摘要:
Apparatus for analysing a liquid sample (194) comprises beam generating means (1) for generating electromagnetic radiation, detector means (8) for detecting electromagnetic radiation from the beam generating means after the radiation has interacted with the sample, and sample retaining means (10) for releasably retaining sample in the path of the beam. The sample retaining means comprises a hydrophobic surface (for example, a coating on a plate 118) on which the sample is, in use, supported. There is also disclosed a method of performing photometric or spectrophotometric analysis of a liquid sample by sandwiching samples between two opposed hydrophobic support surfaces, passing a beam of electromagnetic radiation through one of the surfaces and then through the sample, and analysing the beam after it has passed through the sample.
摘要:
An analyser comprising a source of electromagnetic radiation, a detector for said radiation and a drophead comprising a surface which is adapted to receive a drop of liquid to be tested, the drophead being positioned in use relative to the source and detector to illuminate a drop received thereon and to cause an interaction in the path of the electromagnetic radiation between the source and detector, characterised in that said surface of said drophead is dimensioned to constrain the drop to adopt a shape which is dominated more by surface tension forces than by gravitational forces.
摘要:
The invention relates to a device (1) for analysis or absorption measurement on small amounts, for example on a drop or droplet of a liquid medium (2) by means of light (3) with an upper planar location spot (4) for the application or dropping of the medium (2), a light entry (5) in the housing (6) arranged beneath said location spot or location surface (4) and a first device (7) in the light beam behind said light entry (5) for deflection of the light upwards to the location spot (4) where a detachably mounted reflector (8) is also located. The device (7) for deflecting the light beam is designed such that the direction of the optical axis of the deflected light beam is oriented upwards towards the middle (M) of the device (1) and the inclined position of the optical axis of the light beam with regard to the device mid-point (M) is arranged to be directed at the position of the reflector (8) through which the longitudinal median (M) between the light entry (5) and the light exit (1) from the device runs. The height of assembly of said device (1) relative to one in which the light is first deflected about a right angle and only then after a further direction change directed at the location spot or the sample is correspondingly lower.
摘要:
Method and apparatus of spectrophotometry or the like on extremely small samples (9) in which a drop (9) is held between two opposing surfaces (2, 7) by surface tension and one surface is controllably moved toward and away from the other. To transmit energy to and from the drop for measurement, optical fibers (6, 11) go through one or both surfaces (2, 7) and are finished flush with surfaces. One of the surfaces can be swung clear of the other for easy cleaning between tests.
摘要:
A diagnosis system, which comprises a monitor, comprising means for detecting multiple reflectance readings in an array of individual locations, means for registering an analytical cartridge in the monitor at a fixed location and orientation relative to the array, and means for determining and displaying analytical results from reflectance readings; and an analytical cartridge, comprising a liquid impervious housing, a sample application site in the housing located outside the monitor when the cartridge is registered in the monitor by the means for registering, one or more reflectance reading sites in the housing that register with one or more of the locations in the array, a capillary pathway in the housing leading from the sample application site to each of the reflectance reading sites, and a reflectance matrix located in at least one of the reflectance reading sites. In some embodiments of the invention, control features that optimize accuracy of measurement by controlling when and if sample reaches reflectance reading sites and by drawing excess sample away from undesirable locations in the cartridge are present. One control element balances the liquid-holding capacities of the application site, the sample-transporting capillary passageway that leads to the reflectance reading site, and the porous matrices from which the reflectance reading will be made, so that excess sample is excluded from entry into the cartridge while sample volumes that are below the minimum necessary for accurate operation do not reach the matrix, thereby avoiding false readings.
摘要:
Un système diagnostique, doté d'un dispositif de contrôle, comprend un dispositif permettant de détecter des lectures de réflectance multiples effectuées dans un ensemble de zones individuelles, un dispositif permettant d'introduire une cartouche d'analyse dans le dispositif de contrôle en un point et selon une orientation fixes par rapport à cet ensemble, ainsi qu'un dispositif permettant de déterminer et d'afficher des résultats d'analyse provenant des lectures de réflectance. Il comprend aussi une cartouche d'analyse munie d'un boîtier étanche aux liquides, d'un site d'application d'échantillon placé dans ce boîtier mais restant hors du dispositif de contrôle lorsque la cartouche y est insérée par le dispositif d'introduction, d'un ou plusieurs sites de lecture de réflectance placés dans le logement et entrant en contact avec une ou plusieurs zones de l'ensemble, d'un conduit capillaire placé dans le boîtier et menant du site d'application d'échantillon à chacun des sites de lecture de réflectance, ainsi que d'une matrice de réflectance placée dans l'un au moins des sites de lecture de réflectance. Dans certaines variantes de l'invention, on trouve des éléments de contrôle optimisant la précision de mesure en contrôlant le moment effectif où les échantillons parviennent aux sites de lecture de réflectance et en évacuant les échantillons surabondants hors des zones critiques de la cartouche. Un élément de contrôle équilibre les capacités en liquide du site d'application, du conduit capillaire acheminant les échantillons au site de lecture de réflectance, et des matrices poreuses sur lesquelles interviendront ces lectures de réflectance, de manière que ces échantillons surabondants ne puissent entrer dans la cartouche, alors que les volumes d'échantillons inférieurs au minimum nécessaire pour un fonctionnement précis ne peuvent parvenir à la matrice, ce qui évite ainsi toute lecture erronée.
摘要:
An image analysis system includes a video camera that collects YUV color images of a liquid sample disposed between a capital and a pedestal, the color images being collected while a light source shines light through an optical beam path between the capital and the pedestal, and a processor adapted to i) obtain from the YUV color images a grayscale component image and a light scatter component image, and ii) obtain at least one binary image of the grayscale component image and at least one binary image of the light scatter component image.
摘要:
Cell counters and methods of their use are disclosed herein. The cell counters comprise a sample mounting system that includes a base comprising a mounted lower sample surface and a cover comprising a mounted upper sample surface; a bright-field light source incorporated in the cover; an objective lens mounted below the sample mounting system; optionally, a fluorescence excitation source in optical communication with the sample mounting system; and an imaging system in optical communication with the bright-field light source and the objective lens. The mounted sample surfaces are configured for repeated use, such that disposable sample cartridges are not needed.