利索-全球专利检索

  1. Login
  2. Sign Up

Search Results

27 records (took 0.017 seconds)

    PROCESS FOR PURIFYING CYCLIC LIPOPEPTIDE COMPOUNDS OR SALTS THEREOF
    2.
    发明公开
    PROCESS FOR PURIFYING CYCLIC LIPOPEPTIDE COMPOUNDS OR SALTS THEREOF 有权
    VERFAHREN ZUR REINIGUNG VON ZYKLISCHEN LIPOPEPTIDVERBINDUNGEN ODER SALZEN DAVON

    公开(公告)号：EP2623511A1

    公开(公告)日：2013-08-07

    申请号：EP11828114.6

    申请日：2011-09-27

    Applicant: Shanghai Techwell Biopharmaceutical Co., Ltd

    Inventor： ZHANG, Zhaoli ,  LIU, Shidong ,  ZHUO, Zhonghao ,  JI, Xiaoming

    IPC: C07K1/14 ,  C07K7/56

    CPC classification number: C07K1/22 ,  C07K7/56

    Abstract: A process for purifying cyclic lipopeptide compounds or salts thereof comprising the steps of: (1) charging a crude compound of Formula I onto a macroporous adsorption resin; (2) washing the macroporous adsorption resin using water, an organic solvent or a mixed solution of an organic solvent and water as a washing liquid; and (3) eluting the compound of Formula I from the macroporous adsorption resin using water, an organic solvent or a mixed solution of an organic solvent and water as an eluent. The purification method has the advantages of using a small amount of organic solvents, using no silica gel, and causing little damage to the environment; the purity of the collected compound of formula I is also improved as compared with the methods previously disclosed.

    Abstract translation: 一种用于纯化环状脂肽化合物或其盐的方法，包括以下步骤：（1）将式I的粗制化合物装入大孔吸附树脂中; （2）使用水，有机溶剂或有机溶剂和水的混合溶液作为洗涤液洗涤大孔吸附树脂; 和（3）使用水，有机溶剂或有机溶剂和水的混合溶液作为洗脱剂，从大孔吸附树脂中洗脱式I化合物。 净化方法的优点是使用少量的有机溶剂，不使用硅胶，对环境几乎没有损害; 与以前公开的方法相比，所收集的式I化合物的纯度也得到改善。

    METHOD FOR PURIFYING CYCLIC LIPOPEPTIDE OR SALT THEREOF
    7.
    发明公开
    METHOD FOR PURIFYING CYCLIC LIPOPEPTIDE OR SALT THEREOF 有权
    VERFAHREN ZUR REINIGUNG EINES ZYKLISCHEN LIPOPEPTIDS ODER EINES SALZES DAVON

    公开(公告)号：EP2623611A1

    公开(公告)日：2013-08-07

    申请号：EP11828116.1

    申请日：2011-09-27

    Applicant: Shanghai Techwell Biopharmaceutical Co., Ltd

    Inventor： ZHANG, Zhaoli ,  LIU, Shidong ,  ZHUO, Zhonghao ,  JI, Xiaoming

    IPC: C12P21/02 ,  C07K7/06 ,  A61K38/12

    CPC classification number: C07K7/56

    Abstract: A method for purifying a cyclic lipopepteide or a salt thereof is provided. The method comprises the steps: (1) extracting a fermentation broth containing a compound of formula I or a salt thereof, to obtain an extract 1 after filtration or centrifugation; (2) diluting or concentrating the extract 1 under vacuum to decrease the content of the organic solvent, to obtain an extract 2; (3) loading the extract 2 onto a macroporous absorption resin; (4) washing the macroporpous adsorption resin with water, an organic solvent, or a mixture of water and an organic solvent as a washing solution; and (5) eluting the compound of formula I off from the macroporous adsorption resin with water, an organic solvent, or a mixture of water and an organic solvent as a washing solution as an eluant. Compared with the prior art, the purification method has the advantages that fewer organic solvent is used, no silica gel is used, the harm to the environment is less, and the purity of the collected compound of formula I is improved.

    Abstract translation: 提供了一种纯化环磷脂或其盐的方法。 该方法包括以下步骤：（1）提取含有式I化合物或其盐的发酵液，过滤或离心后得到提取物1; （2）真空稀释或浓缩提取物1以降低有机溶剂的含量，得到提取物2; （3）将提取物2装载到大孔吸附树脂上; （4）用水，有机溶剂或水和有机溶剂的混合物洗涤大孔吸附树脂作为洗涤液; 和（5）用水，有机溶剂或水和有机溶剂的混合物作为洗脱液从大孔吸附树脂中除去式I化合物作为洗脱剂。 与现有技术相比，纯化方法的优点是使用少量的有机溶剂，不使用硅胶，对环境的危害较小，提高了所收集的式I化合物的纯度。

Patent Agency Ranking