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    PROTEIN DETECTION USING MODIFIED CYCLODEXTRINS
    4.
    发明公开
    PROTEIN DETECTION USING MODIFIED CYCLODEXTRINS 审中-公开
    蛋白酶抑制剂MITHILDE MODEMIZERER CYCLODEXTRINEN

    公开(公告)号：EP2986646A1

    公开(公告)日：2016-02-24

    申请号：EP14834424.5

    申请日：2014-08-08

    申请人： Bio-rad Laboratories, Inc.

    发明人： MCKEE, Clayton T. ,  STRONG, William ,  OLECH, Lee ,  BERKELMAN, Thomas ,  BELISLE, Christopher

    IPC分类号： C08B37/16

    CPC分类号： G01N33/5306 ,  G01N27/44747 ,  G01N33/582 ,  G01N33/6803 ,  G01N2400/18

    摘要： A method is provided for detecting a protein using a cyclodextrin covalently linked to at least one label. The cyclodextrin can associate with the protein by sequestering an aromatic amino acid side-chain of the protein in its hydrophobic cavity. After contacting the protein with the cyclodextrin, the label can be detected directly or can undergo a chemical interaction with a reagent to form a detectable product. The label can include an indole moiety, which can react with a halo-substituted organic compound upon exposure to UV light and thereby be rendered fluorescent. Alternatively, the label can include a biotin moiety, which can bind to a binding partner such as avidin, or variants thereof, to form a detectable molecular complex.

    摘要翻译： 提供了一种使用共价连接至少一个标签的环糊精来检测蛋白质的方法。 环糊精可以通过在其疏水腔中螯合蛋白质的芳香族氨基酸侧链与蛋白质结合。 将蛋白质与环糊精接触后，可以直接检测标记物，或者可以与试剂进行化学相互作用以形成可检测的产物。 该标记可以包括吲哚部分，其可以在暴露于UV光时与卤素取代的有机化合物反应，从而变成荧光。 或者，标记可以包括生物素部分，其可结合结合配偶体例如抗生物素蛋白或其变体，以形成可检测的分子复合物。 本方法中可以使用标记的环糊精来检测电泳凝胶或印迹膜上的目标蛋白质。 蛋白质，特别是色氨酸的芳香族氨基酸残基由于环糊精的螯合而保护免受化学修饰，使得这些方法与需要完整蛋白质的下游应用相容。 本文还提供了用于检测蛋白质的组合物，试剂盒和电泳凝胶。

    POLYACRYLAMIDE GELS FOR RAPID CASTING, BLOTTING, AND IMAGING, WITH STORAGE STABILITY
    8.
    发明公开
    POLYACRYLAMIDE GELS FOR RAPID CASTING, BLOTTING, AND IMAGING, WITH STORAGE STABILITY 审中-公开
    聚丙烯酰胺FOR迅速融化，吸收和成像保质期

    公开(公告)号：EP2969141A1

    公开(公告)日：2016-01-20

    申请号：EP14769469.9

    申请日：2014-03-10

    申请人： Bio-Rad Laboratories, Inc.

    发明人： LI, Lei ,  YANG, Xuemei ,  BELISLE, Christopher

    IPC分类号： B01D57/02 ,  G01N27/447

    CPC分类号： G01N27/44747 ,  B01D57/02 ,  B65D85/70 ,  C07K1/26 ,  C08F2/46 ,  C08K3/00 ,  G01N21/6428

    摘要： A hand cast gel system for polyacrylamide gel electrophoresis is provided. In some embodiments, the system comprises: a rigid mold that contains the polyacrylamide gel; and the polyacrylamide gel, comprising acrylamide, triethanolamine, an ampholyte selected from the group consisting of glycine and tricine, and a conjugate ampholyte consisting of an amino acid with a pKa within the range of 8.3 to 9.6. Advantages include the ability to store the gel for extended periods of time (e.g. up to 30 days in bottom-open glass cassettes or even longer with sealed plastic cassettes) prior to use with little or no deterioration, the ability to run the electrophoresis experiment faster than is typically done with typical hand-cast gels, the ability to blot or transfer proteins from the gel to a membrane faster than with typical hand-cast gels, and the ability to image gels without a staining, i.e., in a "stain-free" manner.