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    MODIFIED ELECTRODE BUFFERS FOR STAIN-FREE PROTEIN DETECTION IN ELECTROPHORESIS
    15.
    发明公开
    MODIFIED ELECTRODE BUFFERS FOR STAIN-FREE PROTEIN DETECTION IN ELECTROPHORESIS 审中-公开
    修饰电极缓冲FOR电泳污点FREE蛋白质检测

    公开(公告)号：EP2882524A1

    公开(公告)日：2015-06-17

    申请号：EP13828567.1

    申请日：2013-08-07

    申请人： Bio-Rad Laboratories, Inc.

    发明人： BELISLE, Christopher

    IPC分类号： B01D57/02 ,  G01N27/447

    CPC分类号： G01N27/44747 ,  G01N27/44713 ,  G01N27/44721 ,  G01N27/44726 ,  G01N27/44739

    摘要： Proteins that are electrophoretically separated in a gel are derivatized to produce fluorescent emissions by incorporating halo-substituted organic compounds into one or both of the electrode buffer solutions at the two ends of the gel. The halo-substituted compounds used are ones that bear an electric charge at the pH of the buffer solutions and gel, and the polarity of the charge on the compounds is such that the compounds migrate from the electrode buffer into the gel under the electrophoretic influence concurrently with the migration of the proteins into the gel. Once the proteins are separated and distributed within the gel and the gel is fully penetrated with the halo-substituted compounds, the gel is irradiated with ultraviolet light to induce a reaction between the halo-substituted compounds and the proteins through the tryptophan residues on the proteins, producing fluorescent reaction products.