公开(公告)号：EP2838533B1

公开(公告)日：2017-10-04

申请号：EP13779034.1

申请日：2013-04-16

申请人： Case Western Reserve University ,  Board of Regents of the University of Texas System ,  University of Kentucky

发明人： MARKOWITZ, Sanford ,  WILLSON, James, K.V. ,  POSNER, Bruce, A. ,  READY, Joseph ,  ZHANG, Yongyou ,  TAI, Hsin-Hsiung ,  MOSS, Melissa ,  ANTCZAK, Monika ,  GERSON, Stanton ,  DESAI, Amar ,  BAE, Kibeom ,  YANG, Sung Yeun

IPC分类号： A61K31/4365 ,  A61K31/4462 ,  C07D495/04 ,  A61P17/02 ,  A61P29/00

CPC分类号： C07D495/04 ,  A61K31/4365 ,  A61K31/519 ,  C07C311/21 ,  C07C2601/14 ,  C07D207/263 ,  C07D209/08 ,  C07D213/71 ,  C07D213/73 ,  C07D213/75 ,  C07D213/76 ,  C07D215/08 ,  C07D237/20 ,  C07D239/42 ,  C07D241/20 ,  C07D261/14 ,  C07D275/03 ,  C07D277/46 ,  C07D295/185 ,  C07D405/12

摘要： Compounds and methods of modulating 15-PGDH activity, modulating tissue prostaglandin levels, treating disease, diseases disorders, or conditions in which it is desired to modulate 15-PGDH activity and/or prostaglandin levels include 15-PGDH inhibitors and 15-PGDH activators described herein.

公开(公告)号：EP2417270A2

公开(公告)日：2012-02-15

申请号：EP10762298.7

申请日：2010-04-06

申请人： The Johns Hopkins University ,  Case Western Reserve University

发明人： VOGELSTEIN, Bert ,  KINZLER, Kenneth, W. ,  LI, Meng ,  DIAZ, Luis ,  PAPADOPOULOS, Nickolas ,  MARKOWITZ, Sanford

IPC分类号： C12Q1/68 ,  C12N15/11 ,  G01N33/52

CPC分类号： C12Q1/6816 ,  C12Q1/6886 ,  C12Q2600/154 ,  C12Q2565/501 ,  C12Q2527/125 ,  C12Q2523/125

摘要： Abnormal DNA methylation can be used as a biomarker in cancer patients. For such purposes, it is important to determine precisely the fraction of methylated molecules in an analyzed sample. A technology we term Methyl-BEAMing achieves this goal. Individual bisulfite-treated DNA molecules can be PCR-amplified within aqueous nanocompartments containing beads, resulting in a population of beads each containing thousands of copies of the template molecule. After hybridization with probes specific for methylated sequences, the beads can be analyzed by flow cytometry. This approach enables detection and enumeration of one methylated molecule in a population of ~5000 unmethylated molecules. Methyl-BEAMing provides digital quantification of rare methylation events and is generally applicable to the assessment of methylated genes in clinical samples.