利索-全球专利检索

  1. Login
  2. Sign Up

Search Results

27 records (took 0.018 seconds)

    PEPTIDE ANTIBIOTIC OVER-PRODUCING STRAIN, PREPARATION METHOD THEREFOR, AND USE THEREOF
    13.
    发明公开
    PEPTIDE ANTIBIOTIC OVER-PRODUCING STRAIN, PREPARATION METHOD THEREFOR, AND USE THEREOF 有权
    应变生产肽抗生素，用于生产及其用途

    公开(公告)号：EP2653532A1

    公开(公告)日：2013-10-23

    申请号：EP11849471.5

    申请日：2011-12-15

    Applicant: Shanghai Techwell Biopharmaceutical Co., Ltd

    Inventor： CHEN, Yi ,  LIU, Shidong ,  ZHANG, Zhaoli ,  WANG, Chunxia ,  KANG, Jing ,  JI, Xiaoming

    IPC: C12N1/14 ,  C12P21/04 ,  C12R1/645

    CPC classification number: C07K7/54 ,  C07K7/56 ,  C12N1/14 ,  C12N15/01 ,  C12R1/645

    Abstract: High-yield antibiotics producing strain, preparation method and use thereof are provided in the present invention. The high-yield strain is a mutagenized strain derived from Colephoma empetri, and deposited in CGMCC with the accession number of CGMCC 4129. The preparation method comprises the following steps: (a) mixing a seed liquid of Colephoma empetri of Accession No. FERM BP-2635 with nitrosoguanidine to obtain a mixture A; (b) mixing said mixture A with a wall-breaking enzyme to obtain protoplasts; (c) regenerating said protoplasts to obtain single colonies; and (d) culturing said single colonies to obtain said mutagenized strain. The obtained strain has stable genetic and producing property, produces little impurities in fermentation, and is suitable for industrialization.

    Abstract translation: 高产抗生素生产菌株，其制备方法及其用途都在本发明中提供。 高产菌株是从诱变Colephoma empetri衍生，并存放于CGMCC与CGMCC 4129.的登录号的制备方法包括以下步骤的菌株：（a）将加入的Colephoma empetri的液体种子号 FERM BP-2635与nitrosoguanidines以获得混合物A; （b）将所述混合物A与破壁酶以获得原生质体; （C）再生所述的原生质体以获得单菌落; 和（d）培养所述单菌落以获得所述诱变的菌株。 将所得到的菌株具有稳定的遗传和生产性，在发酵产生很少杂质，并适合于工业化。

    HIGH YIELD ANTIBIOTICS PRODUCING FUNGUS STRAIN, PREPARATION METHOD AND USE THEREOF
    14.
    发明公开
    HIGH YIELD ANTIBIOTICS PRODUCING FUNGUS STRAIN, PREPARATION METHOD AND USE THEREOF 有权
    ERTRAGREICHER PILZSTAMMFÜR抗生素治疗，HERSTELLUNGSVERFAHREN UND VERWENDUNG

    公开(公告)号：EP2481792A1

    公开(公告)日：2012-08-01

    申请号：EP09849678.9

    申请日：2009-09-28

    Applicant: Shanghai Techwell Biopharmaceutical Co., Ltd

    Inventor： XU, Jing ,  CHEN, Yi ,  JI, Xiaoming ,  GAO, Xiaoliang ,  LIU, Shidong ,  ZHANG, Zhaoli

    IPC: C12N1/14 ,  C12R1/645 ,  C12P21/00 ,  C07K7/56

    CPC classification number: C12R1/645 ,  C07K7/56 ,  C12N15/01

    Abstract: High yield antibiotics producing fungus strain, preparation method and use thereof are provided. The fungus strain is a mutant derived from Glarea lozoyensis, and deposited in CGMCC with the accession number of CGMCC 2933. The preparation method concludes following steps:(a) mixing the culture media of Glarea lozoyensis strain ATCC 20957 with nitrosoguanidine, and obtaining mixture a; (b) mixing lywallzyme with the mixture a, and obtaining protoplasts; (c) regenerating the protoplasts, and obtaining single clones; and (d) culturing the single clones, then obtaining the mutant strain. This fungus strain has stable genetic and producing property, produces little impurities in fermentation, and is suitable to be used in industry.

    Abstract translation: 提供生产真菌菌株的高产抗生素，其制备方法和用途。 真菌菌株是来自山茱萸的突变体，保藏在CGMCC中，登录号为CGMCC 2933.制备方法结束如下：（a）将葛兰氏杆菌菌株ATCC 20957的培养基与亚硝基胍混合，得到混合物 ; （b）将裂解酶与混合物a混合，得到原生质体; （c）再生原生质体，获得单个克隆; 和（d）培养单个克隆，然后获得突变株。 这种真菌菌株具有稳定的遗传和生产性能，在发酵中产生的杂质很少，适用于工业。

    IMMOBILIZED CYCLOALIPHATIC PEPTIDE ACYLTRANSFERASE AND PREPARATION METHOD AND USES THEREOF
    17.
    发明公开
    IMMOBILIZED CYCLOALIPHATIC PEPTIDE ACYLTRANSFERASE AND PREPARATION METHOD AND USES THEREOF 审中-公开
    免疫组化免疫组化免疫组织化学

    公开(公告)号：EP2848685A1

    公开(公告)日：2015-03-18

    申请号：EP13788659.4

    申请日：2013-05-10

    Applicant: Shanghai Techwell Biopharmaceutical Co., Ltd

    Inventor： LIU, Shidong ,  ZHANG, Zhaoli ,  JI, Xiaoming

    IPC: C12N11/00 ,  C12N11/14 ,  C12N9/80 ,  C12P21/04

    CPC classification number: C12N11/14 ,  C07K7/56 ,  C12N9/80 ,  C12N9/86 ,  C12N11/08 ,  C12P21/02

    Abstract: Disclosed in the present invention are an immobilized cycloaliphatic peptide acyltransferase and a preparation method and use thereof. The cycloaliphatic peptide acyltransferase is immobilized on a carrier; the cycloaliphatic peptide acyltransferase is derived from natural or artificial mutants or variants thereof, or can be obtained by introducing a foreign cyclic acyltransferase gene and transforming thereafter; the material of the carrier is selected from an inorganic carrier or a polypropylene resin carrier. Also disclosed in the present invention are the preparation method for the immobilized cycloaliphatic peptide acyltransferase and uses thereof.

    Abstract translation: 本发明公开了一种固定化脂环族肽酰基转移酶及其制备方法和用途。 脂环族肽酰基转移酶固定在载体上; 脂环族肽酰基转移酶衍生自天然或人工突变体或其变体，或可以通过引入外源环酰基转移酶基因并在此后进行转化获得; 载体的材料选自无机载体或聚丙烯树脂载体。 本发明还公开了固定化脂环族肽酰基转移酶的制备方法及其用途。

Patent Agency Ranking