摘要:
A stable human serum based control for the assay of Total LD and CK and their isoenzymes. This control may be lyophilized and reconstituted and still provide the same enzyme activity prior to lyophilization, for seven (7) days after reconstitution, if stored in the dark at or about 2 to 8°C. No thiol compounds other than the amount normally used to purify CK isoenzymes are found in this highly stable control.
摘要:
An immunoassay for CK-MB is provided based on the sequential immunoinhibition first by an immobilized and then by a soluble antibody of either the CK-M or the CK-B sub-units in CK-MM, CK-MB and CK-BB, followed by an enzymatic determination of the unbound sub-unit in CK-MB (the unbound CK-BB or CK-MM having been previously removed).
摘要:
Zur Stabilisierung von Creatinkinase durch Disulfidmodifizierung setzt man Creatinkinase in beliebiger Reihenfolge a) mit einem molaren Überschuß an Disulfid wie z. B. Cystin, Homocystin, Cystinmethylester und/oder Cystamin und/oder Thiosulfonat wie Methanthiosulfonsäure-S-Methylester und b) mit einem molaren Überschuß an wasserlöslichem Kohlehydrat als Träger.
摘要:
Methods and materials use template-directed assembly of polypeptides and optionally additional reagents to analyze the functionality of membrane-associated proteins, such as, for example, portions of transmembrane proteins, membrane-associated proteins, and others proteins that bind to transmembrane proteins and membrane-associated proteins, and to analyze the effect of test compounds or mutations on the functionality of same. The methods and materials of the present application provide a more native-like environment for analyzing the functionality of membrane- associated proteins, and thus provide effective tools for studies involving the detection of the level of enzyme activity of such proteins in an environment that closely resembles the native environment in the cell, and for novel manufacturing processes.