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13 条记录 (耗时 0.029 秒)

    SUBTILASES AND SUBTILASE VARIANTS HAVING ALTERED IMMUNOGENICITY
    3.
    发明公开
    SUBTILASES AND SUBTILASE VARIANTS HAVING ALTERED IMMUNOGENICITY 审中-公开
    枯草杆菌和枯草杆菌蛋白酶与改进的免疫原性

    公开(公告)号：EP1520017A2

    公开(公告)日：2005-04-06

    申请号：EP03732260.9

    申请日：2003-06-25

    申请人： Novozymes A/S

    发明人： ROGGEN, Erwin, Ludo ,  NILSSON, Nina, Teeres ,  ERNST, Steffen ,  ANDERSEN, Carsten ,  BERG, Ninna, Willestofte

    IPC分类号： C12N9/54

    CPC分类号： C12N9/54 ,  C11D3/386

    摘要： The present invention relates to subtilase variants and subtilases with an altered immunogenicity, particularly subtilase variants and subtilases with a reduced allergenecity. The subtilase variant has position 57 modified in combination with a modification in at least one of the positions 170, 181 and 247. The position numbers used refer to the positions of Subtilisin Novo (BPNAE) from Bacillus amyloliquefaciens. Furthermore, the invention relates to expression of said subtilase variants and subtilases and to their use, such as in detergents and oral care products.

    GROUP 1 MITE POLYPEPTIDE VARIANTS
    4.
    发明公开
    GROUP 1 MITE POLYPEPTIDE VARIANTS 审中-公开
    变应原1-MILBENPOLYPEPTIDS

    公开(公告)号：EP1756150A2

    公开(公告)日：2007-02-28

    申请号：EP05731876.8

    申请日：2005-04-21

    申请人： Novozymes A/S

    发明人： BATORI, Vincent ,  ROGGEN, Erwin, Ludo ,  ERNST, Steffen ,  LYNGSTRAND, Stina, Thulesen

    IPC分类号： C07K14/435 ,  C12N15/12 ,  A61K39/35

    CPC分类号： C07K14/43531 ,  A61K39/00 ,  A61K2039/57

    摘要： This invention concerns variants of a group 1 mite polypeptide, wherein the mature polypeptide of the variants comprise one or more mutations in the positions or corresponding to positions consisting of P11, I14, D15, L16, M19-P24, Q28, F37, S38, T43, A46-A49, Q53-L57, V63, A66-H69, H72, D74-R77, I80, Y82, Q84, H85, S92, I113, S114, P121, V124, K126, R128-A130, A132-S136, A139, L147, A149-H152, T157, Q160, N163, H170, A171, S178, V183, D184, R189, D193, F204, A206, N207, P217, L222 of SEQ ID NO: 1 or alternatively 11, 14, 15, 16, 19-24, 28, 37, 38, 43, 46-49, 53-57, 63, 66-69, 72, 74-77, 80, 82, 84, 85, 92, 113, 114, 121, 124, 126, 128-130, 132-136, 139, 147, 149-152, 157, 160, 163, 170, 178, 183, 189, 193, 204, 206, 207, 217, 222 of the mature Der p 1 polypeptide.

    摘要翻译： 本发明涉及组1螨多肽的变体，其中变体的成熟多肽包含位置或对应于由P11，I14，D15，L16，M19-P24，Q28，F37，S38， T43，A46-A49，Q53-L57，V63，A66-H69，H72，D74-R77，I80，Y82，Q84，H85，S92，I113，S114，P121，V124，K126，R128-A130，A132-S136， SEQ ID NO：1的A139，L147，A149-H152，T157，Q160，N163，H170，A171，S178，V183，D184，R189，D193，F204，A206，N207，P217，L222或者11,14,15 ，16,19-24,28,37,38,43,46-49,53-57,63,66-69,72,74-77,80,82,84,85,92,113,114,121 ，124,126,128-130,132-136,139,147,149-152,157,160,163,170,178,183,189,193,204,206,207,217,222的成熟Der p 1多肽。

    HIGH THROUGHPUT SCREENING (HTS) ASSAYS FOR PROTEIN VARIANTS WITH REDUCED ANTIBODY BINDING CAPACITY
    7.
    发明公开
    HIGH THROUGHPUT SCREENING (HTS) ASSAYS FOR PROTEIN VARIANTS WITH REDUCED ANTIBODY BINDING CAPACITY 审中-公开
    具有高周转率（HTS）与蛋白质绑定选项ANTIKÖRPEN能力降低PROCEDURE

    公开(公告)号：EP1240513A2

    公开(公告)日：2002-09-18

    申请号：EP00981185.2

    申请日：2000-12-08

    申请人： Novozymes A/S

    发明人： ROGGEN, Erwin, Ludo ,  ERNST, Steffen ,  PEDERSEN, Henrik

    IPC分类号： G01N33/50 ,  G01N33/68 ,  C12N15/00 ,  C07K16/00

    CPC分类号： C40B30/04 ,  G01N33/6803 ,  G01N33/6842 ,  G01N33/6845

    摘要： The present invention is to provide a method to perform assays that efficiently and accurately can screen large numbers of cell populations producing variants of a molecule of interest. The present invention relates more specifically to a method for screening a library of protein variants for functional variants with reduced antibody binding capacity, comprising the steps of: (i) generating a diversified library of protein variants starting from a relevant protein backbone, (ii) transforming the library into suitable host cells, (iii) culturing host cells, (iv) sampling each cell culture, (v) analysing a sample by determining the antibody binding capacity of the variant protein, (vi) analysing a sample by determining the functionality of the variant protein.

    GROUP 1 MITE POLYPEPTIDE VARIANTS
    9.
    发明公开
    GROUP 1 MITE POLYPEPTIDE VARIANTS 审中-公开
    VARIANTE DES TYP 1 MILBEPOLYPEPTIDS

    公开(公告)号：EP1620461A2

    公开(公告)日：2006-02-01

    申请号：EP04729048.1

    申请日：2004-04-23

    申请人： Novozymes A/S

    发明人： SONI, Nanna, Kristensen ,  FRIIS, Esben, Peter ,  ROGGEN, Erwin, Ludo ,  LYNGSTRAND, Stina, Thulesen

    IPC分类号： C07K14/435 ,  C12N15/12 ,  A61K38/17 ,  A61K39/35

    CPC分类号： C07K14/43531 ,  A61K38/00 ,  A61K39/00

    摘要： This invention concerns variants of group 1 mite polypeptides, wherein the mature polypeptide of the variant comprise one or more mutations in the positions or corresponding to the positions consisting of A10, A12, E13, G29, G30, G32, A46, Y47, S54, L55, D64, A66, S67, G73, T75, I80, Q84, N86, G87, S92, Y93, Y96, A98, R99, E100, Q101, R104, R105, P106, Q109, R110, F111, G112, I113, A132, I144, K145, D146, D148, R151, I158, I159, Q160, R161, D162, N163, G164, Y165, Q166, N179, A180, G182, V183, D184, A205, I208 of SEQ ID NO: 1 or 10, 12, 13, 29, 30, 32, 46, 47, 54, 55, 64, 66, 67, 73, 75, I80, 84, 86, 87, 92, Y93, 96, 98, 99, 100, 101, 104, 105, 106, 109, 110, 111, 112, 113, 132, 144, 145, 146, 148, 151, 158, 159, 160, 161, 162, 163, 164, 165, 166, 179, 180, 182, 183, 184, 205, 208 of the mature Der p 1 polypeptide.

    摘要翻译： 本发明涉及组1螨多肽的变体，其中变体的成熟多肽在A10，A12，E13，G29，G30，G32，A46，Y47，S54， L55，D64，A66，S67，G73，T75，I80，Q84，N86，G87，S92，Y93，Y96，A98，R99，E100，Q101，R104，R105，P106，Q109，R110，F111，G112， 或SEQ ID NO：1的A132，I144，K145，D146，D148，R151，I158，I159，Q160，R161，D162，N163，G164，Y165，Q166，N179，A180，G182，V183，D184，A205， 10，12，13，29，30，32，46，47，54，55，64，66，67，73，75，I80，84，86，87，92，Y93,96,98,99,100， 101,104,105,106,109,110,111,112,113,132,144,145,146,148,151,158,159,160,161,162,163,164,165,166,179， 180,182,183,184,205,208的成熟Der p 1多肽。

    TRANSGENIC PLANTS
    10.
    发明公开
    TRANSGENIC PLANTS 审中-公开
    转基因植物

    公开(公告)号：EP1328544A1

    公开(公告)日：2003-07-23

    申请号：EP01978221.8

    申请日：2001-10-17

    申请人： Novozymes A/S

    发明人： KJAERUFF, Soren ,  ROGGEN, Erwin, Ludo

    IPC分类号： C07K14/415 ,  C12N15/82 ,  A01H5/00

    CPC分类号： C07K14/415 ,  C12N15/8257 ,  Y02A50/54

    摘要： The present invention relates to a method of producing a transgenic plant expressing a protein variant having modified immunogenicity as compared to the parent protein comprising the steps obtaining antibody binding peptide sequences in-volved in antibody binding, using the sequences to localise epitope sequences on the primaery and/or the 3-dimensional structure of parent protein, defining an epitope area includ-ing amino acids situated within 5 ANGSTROM from the epitope amino ac-ids constituting the epitope sequence, changing one or more of the amino acids defining the epitope area of the parent pro-tein by genetical engineering mutations of a DNA sequence en-coding the parent protein, introducing the mutated DNA se-quence into a suitable host, culturing said host and express-ing the protein variant, evaluating the immunogenicity of the protein variant using the parent protein as reference, intro-ducing the mutated DNA sequence into an expression construct and transforming a suitable plant cell with the construct, and regenerating the plant from the plant cell. The invention will provide less allergenic foods.

    摘要翻译： 本发明涉及产生表达蛋白质变体的转基因植物的方法，所述蛋白质变体与亲本蛋白质相比具有修饰的免疫原性，所述方法包括以下步骤：使用所述序列将表位序列定位于初始位置上的抗体结合肽序列 和/或亲本蛋白质的三维结构，定义包含位于构成表位序列的表位氨基酸5内的氨基酸的表位区域，改变定义表位区域的一个或多个氨基酸 亲本蛋白质通过编码亲本蛋白质的DNA序列的遗传工程突变，将突变的DNA序列引入合适的宿主，培养所述宿主并表达蛋白质变体，评估蛋白质变体的免疫原性 使用亲本蛋白作为参考，将突变的DNA序列引入表达构建体并转化合适的植物 细胞与构建体结合，并从植物细胞再生植物。 本发明将提供较少过敏性食物。