公开(公告)号：EP0419858A1

公开(公告)日：1991-04-03

申请号：EP90116258.6

申请日：1990-08-24

申请人： Juridical Foundation The Chemo-Sero-Therapeutic Research Institute

发明人： Maeda, Hiroaki ,  Eda, Yasuyuki ,  Kimachi, Kazuhiko ,  Ono, Yoichi ,  Tokiyoshi, Sachio

IPC分类号： C12N15/13 ,  C07K16/46 ,  C12N5/26

CPC分类号： C07K16/462 ,  A61K39/00 ,  C07K2317/24

摘要： A gene fragment which comprises a DNA sequence coding for an amino acid sequence of a constant region of canine im­munoglobulin lambda chain; a gene fragment which comprises a DNA sequence coding for an amino acid sequence of a constant region of canine immunoglobulin kappa chain; a gene fragment which com­prises a DNA sequence coding for the constant region of canine immunoglobulin gamma chain; a recombinant DNA molecule coding for an amino acid sequence of a mouse-dog chimeric antibody which comprises a gene fragment coding for an amino acid sequence of a variable region of a mouse immunoglobulin and a gene fragment coding for an amino acid sequence of a constant region of a canine immunoglobulin wherein the latter gene fragment; a polypeptide of a mouse-dog chimeric antibody which is expressed from a cell transformed with an expression vector for cells wherein said recombinant DNA molecule cording for an amino acid sequence of the mouse-dog chimeric antibody is incorporated; a dog-mouse heterohybridoma which produces canine immunoglobulin; and a process for preparing a canine im­munoglobulin gene.

摘要翻译： 一种基因片段，其包含编码犬免疫球蛋白λ链的恒定区的氨基酸序列的DNA序列; 包含编码犬免疫球蛋白κ链恒定区氨基酸序列的DNA序列的基因片段; 一种基因片段，其包含编码犬免疫球蛋白γ链恒定区的DNA序列; 编码小鼠 - 狗嵌合抗体的氨基酸序列的重组DNA分子，其包含编码小鼠免疫球蛋白的可变区的氨基酸序列的基因片段和编码恒定区的氨基酸序列的基因片段 的犬免疫球蛋白，其中后者基因片段; 从用细胞表达载体转化的细胞中表达小鼠 - 狗嵌合抗体的多肽，其中并入所述针对小鼠 - 狗嵌合抗体的氨基酸序列的重组DNA分子; 产生犬免疫球蛋白的狗 - 小鼠异源杂交瘤; 以及制备犬免疫球蛋白基因的方法。