公开(公告)号：EP1313862A1

公开(公告)日：2003-05-28

申请号：EP01951447.0

申请日：2001-07-09

申请人： Novozymes A/S

发明人： PEDERSEN, Poul, Erik ,  ROGGEN, Erwin, Ludo

IPC分类号： C12N15/62 ,  C07K14/81 ,  C12N9/50 ,  C11D3/386

CPC分类号： C11D3/386

摘要： The present invention provides a method for producing a protein complex comprising the steps of constructing a fusion polynucleotide sequence in frame, the sequence comprising a first gene encoding a protease, and a second gene encoding a protease inhibitor; introducing the sequence into a host cell; cultivating the host cell, wherein the cell expresses the sequence and produces a non-covalently linked complex of the protease and the inhibitor; and recovering the complex.

公开(公告)号：EP1224468A1

公开(公告)日：2002-07-24

申请号：EP00967617.2

申请日：2000-10-13

申请人： Novozymes A/S

发明人： ROGGEN, Erwin, Ludo ,  ERNST, Steffen

IPC分类号： G01N33/68

CPC分类号： G01N33/6863 ,  G01N2333/535 ,  G01N2333/54 ,  G01N2333/5412

摘要： The invention relates to a method for the assessment of the allergenicity or the toxicity of a test compound and for the screening of at least two compounds simultaneously. The invention also relates to a method of characterising a cell type for the present invention, as well as an assay kit for the high through-put screening of a compound. By contacting a cell culture of at least one cell type of animal origin with the test compound and measuring cytokine responses, the allergenicity of the test compound can be assessed.

公开(公告)号：EP1124946A1

公开(公告)日：2001-08-22

申请号：EP99947259.0

申请日：1999-10-12

申请人： Novozymes A/S

发明人： OLSEN, Arne, Agerlin ,  ROGGEN, Erwin, Ludo ,  ERNST, Steffen

IPC分类号： C12N15/00 ,  C12N9/96 ,  C07K1/06

CPC分类号： C12Y301/01003 ,  A61K38/00 ,  C12N9/20 ,  C12P21/005

摘要： The present invention relates to the field of immunology, in particular allergenicity. More specifically it relates to the reduction of allergenicity by the in vivo N-glycosylation of proteins.