MULTIPHASE NUCLEIC ACID AMPLIFICATION
    18.
    发明公开

    公开(公告)号:EP3640348A1

    公开(公告)日:2020-04-22

    申请号:EP19207485.4

    申请日:2013-08-30

    IPC分类号: C12Q1/6865

    摘要: There is disclosed a method of amplifying a target nucleic acid sequence present in a sample, the method comprising the steps of: (a) performing a first phase linear amplification reaction that amplifies the target nucleic acid sequence under conditions that do not support exponential amplification thereof; wherein the first phase linear amplification reaction generates a first amplification product; and (b) performing a second phase amplification reaction under conditions allowing exponential amplification of the first amplification product, thereby generating a second amplification product that comprises the target nucleic acid sequence or the complement thereof.