公开(公告)号：EP0016552A1

公开(公告)日：1980-10-01

申请号：EP80300546.1

申请日：1980-02-25

申请人： Kendrew Biosystems, Inc.

发明人： Widder, Kenneth, Jon ,  Senyei, Andrew Erwin

IPC分类号： C12N1/02 ,  C12N7/02 ,  C12Q1/00

CPC分类号： C12N7/00 ,  A61K35/12 ,  C12M47/04 ,  C12N1/02 ,  C12N2710/16051 ,  G01N33/5434 ,  G01N33/544 ,  G01N2333/31 ,  G01N2333/76 ,  G01N2446/20 ,  G01N2446/30 ,  G01N2446/84 ,  Y10S436/828

摘要： Magnetically-responsive microspheres having Protein A associated with the outer surfaces thereof are reacted with antibodies selective to the cells, bacteria, or viruses to be separated from a mixed population to attach the antibodies in oriented relation with their Fab arms extending outwardly, and the microspheres are then used in a magnetic separation procedure. The preferred microspheres are prepared from a mixture of albumin, Protein A, and magnetic particles, the microspheres being prepared so that the Protein A is present in the exterior surfaces for antibody binding.

摘要翻译： Magneticaly响应微球具有与所述其外表面进行反应的抗体选择性的细胞，细菌或病毒从混合群体中分离附着在面向关系的抗体与他们的的Fab臂向外延伸，和所述微球相关的蛋白A 然后磁分离过程中使用。 首选微球体由白蛋白，蛋白A，和磁性颗粒的混合物制备，正在制备的微球所以做了蛋白A的存在对于抗体结合的外表面。