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    REAGENT SYSTEM FOR CALIBRATION OF PIPETTES AND OTHER VOLUMETRIC MEASURING DEVICES
    2.
    发明公开
    REAGENT SYSTEM FOR CALIBRATION OF PIPETTES AND OTHER VOLUMETRIC MEASURING DEVICES 失效
    对于移液器的校准和其它体积测量试剂系统建议

    公开(公告)号：EP0711404A1

    公开(公告)日：1996-05-15

    申请号：EP94921455.0

    申请日：1994-07-01

    申请人： ARTEL, INC.

    发明人： CURTIS, Richard, H. ,  RUNDELL, Ann E.

    IPC分类号： B01L3 ,  G01F17 ,  G01F25 ,  G01N21

    CPC分类号： G01F25/0092 ,  B01L3/021 ,  G01F17/00 ,  G01N21/314 ,  Y10T436/10 ,  Y10T436/108331

    摘要： A reagent system for accurately determining the volume of a pipette using a photometer is disclosed. The reagent system for use with the photometer includes a first container holding a reference solution and at least one second container holding a sample solution. A container holding the reference solution having maximum absorbance at a first wavelength is positioned in the photometer. The absorbances of the container at the first wavelength and at a second wavelength are measured. Then the optical pathlength of the container at the second wavelength is calculated from the measured absorbances at the first and second wavelengths. An aliquot of the sample solution is introduced into the container with a pipette to be calibrated. The sample solution has maximum absorbance at the second wavelength. The sample solution is mixed with the reference solution to form a mixture. Then the absorbance of the mixture in the container is measured at the second wavelength. The volume of the aliquot is calculated from the absorbance of the mixture and the optical pathlength of the container. The volume of the aliquot represents the volume of the pipette.