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1.发明公开
公开(公告)号：EP1680503A1
公开(公告)日：2006-07-19
申请号：EP04762825.0
申请日：2004-09-13
申请人： Novozymes A/S
发明人： JENSEN, Ejner, Bech , HÖGENHAUG, Hans-Henrik, Kristensen , HANSEN, Peter, Kamp , PEDERSEN, Poul, Erik , MYGIND, Per, Holse
IPC分类号： C12N5/10 , A23K1/17 , C07K14/47 , C12N15/62 , C12N9/90 , A23K1/00 , C12N15/82 , A01K67/027 , C07K1/107
CPC分类号： C12N15/62 , A01K2217/05 , A23K20/147 , A23K20/189 , C07K14/4723 , C07K2319/00 , C07K2319/02 , C12N15/79 , C12Q1/025
摘要： The invention relates to the use of co-expression of an antimicrobial agent and an enzyme, with a view to improving yield and/or overall production economy. Examples of antimicrobial agents are antimicrobial peptides such as lactoferricins and antimicrobial enzymes such as lysozyme and glucose oxidase, and examples of enzymes are endoglucanase, xylanase, phytase, protease, galactanase, mannanase, dextranase, alpha-galactosidase, pectate lyase, alpha-amylase and glucoamylase. A fusion product comprising the antimicrobial agent and the enzyme and a cleavable linker is novel, and can be used in animal feed and animal feed additives. The invention also describes the use of a protection domain wherein at least 50% of the amino acid residues comprised in the peptide protection domain are D (Asp) and/or E (Glu). The protection or quenching domain serves to temporarily and reversibly inactivate the antimicrobial peptide during the expression.
摘要翻译： 本发明涉及抗微生物剂和酶的共表达的用途，以提高产量和/或整体生产经济性。 抗微生物剂的实例是抗微生物肽如乳铁蛋白和抗菌酶如溶菌酶和葡萄糖氧化酶，酶的实例是内切葡聚糖酶，木聚糖酶，植酸酶，蛋白酶，半乳聚糖酶，甘露聚糖酶，葡聚糖酶，α-半乳糖苷酶，果胶酸裂合酶，α-淀粉酶和 糖化酶。 包含抗微生物剂和酶和可切割接头的融合产物是新颖的，并且可以用于动物饲料和动物饲料添加剂中。 本发明还描述了保护结构域的用途，其中肽保护结构域中包含的至少50％的氨基酸残基是D（Asp）和/或E（Glu）。 保护或猝灭结构域用于在表达过程中暂时和可逆地灭活抗微生物肽。
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公开(公告)号：EP1794295B1
公开(公告)日：2011-11-16
申请号：EP05784167.8
申请日：2005-09-21
申请人： Novozymes A/S
摘要： The present invention relates to novel JP170 like subtilases from wild-type bacteria, hybrids thereof and to methods of construction and production of these proteases. Further, the present invention relates to use of the claimed subtilases in detergents, such as a laundry or an automatic dishwashing detergent.
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公开(公告)号：EP1831396A2
公开(公告)日：2007-09-12
申请号：EP05820807.5
申请日：2005-12-20
申请人： Novozymes A/S
发明人： NIELSEN, Jesper, Duus , NIELSEN, Allan, Kent , CHRISTENSEN, Bjarke , PEDERSEN, Poul, Erik , HOEGENHAUG, Hans-Henrik, Kristensen
CPC分类号： C12N15/1086 , C12Q1/6809 , C12Q1/6897 , C12Q2565/501
摘要： The present invention relates to a method of identifying control sequences responding to an-timicrobial peptides (AMPs), to host cells expressing an AMP and comprising a control se-quence of the invention operable linked to a reporter, and to a method of screening for novel AMPs or AMP-variants having improved antimicrobial activity. Particularly the present inven-tion relates to DNA control sequences, wherein the transcriptional profiles of said control se-quences are regulated by the presence of an antimicrobial polypeptide expressed inside the host cell.
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公开(公告)号：EP1315806A1
公开(公告)日：2003-06-04
申请号：EP01960178.0
申请日：2001-07-17
申请人： Novozymes A/S
CPC分类号： C12N9/6408 , C07K14/811 , C12N15/1034 , C12Q1/025 , C12Q1/37
摘要： A method of screening a protease gene library for a gene encoding a protease of interest, the method comprising the steps of introducing a first gene of the protease gene library and a second gene encoding a protease inhibitor into a host cell; cultivating the host cell, wherein the cell expresses the first and the second genes to produce a complex of a protease and the inhibitor; dissociating the inhibitor from the complex; and selecting the protease of interest and isolating the encoding gene.
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公开(公告)号：EP3011044A1
公开(公告)日：2016-04-27
申请号：EP14733598.8
申请日：2014-06-23
申请人： Novozymes A/S
CPC分类号： C12N15/75 , C12N9/1051 , C12N9/82 , C12P21/00 , C12Y204/01 , C12Y305/01001
摘要： The present invention relates to a method of producing a natively non-secreted polypeptide without a secretion signal in a Bacillus host cell and recovering the polypeptide without performing a lysis step as well as to a Bacillus host cell cell comprising one or more exogenous or heterologous polynucleotides encoding a natively non-secreted polypeptide with no secretion signal.
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公开(公告)号：EP1315806B1
公开(公告)日：2005-12-28
申请号：EP01960178.0
申请日：2001-07-17
申请人： Novozymes A/S
CPC分类号： C12N9/6408 , C07K14/811 , C12N15/1034 , C12Q1/025 , C12Q1/37
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公开(公告)号：EP1313862A1
公开(公告)日：2003-05-28
申请号：EP01951447.0
申请日：2001-07-09
申请人： Novozymes A/S
CPC分类号： C11D3/386
摘要： The present invention provides a method for producing a protein complex comprising the steps of constructing a fusion polynucleotide sequence in frame, the sequence comprising a first gene encoding a protease, and a second gene encoding a protease inhibitor; introducing the sequence into a host cell; cultivating the host cell, wherein the cell expresses the sequence and produces a non-covalently linked complex of the protease and the inhibitor; and recovering the complex.
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9.发明授权STABLY MAINTAINED MULTIPLE COPIES OF AT LEAST TWO ORF IN THE SAME ORIENTATION 有权迈克尔·温普森WENIGSTENS ZWEIER OFFENER LESERRAHMEN STABIL ERHALTEN IN GLEICHER ORIENTIERUNG
公开(公告)号：EP1766002B1
公开(公告)日：2015-11-18
申请号：EP05750839.2
申请日：2005-06-20
申请人： Novozymes A/S
CPC分类号： C12N9/52 , C12N15/67 , C12N15/902 , C12N2800/40 , C12N2830/20
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公开(公告)号：EP1794297B1
公开(公告)日：2011-02-23
申请号：EP05784169.4
申请日：2005-09-21
申请人： Novozymes A/S
摘要： The present invention relates to novel JP170 like subtilases from wild-type bacteria, hybrids thereof and to methods of construction and production of these proteases. Further, the present invention relates to use of the claimed subtilases in detergents, such as a laundry or an automatic dishwashing detergent.
摘要翻译： 本发明涉及来自野生型细菌的新型JP170类枯草杆菌酶，其杂交体以及构建和生产这些蛋白酶的方法。 此外，本发明涉及所要求保护的枯草杆菌酶在洗涤剂如衣物或自动餐具洗涤剂中的用途。
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