucleotide sequences of the novel genes and fragments thereof, the novel polypeptides encoded by the polynucleotides and fragments thereof, as well as their functional equivalents. The present invention also relates to the use of said polynucleotides and polypeptides as biotechnological tools in the production of Vitamin C from microorganisms, whereby a modification of said polynucleotides and/or encoded polypeptides has a direct or indirect impact on yield, production, and/or efficiency of production of the fermentation product in said microorganism. Also included are methods/processes of using the polynucleotides and modified polynucleotide sequences to transform host microorganisms. The invention also relates to genetically engineered microorganisms and their use for the direct production of Vitamin C.

公开(公告)号：EP1846565A1

公开(公告)日：2007-10-24

申请号：EP06706853.6

申请日：2006-02-10

申请人： DSM IP Assets B.V.

发明人： CHEVREUX, Bastien ,  MAYER, Anne Françoise ,  MEURY, Anja ,  MOUNCEY, Nigel John ,  SHINJOH, Masako

IPC分类号： C12P17/04 ,  C12P7/60

CPC分类号： C12P17/04 ,  C07K14/195

摘要： The present invention relates to newly identified genes that encode proteins that are involved in the synthesis of L-ascorbic acid (hereinafter also referred to as Vitamin C). The invention also features polynucleotides comprising the full-length polynucleotide sequences of the novel genes and fragments thereof, the novel polypeptides encoded by the polynucleotides and fragments thereof, as well as their functional equivalents. The present invention also relates to the use of said polynucleotides and polypeptides as biotechnological tools in the production of Vitamin C from microorganisms, whereby a modification of said polynucleotides and/or encoded polypeptides has a direct or indirect impact on yield, production, and/or efficiency of production of the fermentation product in said microorganism. Also included are methods/processes of using the polynucleotides and modified polynucleotide sequences to transform host microorganisms. The invention also relates to genetically engineered microorganisms and their use for the direct production of Vitamin C.

摘要翻译： 本发明涉及编码参与L-抗坏血酸合成的蛋白质（以下也称为维生素C）的新鉴定的基因。 本发明的特征还在于包含新基因及其片段的全长多核苷酸序列的多核苷酸，由该多核苷酸及其片段编码的新多肽以及它们的功能等价物。 本发明还涉及所述多核苷酸和多肽作为生物技术工具在由微生物生产维生素C中的用途，由此修饰所述多核苷酸和/或编码的多肽对产量，产量和/或 所述微生物中发酵产物的生产效率。 还包括使用多核苷酸和修饰的多核苷酸序列转化宿主微生物的方法/过程。 本发明还涉及基因工程微生物及其用于直接生产维生素C的用途。

公开(公告)号：EP1846555A1

公开(公告)日：2007-10-24

申请号：EP06706842.9

申请日：2006-02-10

申请人： DSM IP Assets B.V.

发明人： CHEVREUX, Bastien ,  MAYER, Anne Françoise ,  MEURY, Anja ,  MOUNCEY, Nigel John ,  SHINJOH, Masako

IPC分类号： C12N9/04 ,  C12P17/04

CPC分类号： C12P17/04 ,  C12N9/0006

摘要： The present invention relates to newly identified genes that encode proteins that are involved in the synthesis of L-ascorbic acid (hereinafter also referred to as Vitamin C). The invention also features polynucleotides comprising the full-length polynucleotide sequences of the novel genes and fragments thereof, the novel polypeptides encoded by the polynucleotides and fragments thereof, as well as their functional equivalents. The present invention also relates to the use of said polynucleotides and polypeptides as biotechnological tools in the production of Vitamin C from microorganisms, whereby a modification of said polynucleotides and/or encoded polypeptides has a direct or indirect impact on yield, production, and/or efficiency of production of the fermentation product in said microorganism. Also included are methods/processes of using the polynucleotides and modified polynucleotide sequences to transform host microorganisms. The invention also relates to genetically engineered microorganisms and their use for the direct production of Vitamin C.

公开(公告)号：EP1853715A1

公开(公告)日：2007-11-14

申请号：EP06706856.9

申请日：2006-02-10

申请人： DSM IP Assets B.V.

发明人： CHEVREUX, Bastien ,  MAYER, Anne Françoise ,  MEURY, Anja ,  MOUNCEY, Nigel John ,  SHINJOH, Masako

IPC分类号： C12P17/04 ,  C12P7/60

CPC分类号： C12P17/04 ,  C07K14/195

摘要： The present invention relates to newly identified genes that encode proteins that are involved in the synthesis of L-ascorbic acid (hereinafter also referred to as Vitamin C). The invention also features polynucleotides comprising the full-length polynucleotide sequences of the novel genes and fragments thereof, the novel polypeptides encoded by the polynucleotides and fragments thereof, as well as their functional equivalents. The present invention also relates to the use of said polynucleotides and polypeptides as biotechnological tools in the production of Vitamin C from microorganisms, whereby a modification of said polynucleotides and/or encoded polypeptides has a direct or indirect impact on yield, production, and/or efficiency of production of the fermentation product in said microorganism. Also included are methods/processes of using the polynucleotides and modified polynucleotide sequences to transform host microorganisms. The invention also relates to genetically engineered microorganisms and their use for the direct production of Vitamin C.

摘要翻译： 本发明涉及编码参与L-抗坏血酸合成的蛋白质（以下也称为维生素C）的新鉴定的基因。 本发明的特征还在于包含新基因及其片段的全长多核苷酸序列的多核苷酸，由该多核苷酸及其片段编码的新多肽以及它们的功能等价物。 本发明还涉及所述多核苷酸和多肽作为生物技术工具在由微生物生产维生素C中的用途，由此修饰所述多核苷酸和/或编码的多肽对产量，产量和/或 所述微生物中发酵产物的生产效率。 还包括使用多核苷酸和修饰的多核苷酸序列转化宿主微生物的方法/过程。 本发明还涉及基因工程微生物及其用于直接生产维生素C的用途。

公开(公告)号：EP1853701A1

公开(公告)日：2007-11-14

申请号：EP06723020.1

申请日：2006-02-10

申请人： DSM IP Assets B.V.

发明人： CHEVREUX, Bastien ,  MAYER, Anne Françoise ,  SHINJOH, Masako

IPC分类号： C12N9/04 ,  C12P17/04

CPC分类号： C12P17/04 ,  C12N9/0006 ,  C12P7/60

摘要： The present invention relates to newly identified genes that encode proteins that are involved in the synthesis of L-ascorbic acid (hereinafter also referred to as Vitamin C). The invention also features polynucleotides comprising the full-length polynucleotide 5 sequences of the novel genes and fragments thereof, the novel polypeptides encoded by the polynucleotides and fragments thereof, as well as their functional equivalents. The present invention also relates to the use of said polynucleotides and polypeptides as biotechnological tools in the production of Vitamin C from microorganisms, whereby a modification of said polynucleotides and/or encoded polypeptides has a direct or indirect 0 impact on yield, production, and/or efficiency of production of the fermentation product in said microorganism. Also included are methods/processes of using the polynucleotides and modified polynucleotide sequences to transform host microorganisms. The invention also relates to genetically engineered microorganisms and their use for the direct production of Vitamin C.

公开(公告)号：EP1846554A1

公开(公告)日：2007-10-24

申请号：EP06706841.1

申请日：2006-02-10

申请人： DSM IP Assets B.V.

发明人： BEUZELIN OLLIVIER, Marie-Gabrielle ,  CHEVREUX, Bastien ,  DALLUEGE, Manuela ,  GELDER VAN, Marina ,  GOESE, Markus ,  HAUK, Corina ,  KOEKMAN, Bertus Pieter ,  LEE, Connie ,  MAYER, Anne Françoise ,  MEURY, Anja ,  MOUNCEY, Nigel John ,  SCHIPPER, Dick ,  SHINJOH, Masako ,  TOEPFER, Christine ,  VOLLEBREGT, Adrianus Wilhelmus Hermanus

IPC分类号： C12N9/04

CPC分类号： C12P17/04 ,  C12N9/0008

摘要： The present invention relates to newly identified microorganisms capable of direct production of L-ascorbic acid (hereinafter also referred to as Vitamin C). The invention also relates to polynucleotide sequences comprising genes that encode proteins which are involved in the synthesis of Vitamin C. The invention also features polynucleotides comprising the full length polynucleotide sequences of the novel genes and fragments thereof, the novel polypeptides encoded by the polynucleotides and fragments thereof, as well as their functional equivalents. The present invention also relates to the use of said polynucleotides and polypeptides as biotechnological tools in the production of Vitamin C from microorganisms, whereby a modification of said polynucleotides and/or encoded polypeptides has a direct or indirect impact on yield, production, and/or efficiency of production of the fermentation product in said microorganism. Also included are methods/processes of using the polynucleotides and modified polynucleotide sequences to transform host microorganisms. The invention also relates to genetically engineered microorganisms and their use for the direct production of Vitamin C.