公开(公告)号：EP1454125A2

公开(公告)日：2004-09-08

申请号：EP02786771.2

申请日：2002-12-12

申请人： Artel, Inc.

发明人： CURTIS, Richard, H.

IPC分类号： G01N21/25 ,  B01L3/00 ,  G01F17/00 ,  G01F25/00

CPC分类号： G01N21/278 ,  B01L3/0241 ,  B01L3/5085 ,  B01L9/54 ,  G01F23/292 ,  G01F25/0084 ,  G01N21/253 ,  G01N21/274

摘要： A method and apparatus for measuring and calibrating the measurement of small volumes of liquids. The small volumes of liquid are typically dispensed from liquid delivery devices, the delivery device often having multiple channels to analyze many samples at once. The liquid samples are delivered to one or more cells, typically in a multi-well plate, and positioned in a spectrophotometer for determining an absorbance of a chromophore in the liquid sample. Based upon an absorbance measurement and the concentration of the chromophore, a path length of the liquid sample is determined, from which a volume of the sample may be calculated. The method and apparatus provide various means for correcting for differences in the dimensions and/or other factors causing a non-linear deviation from the Beer-Lambert law. A system or kit may be provided including sets of sample solutions of varying dilution ranges for calibrating different liquid volumes. The kit may further include software code for storing and analyzing the various sample solutions.

公开(公告)号：EP0711404B1

公开(公告)日：1998-01-14

申请号：EP94921455.5

申请日：1994-07-01

申请人： ARTEL, INC.

发明人： CURTIS, Richard, H. ,  RUNDELL, Ann E.

IPC分类号： G01F17/00 ,  G01F25/00 ,  B01L3/02

CPC分类号： G01F25/0092 ,  B01L3/021 ,  G01F17/00 ,  G01N21/314 ,  Y10T436/10 ,  Y10T436/108331

公开(公告)号：EP0711404A1

公开(公告)日：1996-05-15

申请号：EP94921455.0

申请日：1994-07-01

申请人： ARTEL, INC.

发明人： CURTIS, Richard, H. ,  RUNDELL, Ann E.

IPC分类号： B01L3 ,  G01F17 ,  G01F25 ,  G01N21

CPC分类号： G01F25/0092 ,  B01L3/021 ,  G01F17/00 ,  G01N21/314 ,  Y10T436/10 ,  Y10T436/108331

摘要： A reagent system for accurately determining the volume of a pipette using a photometer is disclosed. The reagent system for use with the photometer includes a first container holding a reference solution and at least one second container holding a sample solution. A container holding the reference solution having maximum absorbance at a first wavelength is positioned in the photometer. The absorbances of the container at the first wavelength and at a second wavelength are measured. Then the optical pathlength of the container at the second wavelength is calculated from the measured absorbances at the first and second wavelengths. An aliquot of the sample solution is introduced into the container with a pipette to be calibrated. The sample solution has maximum absorbance at the second wavelength. The sample solution is mixed with the reference solution to form a mixture. Then the absorbance of the mixture in the container is measured at the second wavelength. The volume of the aliquot is calculated from the absorbance of the mixture and the optical pathlength of the container. The volume of the aliquot represents the volume of the pipette.